Cargando…

Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress

The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation d...

Descripción completa

Detalles Bibliográficos
Autores principales: Lu, Lu, Wu, Xinru, Tang, Yao, Zhu, Liming, Hao, Zhaodong, Zhang, Jingbo, Li, Xinle, Shi, Jisen, Chen, Jinhui, Cheng, Tielong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800929/
https://www.ncbi.nlm.nih.gov/pubmed/36589077
http://dx.doi.org/10.3389/fpls.2022.1052463
_version_ 1784861392116908032
author Lu, Lu
Wu, Xinru
Tang, Yao
Zhu, Liming
Hao, Zhaodong
Zhang, Jingbo
Li, Xinle
Shi, Jisen
Chen, Jinhui
Cheng, Tielong
author_facet Lu, Lu
Wu, Xinru
Tang, Yao
Zhu, Liming
Hao, Zhaodong
Zhang, Jingbo
Li, Xinle
Shi, Jisen
Chen, Jinhui
Cheng, Tielong
author_sort Lu, Lu
collection PubMed
description The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation during abiotic stresses, which inhibits the potential application of these genes to improve environmental adaptability of glycophytes. In this study, we constructed Nitraria billardieri CIPK25 overexpressing Arabidopsis and analyzed the seedling development under salt treatment. Our results show that Arabidopsis with NbCIPK25 expression exhibits more vigorous growth than wild type plants under salt condition. To gain insight into the molecular mechanisms underlying salt tolerance, we profiled the transcriptome of WT and transgenic plants via RNA-seq. GO and KEGG analyses revealed that upregulated genes in NbCIPK25 overexpressing seedlings under salt stress are enriched in photosynthesis related terms; Calvin-cycle genes including glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) are significantly upregulated in transgenic plants, which is consistent with a decreased level of NADPH (GAPDH substrate) and increased level of NADP(+). Accordingly, NbCIPK25 overexpressing plants exhibited more efficient photosynthesis; soluble sugar and proteins, as photosynthesis products, showed a higher accumulation in transgenic plants. These results provide molecular insight into how NbCIPK25 promotes the expression of genes involved in photosynthesis, thereby maintaining plant growth under salt stress. Our finding supports the potential application of halophyte-derived NbCIPK25 in genetic modification for better salt adaptation.
format Online
Article
Text
id pubmed-9800929
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98009292022-12-31 Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress Lu, Lu Wu, Xinru Tang, Yao Zhu, Liming Hao, Zhaodong Zhang, Jingbo Li, Xinle Shi, Jisen Chen, Jinhui Cheng, Tielong Front Plant Sci Plant Science The calcineurin B-like (CBL)-interacting protein kinases (CIPKs), a type of plant-specific genes in the calcium signaling pathway, function in response to adverse environments. However, few halophyte derived CIPKs have been studied for their role in plant physiological and developmental adaptation during abiotic stresses, which inhibits the potential application of these genes to improve environmental adaptability of glycophytes. In this study, we constructed Nitraria billardieri CIPK25 overexpressing Arabidopsis and analyzed the seedling development under salt treatment. Our results show that Arabidopsis with NbCIPK25 expression exhibits more vigorous growth than wild type plants under salt condition. To gain insight into the molecular mechanisms underlying salt tolerance, we profiled the transcriptome of WT and transgenic plants via RNA-seq. GO and KEGG analyses revealed that upregulated genes in NbCIPK25 overexpressing seedlings under salt stress are enriched in photosynthesis related terms; Calvin-cycle genes including glyceraldehyde-3-phosphate dehydrogenases (GAPDHs) are significantly upregulated in transgenic plants, which is consistent with a decreased level of NADPH (GAPDH substrate) and increased level of NADP(+). Accordingly, NbCIPK25 overexpressing plants exhibited more efficient photosynthesis; soluble sugar and proteins, as photosynthesis products, showed a higher accumulation in transgenic plants. These results provide molecular insight into how NbCIPK25 promotes the expression of genes involved in photosynthesis, thereby maintaining plant growth under salt stress. Our finding supports the potential application of halophyte-derived NbCIPK25 in genetic modification for better salt adaptation. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800929/ /pubmed/36589077 http://dx.doi.org/10.3389/fpls.2022.1052463 Text en Copyright © 2022 Lu, Wu, Tang, Zhu, Hao, Zhang, Li, Shi, Chen and Cheng https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Lu, Lu
Wu, Xinru
Tang, Yao
Zhu, Liming
Hao, Zhaodong
Zhang, Jingbo
Li, Xinle
Shi, Jisen
Chen, Jinhui
Cheng, Tielong
Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
title Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
title_full Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
title_fullStr Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
title_full_unstemmed Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
title_short Halophyte Nitraria billardieri CIPK25 promotes photosynthesis in Arabidopsis under salt stress
title_sort halophyte nitraria billardieri cipk25 promotes photosynthesis in arabidopsis under salt stress
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800929/
https://www.ncbi.nlm.nih.gov/pubmed/36589077
http://dx.doi.org/10.3389/fpls.2022.1052463
work_keys_str_mv AT lulu halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT wuxinru halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT tangyao halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT zhuliming halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT haozhaodong halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT zhangjingbo halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT lixinle halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT shijisen halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT chenjinhui halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress
AT chengtielong halophytenitrariabillardiericipk25promotesphotosynthesisinarabidopsisundersaltstress