Cargando…

Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia

The evolution and the development of the symptoms of Coronavirus disease 19 (COVID-19) are due to different factors, where the microbiome plays a relevant role. The possible relationships between the gut, lung, nasopharyngeal, and oral microbiome with COVID-19 have been investigated. We analyzed the...

Descripción completa

Detalles Bibliográficos
Autores principales: Nardelli, Carmela, Scaglione, Giovanni Luca, Testa, Domenico, Setaro, Mario, Russo, Filippo, Di Domenico, Carmela, Atripaldi, Lidia, Zollo, Massimo, Corrado, Federica, Salvatore, Paola, Pinchera, Biagio, Gentile, Ivan, Capoluongo, Ettore
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9802018/
https://www.ncbi.nlm.nih.gov/pubmed/36583787
http://dx.doi.org/10.1007/s00284-022-03106-x
_version_ 1784861605932040192
author Nardelli, Carmela
Scaglione, Giovanni Luca
Testa, Domenico
Setaro, Mario
Russo, Filippo
Di Domenico, Carmela
Atripaldi, Lidia
Zollo, Massimo
Corrado, Federica
Salvatore, Paola
Pinchera, Biagio
Gentile, Ivan
Capoluongo, Ettore
author_facet Nardelli, Carmela
Scaglione, Giovanni Luca
Testa, Domenico
Setaro, Mario
Russo, Filippo
Di Domenico, Carmela
Atripaldi, Lidia
Zollo, Massimo
Corrado, Federica
Salvatore, Paola
Pinchera, Biagio
Gentile, Ivan
Capoluongo, Ettore
author_sort Nardelli, Carmela
collection PubMed
description The evolution and the development of the symptoms of Coronavirus disease 19 (COVID-19) are due to different factors, where the microbiome plays a relevant role. The possible relationships between the gut, lung, nasopharyngeal, and oral microbiome with COVID-19 have been investigated. We analyzed the nasal microbiome of both positive and negative SARS-CoV-2 individuals, showing differences in terms of bacterial composition in this niche of respiratory tract. The microbiota solution A (Arrow Diagnostics) was used to cover the hypervariable V1–V3 regions of the bacterial 16S rRNA gene. MicrobAT Suite and MicrobiomeAnalyst program were used to identify the operational taxonomic units (OTUs) and to perform the statistical analysis, respectively. The main taxa identified in nasal microbiome of COVID-19 patients and in Healthy Control subjects belonged to three distinct phyla: Proteobacteria (HC = 14%, Cov19 = 35.8%), Firmicutes (HC = 28.8%, Cov19 = 30.6%), and Actinobacteria (HC = 56.7%, Cov19 = 14.4%) with a relative abundance > 1% in all groups. A significant reduction of Actinobacteria in Cov19 group compared to controls (P < 0.001, FDR = 0.01) was found. The significant reduction of Actinobacteria was identified in all taxonomic levels down to the genus (P < 0.01) using the ANOVA test. Indeed, a significantly reduced relative abundance of Corynebacterium was found in the patients compared to healthy controls (P = 0.001). Reduced abundance of Corynebacterium has been widely associated with anosmia, a common symptom of COVID-19 as suffered from our patients. Contrastingly, the Corynebacterium genus was highly represented in the nasal mucosa of healthy subjects. Further investigations on larger cohorts are necessary to establish functional relationships between nasal microbiota content and clinical features of COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-022-03106-x.
format Online
Article
Text
id pubmed-9802018
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Springer US
record_format MEDLINE/PubMed
spelling pubmed-98020182023-01-01 Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia Nardelli, Carmela Scaglione, Giovanni Luca Testa, Domenico Setaro, Mario Russo, Filippo Di Domenico, Carmela Atripaldi, Lidia Zollo, Massimo Corrado, Federica Salvatore, Paola Pinchera, Biagio Gentile, Ivan Capoluongo, Ettore Curr Microbiol Short Communication The evolution and the development of the symptoms of Coronavirus disease 19 (COVID-19) are due to different factors, where the microbiome plays a relevant role. The possible relationships between the gut, lung, nasopharyngeal, and oral microbiome with COVID-19 have been investigated. We analyzed the nasal microbiome of both positive and negative SARS-CoV-2 individuals, showing differences in terms of bacterial composition in this niche of respiratory tract. The microbiota solution A (Arrow Diagnostics) was used to cover the hypervariable V1–V3 regions of the bacterial 16S rRNA gene. MicrobAT Suite and MicrobiomeAnalyst program were used to identify the operational taxonomic units (OTUs) and to perform the statistical analysis, respectively. The main taxa identified in nasal microbiome of COVID-19 patients and in Healthy Control subjects belonged to three distinct phyla: Proteobacteria (HC = 14%, Cov19 = 35.8%), Firmicutes (HC = 28.8%, Cov19 = 30.6%), and Actinobacteria (HC = 56.7%, Cov19 = 14.4%) with a relative abundance > 1% in all groups. A significant reduction of Actinobacteria in Cov19 group compared to controls (P < 0.001, FDR = 0.01) was found. The significant reduction of Actinobacteria was identified in all taxonomic levels down to the genus (P < 0.01) using the ANOVA test. Indeed, a significantly reduced relative abundance of Corynebacterium was found in the patients compared to healthy controls (P = 0.001). Reduced abundance of Corynebacterium has been widely associated with anosmia, a common symptom of COVID-19 as suffered from our patients. Contrastingly, the Corynebacterium genus was highly represented in the nasal mucosa of healthy subjects. Further investigations on larger cohorts are necessary to establish functional relationships between nasal microbiota content and clinical features of COVID-19. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s00284-022-03106-x. Springer US 2022-12-30 2023 /pmc/articles/PMC9802018/ /pubmed/36583787 http://dx.doi.org/10.1007/s00284-022-03106-x Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Short Communication
Nardelli, Carmela
Scaglione, Giovanni Luca
Testa, Domenico
Setaro, Mario
Russo, Filippo
Di Domenico, Carmela
Atripaldi, Lidia
Zollo, Massimo
Corrado, Federica
Salvatore, Paola
Pinchera, Biagio
Gentile, Ivan
Capoluongo, Ettore
Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia
title Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia
title_full Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia
title_fullStr Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia
title_full_unstemmed Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia
title_short Nasal Microbiome in COVID-19: A Potential Role of Corynebacterium in Anosmia
title_sort nasal microbiome in covid-19: a potential role of corynebacterium in anosmia
topic Short Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9802018/
https://www.ncbi.nlm.nih.gov/pubmed/36583787
http://dx.doi.org/10.1007/s00284-022-03106-x
work_keys_str_mv AT nardellicarmela nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT scaglionegiovanniluca nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT testadomenico nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT setaromario nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT russofilippo nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT didomenicocarmela nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT atripaldilidia nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT zollomassimo nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT corradofederica nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT salvatorepaola nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT pincherabiagio nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT gentileivan nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia
AT capoluongoettore nasalmicrobiomeincovid19apotentialroleofcorynebacteriuminanosmia