Cargando…
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
The hard-end is a disorder of pear fruit, however, the mechanisms underlying its development remain unknown. In this study, we found that the hard-end fruit contained a higher transcript abundance level of ethylene-response factor 1b-like (PpERF1b-like) and released more ethylene compared to normal...
Autores principales: | , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797680/ https://www.ncbi.nlm.nih.gov/pubmed/36589086 http://dx.doi.org/10.3389/fpls.2022.1087388 |
_version_ | 1784860732202942464 |
---|---|
author | Jin, Xiaoshan Cheng, Chenxia Qi, Qi Zhou, Suping Wang, Caihong Zhang, Yong Sun, Chao Wang, Yuling Dang, Ruihong Yang, Shaolan |
author_facet | Jin, Xiaoshan Cheng, Chenxia Qi, Qi Zhou, Suping Wang, Caihong Zhang, Yong Sun, Chao Wang, Yuling Dang, Ruihong Yang, Shaolan |
author_sort | Jin, Xiaoshan |
collection | PubMed |
description | The hard-end is a disorder of pear fruit, however, the mechanisms underlying its development remain unknown. In this study, we found that the hard-end fruit contained a higher transcript abundance level of ethylene-response factor 1b-like (PpERF1b-like) and released more ethylene compared to normal pear. In the ethephon treated normal fruit, flesh tissues accumulated more lignin together with elevated expression of PpERF1b-like. Overexpressing PpERF1b-like transiently in fruit and stably in callus increased lignin accumulation and the expression of lignin biosynthesis genes; the opposite results were observed in fruit showing repressed expression of PpERF1b-like. These results confirmed the role of PpERF1b-like in promoting hard-end formation through promoting lignin synthesis. This study provided valuable information for further clarifying the regulation of hard-end formation in pear. |
format | Online Article Text |
id | pubmed-9797680 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97976802022-12-30 PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit Jin, Xiaoshan Cheng, Chenxia Qi, Qi Zhou, Suping Wang, Caihong Zhang, Yong Sun, Chao Wang, Yuling Dang, Ruihong Yang, Shaolan Front Plant Sci Plant Science The hard-end is a disorder of pear fruit, however, the mechanisms underlying its development remain unknown. In this study, we found that the hard-end fruit contained a higher transcript abundance level of ethylene-response factor 1b-like (PpERF1b-like) and released more ethylene compared to normal pear. In the ethephon treated normal fruit, flesh tissues accumulated more lignin together with elevated expression of PpERF1b-like. Overexpressing PpERF1b-like transiently in fruit and stably in callus increased lignin accumulation and the expression of lignin biosynthesis genes; the opposite results were observed in fruit showing repressed expression of PpERF1b-like. These results confirmed the role of PpERF1b-like in promoting hard-end formation through promoting lignin synthesis. This study provided valuable information for further clarifying the regulation of hard-end formation in pear. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797680/ /pubmed/36589086 http://dx.doi.org/10.3389/fpls.2022.1087388 Text en Copyright © 2022 Jin, Cheng, Qi, Zhou, Wang, Zhang, Sun, Wang, Dang and Yang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Jin, Xiaoshan Cheng, Chenxia Qi, Qi Zhou, Suping Wang, Caihong Zhang, Yong Sun, Chao Wang, Yuling Dang, Ruihong Yang, Shaolan PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit |
title |
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit |
title_full |
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit |
title_fullStr |
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit |
title_full_unstemmed |
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit |
title_short |
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit |
title_sort | pperf1b-like enhances lignin synthesis in pear (pyrus pyrifolia) ‘hard-end’ fruit |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797680/ https://www.ncbi.nlm.nih.gov/pubmed/36589086 http://dx.doi.org/10.3389/fpls.2022.1087388 |
work_keys_str_mv | AT jinxiaoshan pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT chengchenxia pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT qiqi pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT zhousuping pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT wangcaihong pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT zhangyong pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT sunchao pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT wangyuling pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT dangruihong pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit AT yangshaolan pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit |