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Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection
Convergent evolution of phytopathogenicity is poorly described, especially among multiple strains of a single microbial species. We investigated this phenomenon with genetically diverse isolates of Fusarium oxysporum f. sp. fragariae (Fof) that cause one of two syndromes: chlorosis and wilting (the...
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley & Sons, Inc.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796522/ https://www.ncbi.nlm.nih.gov/pubmed/35706142 http://dx.doi.org/10.1111/1462-2920.16101 |
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author | Jenner, Bradley N. Henry, Peter M. |
author_facet | Jenner, Bradley N. Henry, Peter M. |
author_sort | Jenner, Bradley N. |
collection | PubMed |
description | Convergent evolution of phytopathogenicity is poorly described, especially among multiple strains of a single microbial species. We investigated this phenomenon with genetically diverse isolates of Fusarium oxysporum f. sp. fragariae (Fof) that cause one of two syndromes: chlorosis and wilting (the ‘yellows‐fragariae’ pathotype), or only wilting (the ‘wilt‐fragariae’ pathotype). We challenged strawberry (Fragaria × ananassa) plants to root infection by five fungal isolates: three yellows‐fragariae, one wilt‐fragariae and one that is not pathogenic to strawberry. All Fof isolates had chromosome‐level assemblies; three were newly generated. The two pathotypes triggered distinct host responses, especially among phytohormone‐associated genes; yellows‐fragariae isolates strongly induced jasmonic acid‐associated genes, whereas the wilt‐fragariae isolate primarily induced ethylene biosynthesis and signalling. The differentially expressed genes on fungal accessory chromosomes were almost entirely distinct between pathotypes. We identified an ~150 kbp ‘pathogenicity island’ that was horizontally transferred between wilt‐fragariae strains. This predicted pathogenicity island was enriched with differentially expressed genes whose predicted functions were related to plant infection, and only one of these genes was also upregulated in planta by yellows‐fragariae isolates. These results support the conclusion that wilt‐ and yellows‐fragariae cause physiologically distinct syndromes by the expression of discrete repertoires of genes on accessory chromosomes. |
format | Online Article Text |
id | pubmed-9796522 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley & Sons, Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97965222022-12-30 Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection Jenner, Bradley N. Henry, Peter M. Environ Microbiol Research Articles Convergent evolution of phytopathogenicity is poorly described, especially among multiple strains of a single microbial species. We investigated this phenomenon with genetically diverse isolates of Fusarium oxysporum f. sp. fragariae (Fof) that cause one of two syndromes: chlorosis and wilting (the ‘yellows‐fragariae’ pathotype), or only wilting (the ‘wilt‐fragariae’ pathotype). We challenged strawberry (Fragaria × ananassa) plants to root infection by five fungal isolates: three yellows‐fragariae, one wilt‐fragariae and one that is not pathogenic to strawberry. All Fof isolates had chromosome‐level assemblies; three were newly generated. The two pathotypes triggered distinct host responses, especially among phytohormone‐associated genes; yellows‐fragariae isolates strongly induced jasmonic acid‐associated genes, whereas the wilt‐fragariae isolate primarily induced ethylene biosynthesis and signalling. The differentially expressed genes on fungal accessory chromosomes were almost entirely distinct between pathotypes. We identified an ~150 kbp ‘pathogenicity island’ that was horizontally transferred between wilt‐fragariae strains. This predicted pathogenicity island was enriched with differentially expressed genes whose predicted functions were related to plant infection, and only one of these genes was also upregulated in planta by yellows‐fragariae isolates. These results support the conclusion that wilt‐ and yellows‐fragariae cause physiologically distinct syndromes by the expression of discrete repertoires of genes on accessory chromosomes. John Wiley & Sons, Inc. 2022-06-20 2022-10 /pmc/articles/PMC9796522/ /pubmed/35706142 http://dx.doi.org/10.1111/1462-2920.16101 Text en © 2022 The Authors. Environmental Microbiology published by Society for Applied Microbiology and John Wiley & Sons Ltd. This article has been contributed to by U.S. Government employees and their work is in the public domain in the USA. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made. |
spellingShingle | Research Articles Jenner, Bradley N. Henry, Peter M. Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
title | Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
title_full | Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
title_fullStr | Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
title_full_unstemmed | Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
title_short | Pathotypes of Fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
title_sort | pathotypes of fusarium oxysporum f. sp. fragariae express discrete repertoires of accessory genes and induce distinct host transcriptional responses during root infection |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796522/ https://www.ncbi.nlm.nih.gov/pubmed/35706142 http://dx.doi.org/10.1111/1462-2920.16101 |
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