MALDI-TOF-MS for rapid screening analysis of M-protein in serum

Monoclonal immunoglobin (M-protein) is a serum biomarker for the diagnosis of plasma cell dyscrasias. Despite limitation of analytical sensitivity and resolution, serum protein electrophoresis and immunofixation electrophoresis are still the front-line tests for the detection of M-proteins. Herein,...

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Detalles Bibliográficos
Autores principales: Li, Jie, Xu, Anping, Xie, Weijie, Li, Bowen, Yan, Cunliang, Xia, Yong, Liang, Chao, Ji, Ling
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Oncology
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797962/
https://www.ncbi.nlm.nih.gov/pubmed/36591514
http://dx.doi.org/10.3389/fonc.2022.1073479
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author Li, Jie
Xu, Anping
Xie, Weijie
Li, Bowen
Yan, Cunliang
Xia, Yong
Liang, Chao
Ji, Ling
author_facet Li, Jie
Xu, Anping
Xie, Weijie
Li, Bowen
Yan, Cunliang
Xia, Yong
Liang, Chao
Ji, Ling
author_sort Li, Jie
collection PubMed
description Monoclonal immunoglobin (M-protein) is a serum biomarker for the diagnosis of plasma cell dyscrasias. Despite limitation of analytical sensitivity and resolution, serum protein electrophoresis and immunofixation electrophoresis are still the front-line tests for the detection of M-proteins. Herein, we developed a MALDI-TOF Mass spectrometry-based method for the screening test of M-proteins in human serum. Based on the unique mass signature of different immunoglobin isotypes, M-Proteins could be rapidly identified and typed. The method demonstrated with high analytical performance and throughput, rapid and simple, which could be a new choice for the diagnosis of plasma cell dyscrasias.
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spelling pubmed-97979622022-12-30 MALDI-TOF-MS for rapid screening analysis of M-protein in serum Li, Jie Xu, Anping Xie, Weijie Li, Bowen Yan, Cunliang Xia, Yong Liang, Chao Ji, Ling Front Oncol Oncology Monoclonal immunoglobin (M-protein) is a serum biomarker for the diagnosis of plasma cell dyscrasias. Despite limitation of analytical sensitivity and resolution, serum protein electrophoresis and immunofixation electrophoresis are still the front-line tests for the detection of M-proteins. Herein, we developed a MALDI-TOF Mass spectrometry-based method for the screening test of M-proteins in human serum. Based on the unique mass signature of different immunoglobin isotypes, M-Proteins could be rapidly identified and typed. The method demonstrated with high analytical performance and throughput, rapid and simple, which could be a new choice for the diagnosis of plasma cell dyscrasias. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797962/ /pubmed/36591514 http://dx.doi.org/10.3389/fonc.2022.1073479 Text en Copyright © 2022 Li, Xu, Xie, Li, Yan, Xia, Liang and Ji https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Oncology
Li, Jie
Xu, Anping
Xie, Weijie
Li, Bowen
Yan, Cunliang
Xia, Yong
Liang, Chao
Ji, Ling
MALDI-TOF-MS for rapid screening analysis of M-protein in serum
title MALDI-TOF-MS for rapid screening analysis of M-protein in serum
title_full MALDI-TOF-MS for rapid screening analysis of M-protein in serum
title_fullStr MALDI-TOF-MS for rapid screening analysis of M-protein in serum
title_full_unstemmed MALDI-TOF-MS for rapid screening analysis of M-protein in serum
title_short MALDI-TOF-MS for rapid screening analysis of M-protein in serum
title_sort maldi-tof-ms for rapid screening analysis of m-protein in serum
topic Oncology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797962/
https://www.ncbi.nlm.nih.gov/pubmed/36591514
http://dx.doi.org/10.3389/fonc.2022.1073479
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