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Roles of leptin on energy balance and thermoregulation in Eothenomys miletus

Leptin is a hormone mainly synthesized and secreted by white adipose tissue (WAT), which regulates various physiological processes. To investigate the role of leptin in energy balance and thermoregulation in Eothenomys miletus, voles were randomly divided into leptin-injected and PBS-injected groups...

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Autores principales: Chen, Huibao, Zhang, Hao, Jia, Ting, Wang, Zhengkun, Zhu, Wanlong
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800980/
https://www.ncbi.nlm.nih.gov/pubmed/36589465
http://dx.doi.org/10.3389/fphys.2022.1054107
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author Chen, Huibao
Zhang, Hao
Jia, Ting
Wang, Zhengkun
Zhu, Wanlong
author_facet Chen, Huibao
Zhang, Hao
Jia, Ting
Wang, Zhengkun
Zhu, Wanlong
author_sort Chen, Huibao
collection PubMed
description Leptin is a hormone mainly synthesized and secreted by white adipose tissue (WAT), which regulates various physiological processes. To investigate the role of leptin in energy balance and thermoregulation in Eothenomys miletus, voles were randomly divided into leptin-injected and PBS-injected groups and placed at 25°C ± 1°C with a photoperiod of 12 L:12 D. They were housed under laboratory conditions for 28 days and compared in terms of body mass, food intake, water intake, core body temperature, interscapular skin temperature, resting metabolic rate (RMR), nonshivering thermogenesis (NST), liver and brown adipose tissue (BAT) thermogenic activity, and serum hormone levels. The results showed that leptin injection decreased body mass, body fat, food intake, and water intake. But it had no significant effect on carcass protein. Leptin injection increased core body temperature, interscapular skin temperature, resting metabolic rate, non-shivering thermogenesis, mitochondrial protein content and cytochrome C oxidase (COX) activity in liver and brown adipose tissue, uncoupling protein 1 (UCP1) content and thyroxin 5′-deiodinase (T(4)5′-DII) activity in brown adipose tissue significantly. Serum leptin, triiodothyronine (T(3)), thyrotropin-releasing hormone (TRH) and corticotropin-releasing hormone (CRH) concentrations were also increased significantly. Correlation analysis showed that serum leptin levels were positively correlated with core body temperature, body mass loss, uncoupling protein 1 content, thyroxin 5′-deiodinase activity, nonshivering thermogenesis, and negatively correlated with food intake; thyroxin 5′-deiodinase and triiodothyronine levels were positively correlated, suggesting that thyroxin 5′-deiodinase may play an important role in leptin-induced thermogenesis in brown adipose tissue. In conclusion, our study shows that exogenous leptin is involved in the regulation of energy metabolism and thermoregulation in E. miletus, and thyroid hormone may play an important role in the process of leptin regulating energy balance in E. miletus.
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spelling pubmed-98009802022-12-31 Roles of leptin on energy balance and thermoregulation in Eothenomys miletus Chen, Huibao Zhang, Hao Jia, Ting Wang, Zhengkun Zhu, Wanlong Front Physiol Physiology Leptin is a hormone mainly synthesized and secreted by white adipose tissue (WAT), which regulates various physiological processes. To investigate the role of leptin in energy balance and thermoregulation in Eothenomys miletus, voles were randomly divided into leptin-injected and PBS-injected groups and placed at 25°C ± 1°C with a photoperiod of 12 L:12 D. They were housed under laboratory conditions for 28 days and compared in terms of body mass, food intake, water intake, core body temperature, interscapular skin temperature, resting metabolic rate (RMR), nonshivering thermogenesis (NST), liver and brown adipose tissue (BAT) thermogenic activity, and serum hormone levels. The results showed that leptin injection decreased body mass, body fat, food intake, and water intake. But it had no significant effect on carcass protein. Leptin injection increased core body temperature, interscapular skin temperature, resting metabolic rate, non-shivering thermogenesis, mitochondrial protein content and cytochrome C oxidase (COX) activity in liver and brown adipose tissue, uncoupling protein 1 (UCP1) content and thyroxin 5′-deiodinase (T(4)5′-DII) activity in brown adipose tissue significantly. Serum leptin, triiodothyronine (T(3)), thyrotropin-releasing hormone (TRH) and corticotropin-releasing hormone (CRH) concentrations were also increased significantly. Correlation analysis showed that serum leptin levels were positively correlated with core body temperature, body mass loss, uncoupling protein 1 content, thyroxin 5′-deiodinase activity, nonshivering thermogenesis, and negatively correlated with food intake; thyroxin 5′-deiodinase and triiodothyronine levels were positively correlated, suggesting that thyroxin 5′-deiodinase may play an important role in leptin-induced thermogenesis in brown adipose tissue. In conclusion, our study shows that exogenous leptin is involved in the regulation of energy metabolism and thermoregulation in E. miletus, and thyroid hormone may play an important role in the process of leptin regulating energy balance in E. miletus. Frontiers Media S.A. 2022-12-16 /pmc/articles/PMC9800980/ /pubmed/36589465 http://dx.doi.org/10.3389/fphys.2022.1054107 Text en Copyright © 2022 Chen, Zhang, Jia, Wang and Zhu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Physiology
Chen, Huibao
Zhang, Hao
Jia, Ting
Wang, Zhengkun
Zhu, Wanlong
Roles of leptin on energy balance and thermoregulation in Eothenomys miletus
title Roles of leptin on energy balance and thermoregulation in Eothenomys miletus
title_full Roles of leptin on energy balance and thermoregulation in Eothenomys miletus
title_fullStr Roles of leptin on energy balance and thermoregulation in Eothenomys miletus
title_full_unstemmed Roles of leptin on energy balance and thermoregulation in Eothenomys miletus
title_short Roles of leptin on energy balance and thermoregulation in Eothenomys miletus
title_sort roles of leptin on energy balance and thermoregulation in eothenomys miletus
topic Physiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9800980/
https://www.ncbi.nlm.nih.gov/pubmed/36589465
http://dx.doi.org/10.3389/fphys.2022.1054107
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