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IL‐38 orchestrates proliferation and differentiation in human keratinocytes
Interleukin (IL)‐38 is a member of the IL‐1 cytokine family with reported anti‐inflammatory activity. The highest constitutive IL‐38 expression is detected in the skin, where it is mainly produced by differentiating keratinocytes. However, little data are available regarding its biological functions...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
John Wiley and Sons Inc.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796879/ https://www.ncbi.nlm.nih.gov/pubmed/35833307 http://dx.doi.org/10.1111/exd.14644 |
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author | Mermoud, Loïc Shutova, Maria Diaz‐Barreiro, Alejandro Talabot‐Ayer, Dominique Drukala, Justyna Wolnicki, Michal Kaya, Gürkan Boehncke, Wolf‐Henning Palmer, Gaby Borowczyk, Julia |
author_facet | Mermoud, Loïc Shutova, Maria Diaz‐Barreiro, Alejandro Talabot‐Ayer, Dominique Drukala, Justyna Wolnicki, Michal Kaya, Gürkan Boehncke, Wolf‐Henning Palmer, Gaby Borowczyk, Julia |
author_sort | Mermoud, Loïc |
collection | PubMed |
description | Interleukin (IL)‐38 is a member of the IL‐1 cytokine family with reported anti‐inflammatory activity. The highest constitutive IL‐38 expression is detected in the skin, where it is mainly produced by differentiating keratinocytes. However, little data are available regarding its biological functions. In this study, we investigated the role of IL‐38 in skin physiology. We demonstrate here that dermal fibroblasts and epithelial cells of skin appendages, such as eccrine sweat glands and sebaceous glands, also express IL‐38. Next, using two‐ and three‐dimensional cell cultures, we show that endogenous expression of IL‐38 correlates with keratinocyte differentiation and its ectopic overexpression inhibits keratinocyte proliferation and enhances differentiation. Accordingly, immunohistochemical analysis revealed downregulation of IL‐38 in skin pathologies characterized by keratinocyte hyperproliferation, such as psoriasis and basal or squamous cell carcinoma. Finally, intracellular IL‐38 can shuttle between the nucleus and the cytoplasm and its overexpression modulates the activity of the transcription regulators YAP and ID1. Our results indicate that IL‐38 can act independently from immune system activation and suggest that it may affect the epidermis directly by decreasing proliferation and promoting differentiation of keratinocytes. These data suggest an important role of keratinocyte‐derived IL‐38 in skin homeostasis and pathologies characterized by epidermal alterations. |
format | Online Article Text |
id | pubmed-9796879 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | John Wiley and Sons Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97968792023-01-04 IL‐38 orchestrates proliferation and differentiation in human keratinocytes Mermoud, Loïc Shutova, Maria Diaz‐Barreiro, Alejandro Talabot‐Ayer, Dominique Drukala, Justyna Wolnicki, Michal Kaya, Gürkan Boehncke, Wolf‐Henning Palmer, Gaby Borowczyk, Julia Exp Dermatol Research Articles Interleukin (IL)‐38 is a member of the IL‐1 cytokine family with reported anti‐inflammatory activity. The highest constitutive IL‐38 expression is detected in the skin, where it is mainly produced by differentiating keratinocytes. However, little data are available regarding its biological functions. In this study, we investigated the role of IL‐38 in skin physiology. We demonstrate here that dermal fibroblasts and epithelial cells of skin appendages, such as eccrine sweat glands and sebaceous glands, also express IL‐38. Next, using two‐ and three‐dimensional cell cultures, we show that endogenous expression of IL‐38 correlates with keratinocyte differentiation and its ectopic overexpression inhibits keratinocyte proliferation and enhances differentiation. Accordingly, immunohistochemical analysis revealed downregulation of IL‐38 in skin pathologies characterized by keratinocyte hyperproliferation, such as psoriasis and basal or squamous cell carcinoma. Finally, intracellular IL‐38 can shuttle between the nucleus and the cytoplasm and its overexpression modulates the activity of the transcription regulators YAP and ID1. Our results indicate that IL‐38 can act independently from immune system activation and suggest that it may affect the epidermis directly by decreasing proliferation and promoting differentiation of keratinocytes. These data suggest an important role of keratinocyte‐derived IL‐38 in skin homeostasis and pathologies characterized by epidermal alterations. John Wiley and Sons Inc. 2022-07-25 2022-11 /pmc/articles/PMC9796879/ /pubmed/35833307 http://dx.doi.org/10.1111/exd.14644 Text en © 2022 The Authors. Experimental Dermatology published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by-nc/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc/4.0/ (https://creativecommons.org/licenses/by-nc/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited and is not used for commercial purposes. |
spellingShingle | Research Articles Mermoud, Loïc Shutova, Maria Diaz‐Barreiro, Alejandro Talabot‐Ayer, Dominique Drukala, Justyna Wolnicki, Michal Kaya, Gürkan Boehncke, Wolf‐Henning Palmer, Gaby Borowczyk, Julia IL‐38 orchestrates proliferation and differentiation in human keratinocytes |
title |
IL‐38 orchestrates proliferation and differentiation in human keratinocytes |
title_full |
IL‐38 orchestrates proliferation and differentiation in human keratinocytes |
title_fullStr |
IL‐38 orchestrates proliferation and differentiation in human keratinocytes |
title_full_unstemmed |
IL‐38 orchestrates proliferation and differentiation in human keratinocytes |
title_short |
IL‐38 orchestrates proliferation and differentiation in human keratinocytes |
title_sort | il‐38 orchestrates proliferation and differentiation in human keratinocytes |
topic | Research Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9796879/ https://www.ncbi.nlm.nih.gov/pubmed/35833307 http://dx.doi.org/10.1111/exd.14644 |
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