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Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses

The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has highlighted the need for vaccines that not only prevent disease but also prevent transmission. Parenteral vaccines induce robust systemic immunity but poor immunity at the respiratory mucosa. We developed a vaccine strateg...

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Autores principales: Mao, Tianyang, Israelow, Benjamin, Peña-Hernández, Mario A., Suberi, Alexandra, Zhou, Liqun, Luyten, Sophia, Reschke, Melanie, Dong, Huiping, Homer, Robert J., Saltzman, W. Mark, Iwasaki, Akiko
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798903/
https://www.ncbi.nlm.nih.gov/pubmed/36302057
http://dx.doi.org/10.1126/science.abo2523
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author Mao, Tianyang
Israelow, Benjamin
Peña-Hernández, Mario A.
Suberi, Alexandra
Zhou, Liqun
Luyten, Sophia
Reschke, Melanie
Dong, Huiping
Homer, Robert J.
Saltzman, W. Mark
Iwasaki, Akiko
author_facet Mao, Tianyang
Israelow, Benjamin
Peña-Hernández, Mario A.
Suberi, Alexandra
Zhou, Liqun
Luyten, Sophia
Reschke, Melanie
Dong, Huiping
Homer, Robert J.
Saltzman, W. Mark
Iwasaki, Akiko
author_sort Mao, Tianyang
collection PubMed
description The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has highlighted the need for vaccines that not only prevent disease but also prevent transmission. Parenteral vaccines induce robust systemic immunity but poor immunity at the respiratory mucosa. We developed a vaccine strategy that we call “prime and spike,” which leverages existing immunity generated by primary vaccination (prime) to elicit mucosal immune memory within the respiratory tract by using unadjuvanted intranasal spike boosters (spike). We show that prime and spike induces robust resident memory B and T cell responses, induces immunoglobulin A at the respiratory mucosa, boosts systemic immunity, and completely protects mice with partial immunity from lethal SARS-CoV-2 infection. Using divergent spike proteins, prime and spike enables the induction of cross-reactive immunity against sarbecoviruses.
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spelling pubmed-97989032023-01-06 Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses Mao, Tianyang Israelow, Benjamin Peña-Hernández, Mario A. Suberi, Alexandra Zhou, Liqun Luyten, Sophia Reschke, Melanie Dong, Huiping Homer, Robert J. Saltzman, W. Mark Iwasaki, Akiko Science Research Articles The severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic has highlighted the need for vaccines that not only prevent disease but also prevent transmission. Parenteral vaccines induce robust systemic immunity but poor immunity at the respiratory mucosa. We developed a vaccine strategy that we call “prime and spike,” which leverages existing immunity generated by primary vaccination (prime) to elicit mucosal immune memory within the respiratory tract by using unadjuvanted intranasal spike boosters (spike). We show that prime and spike induces robust resident memory B and T cell responses, induces immunoglobulin A at the respiratory mucosa, boosts systemic immunity, and completely protects mice with partial immunity from lethal SARS-CoV-2 infection. Using divergent spike proteins, prime and spike enables the induction of cross-reactive immunity against sarbecoviruses. American Association for the Advancement of Science 2022-11-25 /pmc/articles/PMC9798903/ /pubmed/36302057 http://dx.doi.org/10.1126/science.abo2523 Text en Copyright © 2022 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution License 4.0 (CC BY). https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution license (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Articles
Mao, Tianyang
Israelow, Benjamin
Peña-Hernández, Mario A.
Suberi, Alexandra
Zhou, Liqun
Luyten, Sophia
Reschke, Melanie
Dong, Huiping
Homer, Robert J.
Saltzman, W. Mark
Iwasaki, Akiko
Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
title Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
title_full Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
title_fullStr Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
title_full_unstemmed Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
title_short Unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
title_sort unadjuvanted intranasal spike vaccine elicits protective mucosal immunity against sarbecoviruses
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9798903/
https://www.ncbi.nlm.nih.gov/pubmed/36302057
http://dx.doi.org/10.1126/science.abo2523
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