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Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors

Establishing metabolic programming begins during fetal and postnatal development, and early-life lipid exposures play a critical role during neonatal adipogenesis. We define how neonatal consumption of a low omega-6 to −3 fatty acid ratio (n6/n3 FA ratio) establishes FA oxidation in adipocyte precur...

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Autores principales: Varshney, Rohan, Das, Snehasis, Trahan, G. Devon, Farriester, Jacob W., Mullen, Gregory P., Kyere-Davies, Gertrude, Presby, David M., Houck, Julie A., Webb, Patricia G., Dzieciatkowska, Monika, Jones, Kenneth L., Rodeheffer, Matthew S., Friedman, Jacob E., MacLean, Paul S., Rudolph, Michael C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800552/
https://www.ncbi.nlm.nih.gov/pubmed/36590177
http://dx.doi.org/10.1016/j.isci.2022.105750
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author Varshney, Rohan
Das, Snehasis
Trahan, G. Devon
Farriester, Jacob W.
Mullen, Gregory P.
Kyere-Davies, Gertrude
Presby, David M.
Houck, Julie A.
Webb, Patricia G.
Dzieciatkowska, Monika
Jones, Kenneth L.
Rodeheffer, Matthew S.
Friedman, Jacob E.
MacLean, Paul S.
Rudolph, Michael C.
author_facet Varshney, Rohan
Das, Snehasis
Trahan, G. Devon
Farriester, Jacob W.
Mullen, Gregory P.
Kyere-Davies, Gertrude
Presby, David M.
Houck, Julie A.
Webb, Patricia G.
Dzieciatkowska, Monika
Jones, Kenneth L.
Rodeheffer, Matthew S.
Friedman, Jacob E.
MacLean, Paul S.
Rudolph, Michael C.
author_sort Varshney, Rohan
collection PubMed
description Establishing metabolic programming begins during fetal and postnatal development, and early-life lipid exposures play a critical role during neonatal adipogenesis. We define how neonatal consumption of a low omega-6 to −3 fatty acid ratio (n6/n3 FA ratio) establishes FA oxidation in adipocyte precursor cells (APCs) before they become adipocytes. In vivo, APCs isolated from mouse pups exposed to the low n6/n3 FA ratio had superior FA oxidation capacity, elevated beige adipocyte mRNAs Ppargc1α, Ucp2, and Runx1, and increased nuclear receptor NR2F2 protein. In vitro, APC treatment with NR2F2 ligand-induced beige adipocyte mRNAs and increased mitochondrial potential but not mass. Single-cell RNA-sequencing analysis revealed low n6/n3 FA ratio yielded more mitochondrial-high APCs and linked APC NR2F2 levels with beige adipocyte signatures and FA oxidation. Establishing beige adipogenesis is of clinical relevance, because fat depots with energetically active, smaller, and more numerous adipocytes improve metabolism and delay metabolic dysfunction.
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spelling pubmed-98005522022-12-31 Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors Varshney, Rohan Das, Snehasis Trahan, G. Devon Farriester, Jacob W. Mullen, Gregory P. Kyere-Davies, Gertrude Presby, David M. Houck, Julie A. Webb, Patricia G. Dzieciatkowska, Monika Jones, Kenneth L. Rodeheffer, Matthew S. Friedman, Jacob E. MacLean, Paul S. Rudolph, Michael C. iScience Article Establishing metabolic programming begins during fetal and postnatal development, and early-life lipid exposures play a critical role during neonatal adipogenesis. We define how neonatal consumption of a low omega-6 to −3 fatty acid ratio (n6/n3 FA ratio) establishes FA oxidation in adipocyte precursor cells (APCs) before they become adipocytes. In vivo, APCs isolated from mouse pups exposed to the low n6/n3 FA ratio had superior FA oxidation capacity, elevated beige adipocyte mRNAs Ppargc1α, Ucp2, and Runx1, and increased nuclear receptor NR2F2 protein. In vitro, APC treatment with NR2F2 ligand-induced beige adipocyte mRNAs and increased mitochondrial potential but not mass. Single-cell RNA-sequencing analysis revealed low n6/n3 FA ratio yielded more mitochondrial-high APCs and linked APC NR2F2 levels with beige adipocyte signatures and FA oxidation. Establishing beige adipogenesis is of clinical relevance, because fat depots with energetically active, smaller, and more numerous adipocytes improve metabolism and delay metabolic dysfunction. Elsevier 2022-12-06 /pmc/articles/PMC9800552/ /pubmed/36590177 http://dx.doi.org/10.1016/j.isci.2022.105750 Text en https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Article
Varshney, Rohan
Das, Snehasis
Trahan, G. Devon
Farriester, Jacob W.
Mullen, Gregory P.
Kyere-Davies, Gertrude
Presby, David M.
Houck, Julie A.
Webb, Patricia G.
Dzieciatkowska, Monika
Jones, Kenneth L.
Rodeheffer, Matthew S.
Friedman, Jacob E.
MacLean, Paul S.
Rudolph, Michael C.
Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
title Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
title_full Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
title_fullStr Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
title_full_unstemmed Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
title_short Neonatal intake of Omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
title_sort neonatal intake of omega-3 fatty acids enhances lipid oxidation in adipocyte precursors
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800552/
https://www.ncbi.nlm.nih.gov/pubmed/36590177
http://dx.doi.org/10.1016/j.isci.2022.105750
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