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Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil
INTRODUCTION: Soil ammonia oxidation, which acts as the first and rate-limiting step of nitrification, is driven by ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and complete ammonia oxidizer (comammox, amoA gene of clade-A and clade-B). Straw returning, widely used ecological te...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800607/ https://www.ncbi.nlm.nih.gov/pubmed/36590424 http://dx.doi.org/10.3389/fmicb.2022.1069554 |
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author | Chu, Xiangqian Bai, Naling Zheng, Xianqing Wang, Quanhua Pan, Xi Li, Shuangxi Zhang, Juanqin Zhang, Haiyun He, Wenjie Zhong, Feng Lv, Weiguang Zhang, Hanlin |
author_facet | Chu, Xiangqian Bai, Naling Zheng, Xianqing Wang, Quanhua Pan, Xi Li, Shuangxi Zhang, Juanqin Zhang, Haiyun He, Wenjie Zhong, Feng Lv, Weiguang Zhang, Hanlin |
author_sort | Chu, Xiangqian |
collection | PubMed |
description | INTRODUCTION: Soil ammonia oxidation, which acts as the first and rate-limiting step of nitrification, is driven by ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and complete ammonia oxidizer (comammox, amoA gene of clade-A and clade-B). Straw returning, widely used ecological technology in China, is an effective measure for promoting straw decomposition and soil nutrient cycling when combined with earthworm addition. However, the effects of straw returning combined with earthworm addition on soil ammonia oxidizers remain poorly understood. METHODS: A 2-year plot experiment was conducted with 5 treatments: no fertilizer (CK); regular fertilization (RT); straw returning (SR); earthworm addition (W); straw returning + earthworm addition (SRW). The AOA, AOB, comammox clade-A and clade-B community microbial diversities and structures were investigated by high-throughput sequencing. RESULTS: The results showed that (1) compared to RT treatment, W, SR, and SRW treatments all significantly increased the richness of AOA and comammox clade-A and clade-B (p < 0.05), and the richness of AOB was only significantly promoted by SRW treatment (p < 0.05). However, only SRW had a higher comammox clade-B diversity index than RT. (2) The ammonia oxidizer community structures were altered by both straw returning and earthworm addition. Soil NH(4)(+)-N was the critical environmental driver for altering the ammonia oxidizer community structure. (3) Compared with RT treatment, the soil potential nitrification rate (PNR) of W and SRW treatments increased by 1.19 and 1.20 times, respectively. The PNR was significantly positively correlated with AOB abundance (path coefficient = 0.712, p < 0.05) and negatively correlated with clade-B abundance (path coefficient = −0.106, p < 0.05). DISCUSSION: This study provides scientific support for the application of straw returning combined with earthworm addition to improve soil nitrification with respect to soil ammonia-oxidizing microorganisms. |
format | Online Article Text |
id | pubmed-9800607 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98006072022-12-31 Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil Chu, Xiangqian Bai, Naling Zheng, Xianqing Wang, Quanhua Pan, Xi Li, Shuangxi Zhang, Juanqin Zhang, Haiyun He, Wenjie Zhong, Feng Lv, Weiguang Zhang, Hanlin Front Microbiol Microbiology INTRODUCTION: Soil ammonia oxidation, which acts as the first and rate-limiting step of nitrification, is driven by ammonia-oxidizing bacteria (AOB), ammonia-oxidizing archaea (AOA) and complete ammonia oxidizer (comammox, amoA gene of clade-A and clade-B). Straw returning, widely used ecological technology in China, is an effective measure for promoting straw decomposition and soil nutrient cycling when combined with earthworm addition. However, the effects of straw returning combined with earthworm addition on soil ammonia oxidizers remain poorly understood. METHODS: A 2-year plot experiment was conducted with 5 treatments: no fertilizer (CK); regular fertilization (RT); straw returning (SR); earthworm addition (W); straw returning + earthworm addition (SRW). The AOA, AOB, comammox clade-A and clade-B community microbial diversities and structures were investigated by high-throughput sequencing. RESULTS: The results showed that (1) compared to RT treatment, W, SR, and SRW treatments all significantly increased the richness of AOA and comammox clade-A and clade-B (p < 0.05), and the richness of AOB was only significantly promoted by SRW treatment (p < 0.05). However, only SRW had a higher comammox clade-B diversity index than RT. (2) The ammonia oxidizer community structures were altered by both straw returning and earthworm addition. Soil NH(4)(+)-N was the critical environmental driver for altering the ammonia oxidizer community structure. (3) Compared with RT treatment, the soil potential nitrification rate (PNR) of W and SRW treatments increased by 1.19 and 1.20 times, respectively. The PNR was significantly positively correlated with AOB abundance (path coefficient = 0.712, p < 0.05) and negatively correlated with clade-B abundance (path coefficient = −0.106, p < 0.05). DISCUSSION: This study provides scientific support for the application of straw returning combined with earthworm addition to improve soil nitrification with respect to soil ammonia-oxidizing microorganisms. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800607/ /pubmed/36590424 http://dx.doi.org/10.3389/fmicb.2022.1069554 Text en Copyright © 2022 Chu, Bai, Zheng, Wang, Pan, Li, Zhang, Zhang, He, Zhong, Lv and Zhang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Microbiology Chu, Xiangqian Bai, Naling Zheng, Xianqing Wang, Quanhua Pan, Xi Li, Shuangxi Zhang, Juanqin Zhang, Haiyun He, Wenjie Zhong, Feng Lv, Weiguang Zhang, Hanlin Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
title | Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
title_full | Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
title_fullStr | Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
title_full_unstemmed | Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
title_short | Effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
title_sort | effects of straw returning combined with earthworm addition on nitrification and ammonia oxidizers in paddy soil |
topic | Microbiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800607/ https://www.ncbi.nlm.nih.gov/pubmed/36590424 http://dx.doi.org/10.3389/fmicb.2022.1069554 |
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