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Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner

Apparent contribution of complete ammonia-oxidizing organisms (comammox) to the global nitrogen cycle highlights the necessity for understanding niche differentiation of comammox bacteria among other ammonia oxidizers. While the high affinity for ammonia of the comammox species Nitrospira inopinata...

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Autores principales: Gottshall, Ekaterina Y., Godfrey, Bruce, Li, Bo, Abrahamson, Britt, Qin, Wei, Winkler, Mari
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797979/
https://www.ncbi.nlm.nih.gov/pubmed/36590435
http://dx.doi.org/10.3389/fmicb.2022.1022899
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author Gottshall, Ekaterina Y.
Godfrey, Bruce
Li, Bo
Abrahamson, Britt
Qin, Wei
Winkler, Mari
author_facet Gottshall, Ekaterina Y.
Godfrey, Bruce
Li, Bo
Abrahamson, Britt
Qin, Wei
Winkler, Mari
author_sort Gottshall, Ekaterina Y.
collection PubMed
description Apparent contribution of complete ammonia-oxidizing organisms (comammox) to the global nitrogen cycle highlights the necessity for understanding niche differentiation of comammox bacteria among other ammonia oxidizers. While the high affinity for ammonia of the comammox species Nitrospira inopinata suggests their niche partitioning is expected to be centered in oligotrophic environments, their absence in nutrient-depleted environments (such as the oceans) suggests that other (abiotic) factors might control their distribution and spatial localization within microbial communities. Many ammonia- and nitrite-oxidizing organisms are sensitive to light; however, the photosensitivity of comammox has not been explored. Since comammox bacteria encode enzymatic machinery homologous to canonical ammonia-and nitrite-oxidizers, we hypothesized that comammox N. inopinata, the only available pure culture of this group of microorganisms, may be inhibited by illumination in a similar manner. We evaluated the impact of light intensity, wavelength, and duration on the degree of photoinhibition for cultures of the comammox species N. inopinata and the soil ammonia-oxidizing archaea Nitrososphaera viennensis. Both species were highly sensitive to light. Interestingly, mimicking diurnal light exposure caused an uncoupling of ammonia and nitrite oxidation in N. inopinata, indicating nitrite oxidation might be more sensitive to light exposure than ammonia oxidation. It is likely that light influences comammox spatial distribution in natural environments such as surface fresh waters according to diurnal cycles, light attenuation coefficients, and the light penetration depths. Our findings therefore provide ecophysiological insights for further studies on comammox both in field and laboratory settings.
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spelling pubmed-97979792022-12-30 Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner Gottshall, Ekaterina Y. Godfrey, Bruce Li, Bo Abrahamson, Britt Qin, Wei Winkler, Mari Front Microbiol Microbiology Apparent contribution of complete ammonia-oxidizing organisms (comammox) to the global nitrogen cycle highlights the necessity for understanding niche differentiation of comammox bacteria among other ammonia oxidizers. While the high affinity for ammonia of the comammox species Nitrospira inopinata suggests their niche partitioning is expected to be centered in oligotrophic environments, their absence in nutrient-depleted environments (such as the oceans) suggests that other (abiotic) factors might control their distribution and spatial localization within microbial communities. Many ammonia- and nitrite-oxidizing organisms are sensitive to light; however, the photosensitivity of comammox has not been explored. Since comammox bacteria encode enzymatic machinery homologous to canonical ammonia-and nitrite-oxidizers, we hypothesized that comammox N. inopinata, the only available pure culture of this group of microorganisms, may be inhibited by illumination in a similar manner. We evaluated the impact of light intensity, wavelength, and duration on the degree of photoinhibition for cultures of the comammox species N. inopinata and the soil ammonia-oxidizing archaea Nitrososphaera viennensis. Both species were highly sensitive to light. Interestingly, mimicking diurnal light exposure caused an uncoupling of ammonia and nitrite oxidation in N. inopinata, indicating nitrite oxidation might be more sensitive to light exposure than ammonia oxidation. It is likely that light influences comammox spatial distribution in natural environments such as surface fresh waters according to diurnal cycles, light attenuation coefficients, and the light penetration depths. Our findings therefore provide ecophysiological insights for further studies on comammox both in field and laboratory settings. Frontiers Media S.A. 2022-12-15 /pmc/articles/PMC9797979/ /pubmed/36590435 http://dx.doi.org/10.3389/fmicb.2022.1022899 Text en Copyright © 2022 Gottshall, Godfrey, Li, Abrahamson, Qin and Winkler. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Gottshall, Ekaterina Y.
Godfrey, Bruce
Li, Bo
Abrahamson, Britt
Qin, Wei
Winkler, Mari
Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner
title Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner
title_full Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner
title_fullStr Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner
title_full_unstemmed Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner
title_short Photoinhibition of comammox reaction in Nitrospira inopinata in a dose- and wavelength-dependent manner
title_sort photoinhibition of comammox reaction in nitrospira inopinata in a dose- and wavelength-dependent manner
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9797979/
https://www.ncbi.nlm.nih.gov/pubmed/36590435
http://dx.doi.org/10.3389/fmicb.2022.1022899
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