Cargando…

PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit

The hard-end is a disorder of pear fruit, however, the mechanisms underlying its development remain unknown. In this study, we found that the hard-end fruit contained a higher transcript abundance level of ethylene-response factor 1b-like (PpERF1b-like) and released more ethylene compared to normal...

Descripción completa

Detalles Bibliográficos
Autores principales: Jin, Xiaoshan, Cheng, Chenxia, Qi, Qi, Zhou, Suping, Wang, Caihong, Zhang, Yong, Sun, Chao, Wang, Yuling, Dang, Ruihong, Yang, Shaolan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797680/
https://www.ncbi.nlm.nih.gov/pubmed/36589086
http://dx.doi.org/10.3389/fpls.2022.1087388
_version_ 1784860732202942464
author Jin, Xiaoshan
Cheng, Chenxia
Qi, Qi
Zhou, Suping
Wang, Caihong
Zhang, Yong
Sun, Chao
Wang, Yuling
Dang, Ruihong
Yang, Shaolan
author_facet Jin, Xiaoshan
Cheng, Chenxia
Qi, Qi
Zhou, Suping
Wang, Caihong
Zhang, Yong
Sun, Chao
Wang, Yuling
Dang, Ruihong
Yang, Shaolan
author_sort Jin, Xiaoshan
collection PubMed
description The hard-end is a disorder of pear fruit, however, the mechanisms underlying its development remain unknown. In this study, we found that the hard-end fruit contained a higher transcript abundance level of ethylene-response factor 1b-like (PpERF1b-like) and released more ethylene compared to normal pear. In the ethephon treated normal fruit, flesh tissues accumulated more lignin together with elevated expression of PpERF1b-like. Overexpressing PpERF1b-like transiently in fruit and stably in callus increased lignin accumulation and the expression of lignin biosynthesis genes; the opposite results were observed in fruit showing repressed expression of PpERF1b-like. These results confirmed the role of PpERF1b-like in promoting hard-end formation through promoting lignin synthesis. This study provided valuable information for further clarifying the regulation of hard-end formation in pear.
format Online
Article
Text
id pubmed-9797680
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97976802022-12-30 PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit Jin, Xiaoshan Cheng, Chenxia Qi, Qi Zhou, Suping Wang, Caihong Zhang, Yong Sun, Chao Wang, Yuling Dang, Ruihong Yang, Shaolan Front Plant Sci Plant Science The hard-end is a disorder of pear fruit, however, the mechanisms underlying its development remain unknown. In this study, we found that the hard-end fruit contained a higher transcript abundance level of ethylene-response factor 1b-like (PpERF1b-like) and released more ethylene compared to normal pear. In the ethephon treated normal fruit, flesh tissues accumulated more lignin together with elevated expression of PpERF1b-like. Overexpressing PpERF1b-like transiently in fruit and stably in callus increased lignin accumulation and the expression of lignin biosynthesis genes; the opposite results were observed in fruit showing repressed expression of PpERF1b-like. These results confirmed the role of PpERF1b-like in promoting hard-end formation through promoting lignin synthesis. This study provided valuable information for further clarifying the regulation of hard-end formation in pear. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797680/ /pubmed/36589086 http://dx.doi.org/10.3389/fpls.2022.1087388 Text en Copyright © 2022 Jin, Cheng, Qi, Zhou, Wang, Zhang, Sun, Wang, Dang and Yang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Jin, Xiaoshan
Cheng, Chenxia
Qi, Qi
Zhou, Suping
Wang, Caihong
Zhang, Yong
Sun, Chao
Wang, Yuling
Dang, Ruihong
Yang, Shaolan
PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
title PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
title_full PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
title_fullStr PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
title_full_unstemmed PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
title_short PpERF1b-like enhances lignin synthesis in pear (Pyrus pyrifolia) ‘hard-end’ fruit
title_sort pperf1b-like enhances lignin synthesis in pear (pyrus pyrifolia) ‘hard-end’ fruit
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797680/
https://www.ncbi.nlm.nih.gov/pubmed/36589086
http://dx.doi.org/10.3389/fpls.2022.1087388
work_keys_str_mv AT jinxiaoshan pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT chengchenxia pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT qiqi pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT zhousuping pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT wangcaihong pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT zhangyong pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT sunchao pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT wangyuling pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT dangruihong pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit
AT yangshaolan pperf1blikeenhancesligninsynthesisinpearpyruspyrifoliahardendfruit