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Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation d...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800929/ https://www.ncbi.nlm.nih.gov/pubmed/36589077 http://dx.doi.org/10.3389/fpls.2022.1052463 |
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author | Lu, Lu Wu, Xinru Tang, Yao Zhu, Liming Hao, Zhaodong Zhang, Jingbo Li, Xinle Shi, Jisen Chen, Jinhui Cheng, Tielong |
author_facet | Lu, Lu Wu, Xinru Tang, Yao Zhu, Liming Hao, Zhaodong Zhang, Jingbo Li, Xinle Shi, Jisen Chen, Jinhui Cheng, Tielong |
author_sort | Lu, Lu |
collection | PubMed |
description | The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation during abiotic stresses, which inhibits the potential application of these genes to improve environmental adaptability of glycophytes. In this study, we constructed Nitraria billardieri CIPK25 overexpressing Arabidopsis and analyzed the seedling development under salt treatment. Our results show that Arabidopsis with NbCIPK25 expression exhibits more vigorous growth than wild type plants under salt condition. To gain insight into the molecular mechanisms underlying salt tolerance, we profiled the transcriptome of WT and transgenic plants via RNA-seq. GO and KEGG analyses revealed that upregulated genes in NbCIPK25 overexpressing seedlings under salt stress are enriched in photosynthesis related terms; Calvin-cycle genes including glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) are significantly upregulated in transgenic plants, which is consistent with a decreased level of NADPH (GAPDH substrate) and increased level of NADP(+). Accordingly, NbCIPK25 overexpressing plants exhibited more efficient photosynthesis; soluble sugar and proteins, as photosynthesis products, showed a higher accumulation in transgenic plants. These results provide molecular insight into how NbCIPK25 promotes the expression of genes involved in photosynthesis, thereby maintaining plant growth under salt stress. Our finding supports the potential application of halophyte-derived NbCIPK25 in genetic modification for better salt adaptation. |
format | Online Article Text |
id | pubmed-9800929 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98009292022-12-31 Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress Lu, Lu Wu, Xinru Tang, Yao Zhu, Liming Hao, Zhaodong Zhang, Jingbo Li, Xinle Shi, Jisen Chen, Jinhui Cheng, Tielong Front Plant Sci Plant Science The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation during abiotic stresses, which inhibits the potential application of these genes to improve environmental adaptability of glycophytes. In this study, we constructed Nitraria billardieri CIPK25 overexpressing Arabidopsis and analyzed the seedling development under salt treatment. Our results show that Arabidopsis with NbCIPK25 expression exhibits more vigorous growth than wild type plants under salt condition. To gain insight into the molecular mechanisms underlying salt tolerance, we profiled the transcriptome of WT and transgenic plants via RNA-seq. GO and KEGG analyses revealed that upregulated genes in NbCIPK25 overexpressing seedlings under salt stress are enriched in photosynthesis related terms; Calvin-cycle genes including glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) are significantly upregulated in transgenic plants, which is consistent with a decreased level of NADPH (GAPDH substrate) and increased level of NADP(+). Accordingly, NbCIPK25 overexpressing plants exhibited more efficient photosynthesis; soluble sugar and proteins, as photosynthesis products, showed a higher accumulation in transgenic plants. These results provide molecular insight into how NbCIPK25 promotes the expression of genes involved in photosynthesis, thereby maintaining plant growth under salt stress. Our finding supports the potential application of halophyte-derived NbCIPK25 in genetic modification for better salt adaptation. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800929/ /pubmed/36589077 http://dx.doi.org/10.3389/fpls.2022.1052463 Text en Copyright © 2022 Lu, Wu, Tang, Zhu, Hao, Zhang, Li, Shi, Chen and Cheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Plant Science Lu, Lu Wu, Xinru Tang, Yao Zhu, Liming Hao, Zhaodong Zhang, Jingbo Li, Xinle Shi, Jisen Chen, Jinhui Cheng, Tielong Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress |
title | Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress |
title_full | Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress |
title_fullStr | Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress |
title_full_unstemmed | Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress |
title_short | Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress |
title_sort | halophyte nitraria billardieri cipk25 promotes photosynthesis in arabidopsis under salt stress |
topic | Plant Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800929/ https://www.ncbi.nlm.nih.gov/pubmed/36589077 http://dx.doi.org/10.3389/fpls.2022.1052463 |
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