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Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole
INTRODUCTION: Chlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the mole...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797500/ https://www.ncbi.nlm.nih.gov/pubmed/36590199 http://dx.doi.org/10.3389/fnut.2022.1091477 |
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author | Chen, Ge Wang, Ge Xu, Weidong Xiao, Ying Peng, Ye |
author_facet | Chen, Ge Wang, Ge Xu, Weidong Xiao, Ying Peng, Ye |
author_sort | Chen, Ge |
collection | PubMed |
description | INTRODUCTION: Chlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the molecular mechanisms of chlorantraniliprole-induced fat accumulation in 3T3-L1 adipocytes. METHODS: We measured the triglyceride content in chlorantraniliprole-treated 3T3-L1 adipocytes, and collected cell samples treated with chlorantraniliprole for 24 h and without any treatment for RNA sequencing. RESULTS: Compared with the control group, the content of triglyceride in the treatment group of chlorantraniliprole was significantly increased. The results of RNA sequencing (RNA-seq) showed that 284 differentially expressed genes (DEGs) were identified after treatment with chlorantraniliprole, involving 39 functional groups of gene ontology (GO) and 213 KEGG pathways. Moreover, these DEGs were significantly enriched in several key genes that regulate adipocyte differentiation and lipogenesis including Igf1, Rarres2, Nr1h3, and Psmb8. DISCUSSION: In general, these results suggest that chlorantraniliprole-induced lipogenesis is attributed to a whole-gene transcriptome response, which promotes further understanding of the potential mechanism of chlorantraniliprole-induced adipogenesis. |
format | Online Article Text |
id | pubmed-9797500 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97975002022-12-30 Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole Chen, Ge Wang, Ge Xu, Weidong Xiao, Ying Peng, Ye Front Nutr Nutrition INTRODUCTION: Chlorantraniliprole is a diamide insecticide widely used in agriculture. Chlorantraniliprole has been previously found to increase the accumulation of triglycerides (fats) in adipocytes, however, the underlying molecular mechanism is unknown. The present study aimed to explore the molecular mechanisms of chlorantraniliprole-induced fat accumulation in 3T3-L1 adipocytes. METHODS: We measured the triglyceride content in chlorantraniliprole-treated 3T3-L1 adipocytes, and collected cell samples treated with chlorantraniliprole for 24 h and without any treatment for RNA sequencing. RESULTS: Compared with the control group, the content of triglyceride in the treatment group of chlorantraniliprole was significantly increased. The results of RNA sequencing (RNA-seq) showed that 284 differentially expressed genes (DEGs) were identified after treatment with chlorantraniliprole, involving 39 functional groups of gene ontology (GO) and 213 KEGG pathways. Moreover, these DEGs were significantly enriched in several key genes that regulate adipocyte differentiation and lipogenesis including Igf1, Rarres2, Nr1h3, and Psmb8. DISCUSSION: In general, these results suggest that chlorantraniliprole-induced lipogenesis is attributed to a whole-gene transcriptome response, which promotes further understanding of the potential mechanism of chlorantraniliprole-induced adipogenesis. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797500/ /pubmed/36590199 http://dx.doi.org/10.3389/fnut.2022.1091477 Text en Copyright © 2022 Chen, Wang, Xu, Xiao and Peng. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Nutrition Chen, Ge Wang, Ge Xu, Weidong Xiao, Ying Peng, Ye Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
title | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
title_full | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
title_fullStr | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
title_full_unstemmed | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
title_short | Transcriptome analysis of fat accumulation in 3T3-L1 adipocytes induced by chlorantraniliprole |
title_sort | transcriptome analysis of fat accumulation in 3t3-l1 adipocytes induced by chlorantraniliprole |
topic | Nutrition |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797500/ https://www.ncbi.nlm.nih.gov/pubmed/36590199 http://dx.doi.org/10.3389/fnut.2022.1091477 |
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