Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers

[Image: see text] Liquid chromatography–mass spectrometry (LC–MS) is a major tool for the large-scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. The performance of LC is pivotal for the success of phosphoproteomics in both sensitivity and reproducibility...

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Autores principales: Duan, Xiaoxiao, Zhang, Yuanya, Huang, Xiahe, Ma, Xiao, Gao, Hui, Wang, Yingchun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2022
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798504/
https://www.ncbi.nlm.nih.gov/pubmed/36591174
http://dx.doi.org/10.1021/acsomega.2c05616
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author Duan, Xiaoxiao
Zhang, Yuanya
Huang, Xiahe
Ma, Xiao
Gao, Hui
Wang, Yingchun
author_facet Duan, Xiaoxiao
Zhang, Yuanya
Huang, Xiahe
Ma, Xiao
Gao, Hui
Wang, Yingchun
author_sort Duan, Xiaoxiao
collection PubMed
description [Image: see text] Liquid chromatography–mass spectrometry (LC–MS) is a major tool for the large-scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. The performance of LC is pivotal for the success of phosphoproteomics in both sensitivity and reproducibility. Here, we report that the widely used Easy-nLC 1200 has poor performance in analyzing phosphopeptides, particularly in terms of sensitivity and reproducibility, whereas its predecessor, Easy-nLC 1000, has a much better performance. Therefore, we suggest that Easy-nLC 1200 is not appropriate for LC–MS-based proteomics analysis for samples with a limited amount, particularly phosphopeptides from plants.
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spelling pubmed-97985042022-12-30 Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers Duan, Xiaoxiao Zhang, Yuanya Huang, Xiahe Ma, Xiao Gao, Hui Wang, Yingchun ACS Omega [Image: see text] Liquid chromatography–mass spectrometry (LC–MS) is a major tool for the large-scale qualitative and/or quantitative analysis of protein phosphorylation in cells or tissues. The performance of LC is pivotal for the success of phosphoproteomics in both sensitivity and reproducibility. Here, we report that the widely used Easy-nLC 1200 has poor performance in analyzing phosphopeptides, particularly in terms of sensitivity and reproducibility, whereas its predecessor, Easy-nLC 1000, has a much better performance. Therefore, we suggest that Easy-nLC 1200 is not appropriate for LC–MS-based proteomics analysis for samples with a limited amount, particularly phosphopeptides from plants. American Chemical Society 2022-12-14 /pmc/articles/PMC9798504/ /pubmed/36591174 http://dx.doi.org/10.1021/acsomega.2c05616 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Duan, Xiaoxiao
Zhang, Yuanya
Huang, Xiahe
Ma, Xiao
Gao, Hui
Wang, Yingchun
Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers
title Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers
title_full Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers
title_fullStr Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers
title_full_unstemmed Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers
title_short Evaluation of the Potential Risk of Analyzing Phosphopeptides with Easy-nLC 1200-Coupled Mass Spectrometers
title_sort evaluation of the potential risk of analyzing phosphopeptides with easy-nlc 1200-coupled mass spectrometers
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798504/
https://www.ncbi.nlm.nih.gov/pubmed/36591174
http://dx.doi.org/10.1021/acsomega.2c05616
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