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Titanium Membranes with Hydroxyapatite/Titania Bioactive Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing
[Image: see text] Titanium membranes and meshes are used for the repair of trauma, tumors, and hernia in dentistry and maxillofacial and abdominal surgery. But such membranes demonstrate the limited effectiveness of integration in recipients due to their bioinertness. In this study, we prepared tita...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798509/ https://www.ncbi.nlm.nih.gov/pubmed/36591210 http://dx.doi.org/10.1021/acsomega.2c05718 |
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author | Skriabin, Andrei S. Shakurov, Alexey V. Vesnin, Vladimir R. Lukina, Yulia S. Tsygankov, Petr A. Bionyshev-Abramov, Leonid L. Serejnikova, Natalya B. Vorob’ev, Evgeny V. |
author_facet | Skriabin, Andrei S. Shakurov, Alexey V. Vesnin, Vladimir R. Lukina, Yulia S. Tsygankov, Petr A. Bionyshev-Abramov, Leonid L. Serejnikova, Natalya B. Vorob’ev, Evgeny V. |
author_sort | Skriabin, Andrei S. |
collection | PubMed |
description | [Image: see text] Titanium membranes and meshes are used for the repair of trauma, tumors, and hernia in dentistry and maxillofacial and abdominal surgery. But such membranes demonstrate the limited effectiveness of integration in recipients due to their bioinertness. In this study, we prepared titania oxide (by microarc oxidation) and/or HAp (by electrophoresis deposition) coatings with alginate soaking. We used annealing at 700 °C for 2.5 h for HAp crystallinity increasing with achievement of an acceptable Ca(2+) release rate. The feedstock HAp and prepared coatings were characterized by X-ray diffraction, IR spectroscopy, electron and optical confocal microscopy, and thermal analysis, as well as the in vitro study of solubility in saline and in vivo tests with the animal model of subcutaneous implantation (with Wistar rats). Biocompatible compounds were found for all deposited coatings. We noted that the best biological response was detected for the annealed Ca–P/TiO(2) bilayer with alginate binding. In this case, the coating crystallinity was ≈40.5–50.0%. The Ca(2+) release rate was 2.042 ± 0.058%/mm(2) at 168 h after immersion in saline. Thin and mature tissue capsules with minimal inflammation and vascularization were found in histological sections. We did not detect any unwanted responses around the implants, including inflammation infiltration, suppuration, bacterial infections, tissue lyses, and, finally, implant rejection. This information is expected to be useful for understanding the properties of bioactive ceramic coatings and improving the quality of medical care in dentistry and maxillofacial surgery and other applications of titanium membranes in medicine. |
format | Online Article Text |
id | pubmed-9798509 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-97985092022-12-30 Titanium Membranes with Hydroxyapatite/Titania Bioactive Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing Skriabin, Andrei S. Shakurov, Alexey V. Vesnin, Vladimir R. Lukina, Yulia S. Tsygankov, Petr A. Bionyshev-Abramov, Leonid L. Serejnikova, Natalya B. Vorob’ev, Evgeny V. ACS Omega [Image: see text] Titanium membranes and meshes are used for the repair of trauma, tumors, and hernia in dentistry and maxillofacial and abdominal surgery. But such membranes demonstrate the limited effectiveness of integration in recipients due to their bioinertness. In this study, we prepared titania oxide (by microarc oxidation) and/or HAp (by electrophoresis deposition) coatings with alginate soaking. We used annealing at 700 °C for 2.5 h for HAp crystallinity increasing with achievement of an acceptable Ca(2+) release rate. The feedstock HAp and prepared coatings were characterized by X-ray diffraction, IR spectroscopy, electron and optical confocal microscopy, and thermal analysis, as well as the in vitro study of solubility in saline and in vivo tests with the animal model of subcutaneous implantation (with Wistar rats). Biocompatible compounds were found for all deposited coatings. We noted that the best biological response was detected for the annealed Ca–P/TiO(2) bilayer with alginate binding. In this case, the coating crystallinity was ≈40.5–50.0%. The Ca(2+) release rate was 2.042 ± 0.058%/mm(2) at 168 h after immersion in saline. Thin and mature tissue capsules with minimal inflammation and vascularization were found in histological sections. We did not detect any unwanted responses around the implants, including inflammation infiltration, suppuration, bacterial infections, tissue lyses, and, finally, implant rejection. This information is expected to be useful for understanding the properties of bioactive ceramic coatings and improving the quality of medical care in dentistry and maxillofacial surgery and other applications of titanium membranes in medicine. American Chemical Society 2022-12-12 /pmc/articles/PMC9798509/ /pubmed/36591210 http://dx.doi.org/10.1021/acsomega.2c05718 Text en © 2022 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | Skriabin, Andrei S. Shakurov, Alexey V. Vesnin, Vladimir R. Lukina, Yulia S. Tsygankov, Petr A. Bionyshev-Abramov, Leonid L. Serejnikova, Natalya B. Vorob’ev, Evgeny V. Titanium Membranes with Hydroxyapatite/Titania Bioactive Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing |
title | Titanium Membranes
with Hydroxyapatite/Titania Bioactive
Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing |
title_full | Titanium Membranes
with Hydroxyapatite/Titania Bioactive
Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing |
title_fullStr | Titanium Membranes
with Hydroxyapatite/Titania Bioactive
Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing |
title_full_unstemmed | Titanium Membranes
with Hydroxyapatite/Titania Bioactive
Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing |
title_short | Titanium Membranes
with Hydroxyapatite/Titania Bioactive
Ceramic Coatings: Characterization and In Vivo Biocompatibility Testing |
title_sort | titanium membranes
with hydroxyapatite/titania bioactive
ceramic coatings: characterization and in vivo biocompatibility testing |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798509/ https://www.ncbi.nlm.nih.gov/pubmed/36591210 http://dx.doi.org/10.1021/acsomega.2c05718 |
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