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Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia

Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiar event to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebral venous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs....

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Autores principales: Kim, Tae-Kyun, Takeshima, Yasuhiro, Ouji, Yukiteru, Nishimura, Fumihiko, Nakagawa, Ichiro, Park, Young-Soo, Yoshikawa, Masahide, Nakase, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800262/
https://www.ncbi.nlm.nih.gov/pubmed/36590247
http://dx.doi.org/10.1016/j.ibneur.2022.12.005
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author Kim, Tae-Kyun
Takeshima, Yasuhiro
Ouji, Yukiteru
Nishimura, Fumihiko
Nakagawa, Ichiro
Park, Young-Soo
Yoshikawa, Masahide
Nakase, Hiroyuki
author_facet Kim, Tae-Kyun
Takeshima, Yasuhiro
Ouji, Yukiteru
Nishimura, Fumihiko
Nakagawa, Ichiro
Park, Young-Soo
Yoshikawa, Masahide
Nakase, Hiroyuki
author_sort Kim, Tae-Kyun
collection PubMed
description Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiar event to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebral venous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs. After creating CVI rat models via a two-vein occlusion (2-VO) method, neurogenesis was immunohistochemically evaluated by double-labeling 5-bromo-2′-deoxyuridine (BrdU)-positive cells with neuronal nuclei (NeuN) or doublecortin (DCX) antibody. Fifty Wistar rats were divided into two major groups (BrdU-NeuN and BrdU-DCX) and then separated into two subgroups (2-VO or sham). The total number of double-positive cells expressed inside a predefined region of interest (ROI) covering the ischemic area was compared between the two subgroups. Then, we divided the ROI into six sections to evaluate and compare the distribution of double-positive cells generated in each section between the two subgroups. The 2-VO subgroup presented more double-positive cells than the sham group in both BrdU-NeuN and BrdU-DCX groups, while the BrdU-DCX+2-VO group showed a characteristic distribution of double-positive cells in ROI 2 and ROI 3, suggesting areas of the ischemic core and penumbra, with a significant difference compared to the BrdU-DCX+sham group. This study demonstrates that CVI has the potential to induce endogenous neurogenesis, with significant numbers of both newly generated neurons and precursors observed in the ischemic area. The distribution of these cells suggests that the cortex could be the main origin of neurogenesis after cortical CVI.
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spelling pubmed-98002622022-12-31 Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia Kim, Tae-Kyun Takeshima, Yasuhiro Ouji, Yukiteru Nishimura, Fumihiko Nakagawa, Ichiro Park, Young-Soo Yoshikawa, Masahide Nakase, Hiroyuki IBRO Neurosci Rep Research Paper Neurogenesis in the subventricular zone (SVZ), subgranular zone (SGZ), and cerebral cortex is now a familiar event to confirm by cerebral arterial ischemia in rat models. However, it remains unclear whether cerebral venous ischemia (CVI) alone causes neurogenesis, and where that neurogenesis occurs. After creating CVI rat models via a two-vein occlusion (2-VO) method, neurogenesis was immunohistochemically evaluated by double-labeling 5-bromo-2′-deoxyuridine (BrdU)-positive cells with neuronal nuclei (NeuN) or doublecortin (DCX) antibody. Fifty Wistar rats were divided into two major groups (BrdU-NeuN and BrdU-DCX) and then separated into two subgroups (2-VO or sham). The total number of double-positive cells expressed inside a predefined region of interest (ROI) covering the ischemic area was compared between the two subgroups. Then, we divided the ROI into six sections to evaluate and compare the distribution of double-positive cells generated in each section between the two subgroups. The 2-VO subgroup presented more double-positive cells than the sham group in both BrdU-NeuN and BrdU-DCX groups, while the BrdU-DCX+2-VO group showed a characteristic distribution of double-positive cells in ROI 2 and ROI 3, suggesting areas of the ischemic core and penumbra, with a significant difference compared to the BrdU-DCX+sham group. This study demonstrates that CVI has the potential to induce endogenous neurogenesis, with significant numbers of both newly generated neurons and precursors observed in the ischemic area. The distribution of these cells suggests that the cortex could be the main origin of neurogenesis after cortical CVI. Elsevier 2022-12-13 /pmc/articles/PMC9800262/ /pubmed/36590247 http://dx.doi.org/10.1016/j.ibneur.2022.12.005 Text en © 2022 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Paper
Kim, Tae-Kyun
Takeshima, Yasuhiro
Ouji, Yukiteru
Nishimura, Fumihiko
Nakagawa, Ichiro
Park, Young-Soo
Yoshikawa, Masahide
Nakase, Hiroyuki
Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
title Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
title_full Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
title_fullStr Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
title_full_unstemmed Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
title_short Expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
title_sort expression and distribution of generated neurons and endogenous precursors in rat cerebral cortical venous ischemia
topic Research Paper
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800262/
https://www.ncbi.nlm.nih.gov/pubmed/36590247
http://dx.doi.org/10.1016/j.ibneur.2022.12.005
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