Cargando…
Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract
Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestr...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797851/ https://www.ncbi.nlm.nih.gov/pubmed/36590595 http://dx.doi.org/10.3389/fcimb.2022.1042679 |
_version_ | 1784860774079922176 |
---|---|
author | Hrabar, Jerko Petrić, Mirela Cavallero, Serena Salvemini, Marco D’Amelio, Stefano Mladineo, Ivona |
author_facet | Hrabar, Jerko Petrić, Mirela Cavallero, Serena Salvemini, Marco D’Amelio, Stefano Mladineo, Ivona |
author_sort | Hrabar, Jerko |
collection | PubMed |
description | Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestrial host, we stimulated peripheral blood leukocytes (PBLs) of fish (paratenic host) and rat (accidental, human-model host) with A. pegreffii crude extract and analysed PBL transcriptomes 1 and 12 h post-stimulation. Fish and rat PBLs differentially expressed 712 and 493 transcripts, respectively, between 1 and 12 h post-stimulation (false discovery rate, FDR <0.001, logFC >2). While there was a difference in the highest upregulated transcripts between two time-points, the same Gene Ontologies, biological processes (intracellular signal transduction, DNA-dependent transcription, and DNA-regulated regulation of transcription), and molecular functions (ATP and metal ion binding) were enriched in the two hosts, showing an incrementing dynamic between 1 and 12 h. This suggests that the two distinct hosts employ qualitatively different transcript cascades only to achieve the same effect, at least during an early innate immunity response. Activation of later immunity elements and/or a combination of other host’s intrinsic conditions may contribute to the death of L3 in the terrestrial host. |
format | Online Article Text |
id | pubmed-9797851 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-97978512022-12-30 Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract Hrabar, Jerko Petrić, Mirela Cavallero, Serena Salvemini, Marco D’Amelio, Stefano Mladineo, Ivona Front Cell Infect Microbiol Cellular and Infection Microbiology Infective third-stage larvae (L3) of the marine nematode Anisakis pegreffii cause inflammation and clinical symptoms in humans, their accidental host, that subside and self-resolve in a couple of weeks after L3 die. To characterise the differences in an early immune response of a marine vs. terrestrial host, we stimulated peripheral blood leukocytes (PBLs) of fish (paratenic host) and rat (accidental, human-model host) with A. pegreffii crude extract and analysed PBL transcriptomes 1 and 12 h post-stimulation. Fish and rat PBLs differentially expressed 712 and 493 transcripts, respectively, between 1 and 12 h post-stimulation (false discovery rate, FDR <0.001, logFC >2). While there was a difference in the highest upregulated transcripts between two time-points, the same Gene Ontologies, biological processes (intracellular signal transduction, DNA-dependent transcription, and DNA-regulated regulation of transcription), and molecular functions (ATP and metal ion binding) were enriched in the two hosts, showing an incrementing dynamic between 1 and 12 h. This suggests that the two distinct hosts employ qualitatively different transcript cascades only to achieve the same effect, at least during an early innate immunity response. Activation of later immunity elements and/or a combination of other host’s intrinsic conditions may contribute to the death of L3 in the terrestrial host. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797851/ /pubmed/36590595 http://dx.doi.org/10.3389/fcimb.2022.1042679 Text en Copyright © 2022 Hrabar, Petrić, Cavallero, Salvemini, D’Amelio and Mladineo https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cellular and Infection Microbiology Hrabar, Jerko Petrić, Mirela Cavallero, Serena Salvemini, Marco D’Amelio, Stefano Mladineo, Ivona Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
title | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
title_full | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
title_fullStr | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
title_full_unstemmed | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
title_short | Rat and fish peripheral blood leukocytes respond distinctively to Anisakis pegreffii (Nematoda, Anisakidae) crude extract |
title_sort | rat and fish peripheral blood leukocytes respond distinctively to anisakis pegreffii (nematoda, anisakidae) crude extract |
topic | Cellular and Infection Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797851/ https://www.ncbi.nlm.nih.gov/pubmed/36590595 http://dx.doi.org/10.3389/fcimb.2022.1042679 |
work_keys_str_mv | AT hrabarjerko ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract AT petricmirela ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract AT cavalleroserena ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract AT salveminimarco ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract AT dameliostefano ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract AT mladineoivona ratandfishperipheralbloodleukocytesresponddistinctivelytoanisakispegreffiinematodaanisakidaecrudeextract |