Cargando…

Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract

Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestr...

Descripción completa

Detalles Bibliográficos
Autores principales: Hrabar, Jerko, Petrić, Mirela, Cavallero, Serena, Salvemini, Marco, D’Amelio, Stefano, Mladineo, Ivona
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797851/
https://www.ncbi.nlm.nih.gov/pubmed/36590595
http://dx.doi.org/10.3389/fcimb.2022.1042679
_version_ 1784860774079922176
author Hrabar, Jerko
Petrić, Mirela
Cavallero, Serena
Salvemini, Marco
D’Amelio, Stefano
Mladineo, Ivona
author_facet Hrabar, Jerko
Petrić, Mirela
Cavallero, Serena
Salvemini, Marco
D’Amelio, Stefano
Mladineo, Ivona
author_sort Hrabar, Jerko
collection PubMed
description Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestrial host, we stimulated peripheral blood leukocytes (PBLs) of fish (paratenic host) and rat (accidental, human-model host) with A. pegreffii crude extract and analysed PBL transcriptomes 1 and 12 h post-stimulation. Fish and rat PBLs differentially expressed 712 and 493 transcripts, respectively, between 1 and 12 h post-stimulation (false discovery rate, FDR <0.001, logFC >2). While there was a difference in the highest upregulated transcripts between two time-points, the same Gene Ontologies, biological processes (intracellular signal transduction, DNA-dependent transcription, and DNA-regulated regulation of transcription), and molecular functions (ATP and metal ion binding) were enriched in the two hosts, showing an incrementing dynamic between 1 and 12 h. This suggests that the two distinct hosts employ qualitatively different transcript cascades only to achieve the same effect, at least during an early innate immunity response. Activation of later immunity elements and/or a combination of other host’s intrinsic conditions may contribute to the death of L3 in the terrestrial host.
format Online
Article
Text
id pubmed-9797851
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-97978512022-12-30 Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract Hrabar, Jerko Petrić, Mirela Cavallero, Serena Salvemini, Marco D’Amelio, Stefano Mladineo, Ivona Front Cell Infect Microbiol Cellular and Infection Microbiology Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestrial host, we stimulated peripheral blood leukocytes (PBLs) of fish (paratenic host) and rat (accidental, human-model host) with A. pegreffii crude extract and analysed PBL transcriptomes 1 and 12 h post-stimulation. Fish and rat PBLs differentially expressed 712 and 493 transcripts, respectively, between 1 and 12 h post-stimulation (false discovery rate, FDR <0.001, logFC >2). While there was a difference in the highest upregulated transcripts between two time-points, the same Gene Ontologies, biological processes (intracellular signal transduction, DNA-dependent transcription, and DNA-regulated regulation of transcription), and molecular functions (ATP and metal ion binding) were enriched in the two hosts, showing an incrementing dynamic between 1 and 12 h. This suggests that the two distinct hosts employ qualitatively different transcript cascades only to achieve the same effect, at least during an early innate immunity response. Activation of later immunity elements and/or a combination of other host’s intrinsic conditions may contribute to the death of L3 in the terrestrial host. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797851/ /pubmed/36590595 http://dx.doi.org/10.3389/fcimb.2022.1042679 Text en Copyright © 2022 Hrabar, Petrić, Cavallero, Salvemini, D’Amelio and Mladineo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Hrabar, Jerko
Petrić, Mirela
Cavallero, Serena
Salvemini, Marco
D’Amelio, Stefano
Mladineo, Ivona
Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
title Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
title_full Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
title_fullStr Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
title_full_unstemmed Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
title_short Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
title_sort rat and fish peripheral blood leukocytes respond distinctively to anisakis pegreffii (nematoda, anisakidae) crude extract
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797851/
https://www.ncbi.nlm.nih.gov/pubmed/36590595
http://dx.doi.org/10.3389/fcimb.2022.1042679
work_keys_str_mv AT hrabarjerko ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract
AT petricmirela ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract
AT cavalleroserena ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract
AT salveminimarco ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract
AT dameliostefano ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract
AT mladineoivona ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract