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Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit
Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
eLife Sciences Publications, Ltd
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797191/ https://www.ncbi.nlm.nih.gov/pubmed/36511239 http://dx.doi.org/10.7554/eLife.84694 |
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author | Tane, Shoji Shintomi, Keishi Kinoshita, Kazuhisa Tsubota, Yuko Yoshida, Makoto M Nishiyama, Tomoko Hirano, Tatsuya |
author_facet | Tane, Shoji Shintomi, Keishi Kinoshita, Kazuhisa Tsubota, Yuko Yoshida, Makoto M Nishiyama, Tomoko Hirano, Tatsuya |
author_sort | Tane, Shoji |
collection | PubMed |
description | Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we set up a panel of in vitro assays to demonstrate that cell cycle-specific loading of condensin I is regulated by the N-terminal tail (N-tail) of its kleisin subunit CAP-H. Deletion of the N-tail accelerates condensin I loading and chromosome assembly in Xenopus egg mitotic extracts. Phosphorylation-deficient and phosphorylation-mimetic mutations in the CAP-H N-tail decelerate and accelerate condensin I loading, respectively. Remarkably, deletion of the N-tail enables condensin I to assemble mitotic chromosome-like structures even in interphase extracts. Together with other extract-free functional assays in vitro, our results uncover one of the multilayered mechanisms that ensure cell cycle-specific loading of condensin I onto chromosomes. |
format | Online Article Text |
id | pubmed-9797191 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | eLife Sciences Publications, Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-97971912022-12-29 Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit Tane, Shoji Shintomi, Keishi Kinoshita, Kazuhisa Tsubota, Yuko Yoshida, Makoto M Nishiyama, Tomoko Hirano, Tatsuya eLife Chromosomes and Gene Expression Condensin I is a pentameric protein complex that plays an essential role in mitotic chromosome assembly in eukaryotic cells. Although it has been shown that condensin I loading is mitosis specific, it remains poorly understood how the robust cell cycle regulation of condensin I is achieved. Here, we set up a panel of in vitro assays to demonstrate that cell cycle-specific loading of condensin I is regulated by the N-terminal tail (N-tail) of its kleisin subunit CAP-H. Deletion of the N-tail accelerates condensin I loading and chromosome assembly in Xenopus egg mitotic extracts. Phosphorylation-deficient and phosphorylation-mimetic mutations in the CAP-H N-tail decelerate and accelerate condensin I loading, respectively. Remarkably, deletion of the N-tail enables condensin I to assemble mitotic chromosome-like structures even in interphase extracts. Together with other extract-free functional assays in vitro, our results uncover one of the multilayered mechanisms that ensure cell cycle-specific loading of condensin I onto chromosomes. eLife Sciences Publications, Ltd 2022-12-13 /pmc/articles/PMC9797191/ /pubmed/36511239 http://dx.doi.org/10.7554/eLife.84694 Text en © 2022, Tane et al https://creativecommons.org/licenses/by/4.0/This article is distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use and redistribution provided that the original author and source are credited. |
spellingShingle | Chromosomes and Gene Expression Tane, Shoji Shintomi, Keishi Kinoshita, Kazuhisa Tsubota, Yuko Yoshida, Makoto M Nishiyama, Tomoko Hirano, Tatsuya Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit |
title | Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit |
title_full | Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit |
title_fullStr | Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit |
title_full_unstemmed | Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit |
title_short | Cell cycle-specific loading of condensin I is regulated by the N-terminal tail of its kleisin subunit |
title_sort | cell cycle-specific loading of condensin i is regulated by the n-terminal tail of its kleisin subunit |
topic | Chromosomes and Gene Expression |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797191/ https://www.ncbi.nlm.nih.gov/pubmed/36511239 http://dx.doi.org/10.7554/eLife.84694 |
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