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The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains

Infection with Thelazia nematodes results in eye disease in wild and domestic animals. The aim of the present study was to describe the occurrence of Thelazia nematodes in European bison, and to subject the isolated parasites to molecular identification and phylogenetical analysis. The eyeballs of 1...

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Autores principales: Filip-Hutsch, Katarzyna, Laskowski, Zdzisław, Myczka, Anna W., Czopowicz, Michał, Moskwa, Bożena, Demiaszkiewicz, Aleksander W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800370/
https://www.ncbi.nlm.nih.gov/pubmed/36581768
http://dx.doi.org/10.1038/s41598-022-27191-x
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author Filip-Hutsch, Katarzyna
Laskowski, Zdzisław
Myczka, Anna W.
Czopowicz, Michał
Moskwa, Bożena
Demiaszkiewicz, Aleksander W.
author_facet Filip-Hutsch, Katarzyna
Laskowski, Zdzisław
Myczka, Anna W.
Czopowicz, Michał
Moskwa, Bożena
Demiaszkiewicz, Aleksander W.
author_sort Filip-Hutsch, Katarzyna
collection PubMed
description Infection with Thelazia nematodes results in eye disease in wild and domestic animals. The aim of the present study was to describe the occurrence of Thelazia nematodes in European bison, and to subject the isolated parasites to molecular identification and phylogenetical analysis. The eyeballs of 18 European bison from the Bieszczady Mountains, culled due to dysfunctional vision, were collected for study. The conjunctival sacs, tear ducts, corneal surface and nictitating membrane were rinsed with a saline solution. Any obtained nematodes were isolated under a stereoscopic microscope, and then identified as T. gulosa or T. skrjabini by molecular analysis of partial cox1 sequences. The prevalence of infection with Thelazia spp. was found to be 61%, with a 95% confidence interval (CI 95%) of 39–80%. Thelazia skrjabini was isolated from 56% (CI 95% 34–75%) of examined animals; T. gulosa was significantly less common (p = 0.038) with the prevalence of infection reaching 22% (CI 95% 9–45%). Three European bison were cross-infected with both T. gulosa and T. skrjabini. Phylogenetic analysis found the obtained sequences to be similar to those of Thelazia species from domestic ungulates in Europe. Infection intensity ranged from 1 to 16 nematodes per individual (median of three nematodes), and was significantly higher in females (6 nematodes) than in males (1 nematode; p = 0.019). A tendency for seasonal occurrence of nematodes in European bison was also observed. Our study provides further information regarding the patterns of Thelazia transmission in European bison in Poland.
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spelling pubmed-98003702022-12-31 The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains Filip-Hutsch, Katarzyna Laskowski, Zdzisław Myczka, Anna W. Czopowicz, Michał Moskwa, Bożena Demiaszkiewicz, Aleksander W. Sci Rep Article Infection with Thelazia nematodes results in eye disease in wild and domestic animals. The aim of the present study was to describe the occurrence of Thelazia nematodes in European bison, and to subject the isolated parasites to molecular identification and phylogenetical analysis. The eyeballs of 18 European bison from the Bieszczady Mountains, culled due to dysfunctional vision, were collected for study. The conjunctival sacs, tear ducts, corneal surface and nictitating membrane were rinsed with a saline solution. Any obtained nematodes were isolated under a stereoscopic microscope, and then identified as T. gulosa or T. skrjabini by molecular analysis of partial cox1 sequences. The prevalence of infection with Thelazia spp. was found to be 61%, with a 95% confidence interval (CI 95%) of 39–80%. Thelazia skrjabini was isolated from 56% (CI 95% 34–75%) of examined animals; T. gulosa was significantly less common (p = 0.038) with the prevalence of infection reaching 22% (CI 95% 9–45%). Three European bison were cross-infected with both T. gulosa and T. skrjabini. Phylogenetic analysis found the obtained sequences to be similar to those of Thelazia species from domestic ungulates in Europe. Infection intensity ranged from 1 to 16 nematodes per individual (median of three nematodes), and was significantly higher in females (6 nematodes) than in males (1 nematode; p = 0.019). A tendency for seasonal occurrence of nematodes in European bison was also observed. Our study provides further information regarding the patterns of Thelazia transmission in European bison in Poland. Nature Publishing Group UK 2022-12-29 /pmc/articles/PMC9800370/ /pubmed/36581768 http://dx.doi.org/10.1038/s41598-022-27191-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Filip-Hutsch, Katarzyna
Laskowski, Zdzisław
Myczka, Anna W.
Czopowicz, Michał
Moskwa, Bożena
Demiaszkiewicz, Aleksander W.
The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains
title The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains
title_full The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains
title_fullStr The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains
title_full_unstemmed The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains
title_short The occurrence and molecular identification of Thelazia spp. in European bison (Bison bonasus) in the Bieszczady Mountains
title_sort occurrence and molecular identification of thelazia spp. in european bison (bison bonasus) in the bieszczady mountains
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800370/
https://www.ncbi.nlm.nih.gov/pubmed/36581768
http://dx.doi.org/10.1038/s41598-022-27191-x
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