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Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit

Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we...

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Autores principales: Tane, Shoji, Shintomi, Keishi, Kinoshita, Kazuhisa, Tsubota, Yuko, Yoshida, Makoto M, Nishiyama, Tomoko, Hirano, Tatsuya
Formato: Online Artículo Texto
Lenguaje:English
Publicado: eLife Sciences Publications, Ltd 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797191/
https://www.ncbi.nlm.nih.gov/pubmed/36511239
http://dx.doi.org/10.7554/eLife.84694
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author Tane, Shoji
Shintomi, Keishi
Kinoshita, Kazuhisa
Tsubota, Yuko
Yoshida, Makoto M
Nishiyama, Tomoko
Hirano, Tatsuya
author_facet Tane, Shoji
Shintomi, Keishi
Kinoshita, Kazuhisa
Tsubota, Yuko
Yoshida, Makoto M
Nishiyama, Tomoko
Hirano, Tatsuya
author_sort Tane, Shoji
collection PubMed
description Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we set up a panel of in vitro assays to demonstrate that cell cycle-specific loading of condensin I is regulated by the N-terminal tail (N-tail) of its kleisin subunit CAP-H. Deletion of the N-tail accelerates condensin I loading and chromosome assembly in Xenopus egg mitotic extracts. Phosphorylation-deficient and phosphorylation-mimetic mutations in the CAP-H N-tail decelerate and accelerate condensin I loading, respectively. Remarkably, deletion of the N-tail enables condensin I to assemble mitotic chromosome-like structures even in interphase extracts. Together with other extract-free functional assays in vitro, our results uncover one of the multilayered mechanisms that ensure cell cycle-specific loading of condensin I onto chromosomes.
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spelling pubmed-97971912022-12-29 Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit Tane, Shoji Shintomi, Keishi Kinoshita, Kazuhisa Tsubota, Yuko Yoshida, Makoto M Nishiyama, Tomoko Hirano, Tatsuya eLife Chromosomes and Gene Expression Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we set up a panel of in vitro assays to demonstrate that cell cycle-specific loading of condensin I is regulated by the N-terminal tail (N-tail) of its kleisin subunit CAP-H. Deletion of the N-tail accelerates condensin I loading and chromosome assembly in Xenopus egg mitotic extracts. Phosphorylation-deficient and phosphorylation-mimetic mutations in the CAP-H N-tail decelerate and accelerate condensin I loading, respectively. Remarkably, deletion of the N-tail enables condensin I to assemble mitotic chromosome-like structures even in interphase extracts. Together with other extract-free functional assays in vitro, our results uncover one of the multilayered mechanisms that ensure cell cycle-specific loading of condensin I onto chromosomes. eLife Sciences Publications, Ltd 2022-12-13 /pmc/articles/PMC9797191/ /pubmed/36511239 http://dx.doi.org/10.7554/eLife.84694 Text en © 2022, Tane et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited.
spellingShingle Chromosomes and Gene Expression
Tane, Shoji
Shintomi, Keishi
Kinoshita, Kazuhisa
Tsubota, Yuko
Yoshida, Makoto M
Nishiyama, Tomoko
Hirano, Tatsuya
Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
title Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
title_full Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
title_fullStr Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
title_full_unstemmed Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
title_short Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
title_sort cell cycle-specific loading of condensin i is regulated by the n-terminal tail of its kleisin subunit
topic Chromosomes and Gene Expression
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797191/
https://www.ncbi.nlm.nih.gov/pubmed/36511239
http://dx.doi.org/10.7554/eLife.84694
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