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Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury
INTRODUCTION: Spinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797719/ https://www.ncbi.nlm.nih.gov/pubmed/36590290 http://dx.doi.org/10.3389/fnins.2022.1065897 |
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author | Qin, Chuan Liu, Yi Xu, Pei-Pei Zhang, Xin Talifu, Zuliyaer Liu, Jia-Yi Jing, Ying-Li Bai, Fan Zhao, Li-Xi Yu, Yan Gao, Feng Li, Jian-Jun |
author_facet | Qin, Chuan Liu, Yi Xu, Pei-Pei Zhang, Xin Talifu, Zuliyaer Liu, Jia-Yi Jing, Ying-Li Bai, Fan Zhao, Li-Xi Yu, Yan Gao, Feng Li, Jian-Jun |
author_sort | Qin, Chuan |
collection | PubMed |
description | INTRODUCTION: Spinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat model after traumatic SCI. METHODS: In this study, we first selected and overexpressed target circRNA in rats. We then explored its functional roles using various functional assays in a rat model after SCI. RESULTS: We found that rno-circRNA-013017—the selected target circRNA—reduced neuron apoptosis, preserved the survival and activity of motor neurons, and regulated apoptosis-related proteins at 3 days post-SCI using western blot, immunofluorescence and polymerase chain reaction. Additionally, we found that rno-circRNA-013017 inhibited descending axonal degeneration and preserved motor neurons and descending axons at 6 weeks post-SCI using immunofluorescence, biotin dextran amine diffusion tensor imaging. Finally, the overexpression of rno-circRNA-013017 promoted the locomotor function of rats after SCI using open-field test and gait analysis. CONCLUSION: Focusing on the functions of rno-circRNA-013017, this study provides new options for future studies exploring therapeutic targets and molecular mechanisms for SCI. |
format | Online Article Text |
id | pubmed-9797719 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97977192022-12-30 Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury Qin, Chuan Liu, Yi Xu, Pei-Pei Zhang, Xin Talifu, Zuliyaer Liu, Jia-Yi Jing, Ying-Li Bai, Fan Zhao, Li-Xi Yu, Yan Gao, Feng Li, Jian-Jun Front Neurosci Neuroscience INTRODUCTION: Spinal cord injury (SCI) often causes continuous neurological damage to clinical patients. Circular RNAs (circRNAs) are related to a lot of diseases, including SCI. We previously found five candidate circRNAs which were likely to regulate the secondary pathophysiological changes in rat model after traumatic SCI. METHODS: In this study, we first selected and overexpressed target circRNA in rats. We then explored its functional roles using various functional assays in a rat model after SCI. RESULTS: We found that rno-circRNA-013017—the selected target circRNA—reduced neuron apoptosis, preserved the survival and activity of motor neurons, and regulated apoptosis-related proteins at 3 days post-SCI using western blot, immunofluorescence and polymerase chain reaction. Additionally, we found that rno-circRNA-013017 inhibited descending axonal degeneration and preserved motor neurons and descending axons at 6 weeks post-SCI using immunofluorescence, biotin dextran amine diffusion tensor imaging. Finally, the overexpression of rno-circRNA-013017 promoted the locomotor function of rats after SCI using open-field test and gait analysis. CONCLUSION: Focusing on the functions of rno-circRNA-013017, this study provides new options for future studies exploring therapeutic targets and molecular mechanisms for SCI. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797719/ /pubmed/36590290 http://dx.doi.org/10.3389/fnins.2022.1065897 Text en Copyright © 2022 Qin, Liu, Xu, Zhang, Talifu, Liu, Jing, Bai, Zhao, Yu, Gao and Li. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Qin, Chuan Liu, Yi Xu, Pei-Pei Zhang, Xin Talifu, Zuliyaer Liu, Jia-Yi Jing, Ying-Li Bai, Fan Zhao, Li-Xi Yu, Yan Gao, Feng Li, Jian-Jun Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
title | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
title_full | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
title_fullStr | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
title_full_unstemmed | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
title_short | Inhibition by rno-circRNA-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
title_sort | inhibition by rno-circrna-013017 of the apoptosis of motor neurons in anterior horn and descending axonal degeneration in rats after traumatic spinal cord injury |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797719/ https://www.ncbi.nlm.nih.gov/pubmed/36590290 http://dx.doi.org/10.3389/fnins.2022.1065897 |
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