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Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds

The treatment of diabetic wound is a focus issue. At present, the Nocardia rubra cell wall skeleton (Nr-CWS) has been proved proven to promote angiogenesis and wound repair. Unfortunately, the high-glucose diabetic wound environment makes many drugs unable to be released effectively, and soon be rem...

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Detalles Bibliográficos
Autores principales: Wang, Jian, Zhao, Bingkun, Sun, Lili, Jiang, Liqun, Li, Qiang, Jin, Peisheng
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803202/
https://www.ncbi.nlm.nih.gov/pubmed/36584197
http://dx.doi.org/10.1371/journal.pone.0279727
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author Wang, Jian
Zhao, Bingkun
Sun, Lili
Jiang, Liqun
Li, Qiang
Jin, Peisheng
author_facet Wang, Jian
Zhao, Bingkun
Sun, Lili
Jiang, Liqun
Li, Qiang
Jin, Peisheng
author_sort Wang, Jian
collection PubMed
description The treatment of diabetic wound is a focus issue. At present, the Nocardia rubra cell wall skeleton (Nr-CWS) has been proved proven to promote angiogenesis and wound repair. Unfortunately, the high-glucose diabetic wound environment makes many drugs unable to be released effectively, and soon be removed. Smart thermosensitive poloxamer hydrogel (TH) is an ideal and adjustable drug delivery platform compatible with most living tissues. Here, a multifunctional composite thermosensitive hydrogel was developed. A mixture of poloxamers 407 and 188 as the gel matrix, and then it was physically mixed with Nr-CWS. The delivery vehicle not only controlled its release stably, preventing degradation in vitro, but also showed good affinity in vitro. In vivo, compared with thermosensitive poloxamer hydrogel alone or the direct use of Nr-CWS, the thermosensitive poloxamer hydrogel loaded with Nr-CWS promoted the proliferation of vascular endothelial cells effectively, resulting in increased expression of derma-related structural proteins and enhanced angiogenesis and wound healing. This study indicated that the angiogenesis and skin regeneration brought by Nr-CWS hydrogel are related to the activation of phosphatidylinositol 3 kinase and protein kinase B, Janus kinase/signal transducer and activator of transcription, and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase signaling pathways.
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spelling pubmed-98032022022-12-31 Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds Wang, Jian Zhao, Bingkun Sun, Lili Jiang, Liqun Li, Qiang Jin, Peisheng PLoS One Research Article The treatment of diabetic wound is a focus issue. At present, the Nocardia rubra cell wall skeleton (Nr-CWS) has been proved proven to promote angiogenesis and wound repair. Unfortunately, the high-glucose diabetic wound environment makes many drugs unable to be released effectively, and soon be removed. Smart thermosensitive poloxamer hydrogel (TH) is an ideal and adjustable drug delivery platform compatible with most living tissues. Here, a multifunctional composite thermosensitive hydrogel was developed. A mixture of poloxamers 407 and 188 as the gel matrix, and then it was physically mixed with Nr-CWS. The delivery vehicle not only controlled its release stably, preventing degradation in vitro, but also showed good affinity in vitro. In vivo, compared with thermosensitive poloxamer hydrogel alone or the direct use of Nr-CWS, the thermosensitive poloxamer hydrogel loaded with Nr-CWS promoted the proliferation of vascular endothelial cells effectively, resulting in increased expression of derma-related structural proteins and enhanced angiogenesis and wound healing. This study indicated that the angiogenesis and skin regeneration brought by Nr-CWS hydrogel are related to the activation of phosphatidylinositol 3 kinase and protein kinase B, Janus kinase/signal transducer and activator of transcription, and mitogen-activated protein kinase kinase/extracellular signal-regulated kinase signaling pathways. Public Library of Science 2022-12-30 /pmc/articles/PMC9803202/ /pubmed/36584197 http://dx.doi.org/10.1371/journal.pone.0279727 Text en © 2022 Wang et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Wang, Jian
Zhao, Bingkun
Sun, Lili
Jiang, Liqun
Li, Qiang
Jin, Peisheng
Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds
title Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds
title_full Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds
title_fullStr Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds
title_full_unstemmed Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds
title_short Smart thermosensitive poloxamer hydrogels loaded with Nr-CWs for the treatment of diabetic wounds
title_sort smart thermosensitive poloxamer hydrogels loaded with nr-cws for the treatment of diabetic wounds
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803202/
https://www.ncbi.nlm.nih.gov/pubmed/36584197
http://dx.doi.org/10.1371/journal.pone.0279727
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