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Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling

Elderly was the most affected population during the first COVID-19 and those living in nursing homes represented the most vulnerable group, with high mortality rates, until vaccines became available. In a previous article, we presented an open-label trial showing the beneficial effect of the strain...

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Autores principales: Mozota, Marta, Castro, Irma, Gómez-Torres, Natalia, Arroyo, Rebeca, Gutiérrez-Díaz, Isabel, Delgado, Susana, Rodríguez, Juan Miguel, Alba, Claudio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800801/
https://www.ncbi.nlm.nih.gov/pubmed/36590434
http://dx.doi.org/10.3389/fmicb.2022.1052675
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author Mozota, Marta
Castro, Irma
Gómez-Torres, Natalia
Arroyo, Rebeca
Gutiérrez-Díaz, Isabel
Delgado, Susana
Rodríguez, Juan Miguel
Alba, Claudio
author_facet Mozota, Marta
Castro, Irma
Gómez-Torres, Natalia
Arroyo, Rebeca
Gutiérrez-Díaz, Isabel
Delgado, Susana
Rodríguez, Juan Miguel
Alba, Claudio
author_sort Mozota, Marta
collection PubMed
description Elderly was the most affected population during the first COVID-19 and those living in nursing homes represented the most vulnerable group, with high mortality rates, until vaccines became available. In a previous article, we presented an open-label trial showing the beneficial effect of the strain Ligilactobacillus salivarius CECT 30632 (previously known as L. salivarius MP101) on the functional and nutritional status, and on the nasal and fecal inflammatory profiles of elderly residing in a nursing home highly affected by the pandemic. The objective of this post-hoc analysis was to elucidate if there were changes in the nasal and fecal bacteriomes of a subset of these patients as a result of the administration of the strain for 4 months and, also, its impact on their fecal fatty acids profiles. Culture-based methods showed that, while L. salivarius (species level) could not be detected in any of the fecal samples at day 0, L. salivarius CECT 30632 (strain level) was present in all the recruited people at day 120. Paradoxically, the increase in the L. salivarius counts was not reflected in changes in the metataxonomic analysis of the nasal and fecal samples or in changes in the fatty acid profiles in the fecal samples of the recruited people. Overall, our results indicate that L. salivarius CECT 30632 colonized, at least temporarily, the intestinal tract of the recruited elderly and may have contributed to improvements in their functional, nutritional, and immunological status, without changing the general structure of their nasal and fecal bacteriomes when assessed at the genus level. They also suggest the ability of low abundance bacteria to train immunity.
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spelling pubmed-98008012022-12-31 Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling Mozota, Marta Castro, Irma Gómez-Torres, Natalia Arroyo, Rebeca Gutiérrez-Díaz, Isabel Delgado, Susana Rodríguez, Juan Miguel Alba, Claudio Front Microbiol Microbiology Elderly was the most affected population during the first COVID-19 and those living in nursing homes represented the most vulnerable group, with high mortality rates, until vaccines became available. In a previous article, we presented an open-label trial showing the beneficial effect of the strain Ligilactobacillus salivarius CECT 30632 (previously known as L. salivarius MP101) on the functional and nutritional status, and on the nasal and fecal inflammatory profiles of elderly residing in a nursing home highly affected by the pandemic. The objective of this post-hoc analysis was to elucidate if there were changes in the nasal and fecal bacteriomes of a subset of these patients as a result of the administration of the strain for 4 months and, also, its impact on their fecal fatty acids profiles. Culture-based methods showed that, while L. salivarius (species level) could not be detected in any of the fecal samples at day 0, L. salivarius CECT 30632 (strain level) was present in all the recruited people at day 120. Paradoxically, the increase in the L. salivarius counts was not reflected in changes in the metataxonomic analysis of the nasal and fecal samples or in changes in the fatty acid profiles in the fecal samples of the recruited people. Overall, our results indicate that L. salivarius CECT 30632 colonized, at least temporarily, the intestinal tract of the recruited elderly and may have contributed to improvements in their functional, nutritional, and immunological status, without changing the general structure of their nasal and fecal bacteriomes when assessed at the genus level. They also suggest the ability of low abundance bacteria to train immunity. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800801/ /pubmed/36590434 http://dx.doi.org/10.3389/fmicb.2022.1052675 Text en Copyright © 2022 Mozota, Castro, Gómez-Torres, Arroyo, Gutiérrez-Díaz, Delgado, Rodríguez and Alba. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Microbiology
Mozota, Marta
Castro, Irma
Gómez-Torres, Natalia
Arroyo, Rebeca
Gutiérrez-Díaz, Isabel
Delgado, Susana
Rodríguez, Juan Miguel
Alba, Claudio
Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling
title Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling
title_full Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling
title_fullStr Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling
title_full_unstemmed Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling
title_short Administration of Ligilactobacillus salivarius CECT 30632 to elderly during the COVID-19 pandemic: Nasal and fecal metataxonomic analysis and fatty acid profiling
title_sort administration of ligilactobacillus salivarius cect 30632 to elderly during the covid-19 pandemic: nasal and fecal metataxonomic analysis and fatty acid profiling
topic Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800801/
https://www.ncbi.nlm.nih.gov/pubmed/36590434
http://dx.doi.org/10.3389/fmicb.2022.1052675
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