Cargando…
Detrimental effects of simulated microgravity on mast cell homeostasis and function
Exposure to microgravity causes significant alterations in astronauts’ immune systems during spaceflight; however, it is unknown whether microgravity affects mast cell homeostasis and activation. Here we show that microgravity negatively regulates the survival and effector function of mast cells. Mu...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800517/ https://www.ncbi.nlm.nih.gov/pubmed/36591304 http://dx.doi.org/10.3389/fimmu.2022.1055531 |
_version_ | 1784861306862436352 |
---|---|
author | Kim, Minjin Jang, Gyeongin Kim, Kyu-Sung Shin, Jinwook |
author_facet | Kim, Minjin Jang, Gyeongin Kim, Kyu-Sung Shin, Jinwook |
author_sort | Kim, Minjin |
collection | PubMed |
description | Exposure to microgravity causes significant alterations in astronauts’ immune systems during spaceflight; however, it is unknown whether microgravity affects mast cell homeostasis and activation. Here we show that microgravity negatively regulates the survival and effector function of mast cells. Murine bone marrow-derived mast cells (BMMCs) were cultured with IL-3 in a rotary cell culture system (RCCS) that generates a simulated microgravity (SMG) environment. BMMCs exposed to SMG showed enhanced apoptosis along with the downregulation of Bcl-2, and reduced proliferation compared to Earth’s gravity (1G) controls. The reduction in survival and proliferation caused by SMG exposure was recovered by stem cell factor. In addition, SMG impaired mast cell degranulation and cytokine secretion. BMMCs pre-exposed to SMG showed decreased release of β-hexosaminidase, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) upon stimulation with phorbol 12-myristate-13-acetate (PMA) plus calcium ionophore ionomycin, which correlated with decreased calcium influx. These findings provide new insights into microgravity-mediated alterations of mast cell phenotypes, contributing to the understanding of immune system dysfunction for further space medicine research. |
format | Online Article Text |
id | pubmed-9800517 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98005172022-12-31 Detrimental effects of simulated microgravity on mast cell homeostasis and function Kim, Minjin Jang, Gyeongin Kim, Kyu-Sung Shin, Jinwook Front Immunol Immunology Exposure to microgravity causes significant alterations in astronauts’ immune systems during spaceflight; however, it is unknown whether microgravity affects mast cell homeostasis and activation. Here we show that microgravity negatively regulates the survival and effector function of mast cells. Murine bone marrow-derived mast cells (BMMCs) were cultured with IL-3 in a rotary cell culture system (RCCS) that generates a simulated microgravity (SMG) environment. BMMCs exposed to SMG showed enhanced apoptosis along with the downregulation of Bcl-2, and reduced proliferation compared to Earth’s gravity (1G) controls. The reduction in survival and proliferation caused by SMG exposure was recovered by stem cell factor. In addition, SMG impaired mast cell degranulation and cytokine secretion. BMMCs pre-exposed to SMG showed decreased release of β-hexosaminidase, interleukin-6 (IL-6), and tumor necrosis factor-α (TNF-α) upon stimulation with phorbol 12-myristate-13-acetate (PMA) plus calcium ionophore ionomycin, which correlated with decreased calcium influx. These findings provide new insights into microgravity-mediated alterations of mast cell phenotypes, contributing to the understanding of immune system dysfunction for further space medicine research. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800517/ /pubmed/36591304 http://dx.doi.org/10.3389/fimmu.2022.1055531 Text en Copyright © 2022 Kim, Jang, Kim and Shin https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Kim, Minjin Jang, Gyeongin Kim, Kyu-Sung Shin, Jinwook Detrimental effects of simulated microgravity on mast cell homeostasis and function |
title | Detrimental effects of simulated microgravity on mast cell homeostasis and function |
title_full | Detrimental effects of simulated microgravity on mast cell homeostasis and function |
title_fullStr | Detrimental effects of simulated microgravity on mast cell homeostasis and function |
title_full_unstemmed | Detrimental effects of simulated microgravity on mast cell homeostasis and function |
title_short | Detrimental effects of simulated microgravity on mast cell homeostasis and function |
title_sort | detrimental effects of simulated microgravity on mast cell homeostasis and function |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800517/ https://www.ncbi.nlm.nih.gov/pubmed/36591304 http://dx.doi.org/10.3389/fimmu.2022.1055531 |
work_keys_str_mv | AT kimminjin detrimentaleffectsofsimulatedmicrogravityonmastcellhomeostasisandfunction AT janggyeongin detrimentaleffectsofsimulatedmicrogravityonmastcellhomeostasisandfunction AT kimkyusung detrimentaleffectsofsimulatedmicrogravityonmastcellhomeostasisandfunction AT shinjinwook detrimentaleffectsofsimulatedmicrogravityonmastcellhomeostasisandfunction |