Cargando…

Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?

A wide range of microorganisms inhabit biocrusts of arctic and sub-arctic regions. These taxa live and thrive under extreme conditions and, moreover, play important roles in biogeochemical cycling. Nevertheless, their diversity and abundance remain ambiguous. Here, we studied microbial community com...

Descripción completa

Detalles Bibliográficos
Autores principales: Pushkareva, Ekaterina, Elster, Josef, Holzinger, Andreas, Niedzwiedz, Sarina, Becker, Burkhard
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800606/
https://www.ncbi.nlm.nih.gov/pubmed/36590427
http://dx.doi.org/10.3389/fmicb.2022.1048522
_version_ 1784861326648016896
author Pushkareva, Ekaterina
Elster, Josef
Holzinger, Andreas
Niedzwiedz, Sarina
Becker, Burkhard
author_facet Pushkareva, Ekaterina
Elster, Josef
Holzinger, Andreas
Niedzwiedz, Sarina
Becker, Burkhard
author_sort Pushkareva, Ekaterina
collection PubMed
description A wide range of microorganisms inhabit biocrusts of arctic and sub-arctic regions. These taxa live and thrive under extreme conditions and, moreover, play important roles in biogeochemical cycling. Nevertheless, their diversity and abundance remain ambiguous. Here, we studied microbial community composition in biocrusts from Svalbard and Iceland using amplicon sequencing and epifluorescence microscopy. Sequencing of 16S rRNA gene revealed the dominance of Chloroflexi in the biocrusts from Iceland and Longyearbyen, and Acidobacteria in the biocrusts from Ny-Ålesund and South Svalbard. Within the 18S rRNA gene sequencing dataset, Chloroplastida prevailed in all the samples with dominance of Trebouxiophyceae in the biocrusts from Ny-Ålesund and Embryophyta in the biocrusts from the other localities. Furthermore, cyanobacterial number of cells and biovolume exceeded the microalgal in the biocrusts. Community compositions in the studied sites were correlated to the measured chemical parameters such as conductivity, pH, soil organic matter and mineral nitrogen contents. In addition, co-occurrence analysis showed the dominance of positive potential interactions and, bacterial and eukaryotic taxa co-occurred more frequently together.
format Online
Article
Text
id pubmed-9800606
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98006062022-12-31 Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially? Pushkareva, Ekaterina Elster, Josef Holzinger, Andreas Niedzwiedz, Sarina Becker, Burkhard Front Microbiol Microbiology A wide range of microorganisms inhabit biocrusts of arctic and sub-arctic regions. These taxa live and thrive under extreme conditions and, moreover, play important roles in biogeochemical cycling. Nevertheless, their diversity and abundance remain ambiguous. Here, we studied microbial community composition in biocrusts from Svalbard and Iceland using amplicon sequencing and epifluorescence microscopy. Sequencing of 16S rRNA gene revealed the dominance of Chloroflexi in the biocrusts from Iceland and Longyearbyen, and Acidobacteria in the biocrusts from Ny-Ålesund and South Svalbard. Within the 18S rRNA gene sequencing dataset, Chloroplastida prevailed in all the samples with dominance of Trebouxiophyceae in the biocrusts from Ny-Ålesund and Embryophyta in the biocrusts from the other localities. Furthermore, cyanobacterial number of cells and biovolume exceeded the microalgal in the biocrusts. Community compositions in the studied sites were correlated to the measured chemical parameters such as conductivity, pH, soil organic matter and mineral nitrogen contents. In addition, co-occurrence analysis showed the dominance of positive potential interactions and, bacterial and eukaryotic taxa co-occurred more frequently together. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800606/ /pubmed/36590427 http://dx.doi.org/10.3389/fmicb.2022.1048522 Text en Copyright © 2022 Pushkareva, Elster, Holzinger, Niedzwiedz and Becker. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Pushkareva, Ekaterina
Elster, Josef
Holzinger, Andreas
Niedzwiedz, Sarina
Becker, Burkhard
Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
title Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
title_full Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
title_fullStr Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
title_full_unstemmed Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
title_short Biocrusts from Iceland and Svalbard: Does microbial community composition differ substantially?
title_sort biocrusts from iceland and svalbard: does microbial community composition differ substantially?
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800606/
https://www.ncbi.nlm.nih.gov/pubmed/36590427
http://dx.doi.org/10.3389/fmicb.2022.1048522
work_keys_str_mv AT pushkarevaekaterina biocrustsfromicelandandsvalbarddoesmicrobialcommunitycompositiondiffersubstantially
AT elsterjosef biocrustsfromicelandandsvalbarddoesmicrobialcommunitycompositiondiffersubstantially
AT holzingerandreas biocrustsfromicelandandsvalbarddoesmicrobialcommunitycompositiondiffersubstantially
AT niedzwiedzsarina biocrustsfromicelandandsvalbarddoesmicrobialcommunitycompositiondiffersubstantially
AT beckerburkhard biocrustsfromicelandandsvalbarddoesmicrobialcommunitycompositiondiffersubstantially