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Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism
Xanthii fructus (XF) is the dried and mature fruit of Xanthium sibiricum Patr. It has the effects of anti-inflammatory, antioxidant and anti-arthritic. Rheumatoid arthritis (RA) is the most common inflammatory disorder and often leads to disability. However, there are few studies on the treatment of...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Lippincott Williams & Wilkins
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803504/ https://www.ncbi.nlm.nih.gov/pubmed/36596012 http://dx.doi.org/10.1097/MD.0000000000032541 |
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author | Jiang, Hai Yu, Huan Zheng, Senwang Wang, Xuejiao Hou, Ajiao Kuang, Haixue Yang, Liu |
author_facet | Jiang, Hai Yu, Huan Zheng, Senwang Wang, Xuejiao Hou, Ajiao Kuang, Haixue Yang, Liu |
author_sort | Jiang, Hai |
collection | PubMed |
description | Xanthii fructus (XF) is the dried and mature fruit of Xanthium sibiricum Patr. It has the effects of anti-inflammatory, antioxidant and anti-arthritic. Rheumatoid arthritis (RA) is the most common inflammatory disorder and often leads to disability. However, there are few studies on the treatment of RA by XF and the specific mechanism of treatment has not been clarified. This study was designed to explore the effects of proliferation and apoptosis by XF on human fibroblast-like synovial-RA (HFLS-RA) cells and investigate its mechanism. The cell proliferation ability was detected by MTS assay. Hoechst 33,342 staining was used to detect apoptosis, and the apoptosis rate was detected by flow cytometry. The expression levels of NF-κB p65 and β-catenin were detected by Western Blotting. MTS, Hoechst 33,342, flow cytometry analysis showed that the alcohol extract of XF inhibited human fibroblast-like synovial-RA cells proliferation and promoted apoptosis in a dose-dependent manner. Western Blotting experiment showed that the extract of XF could reduce the expression levels of NF-κB p65 and β-catenin. The extract of XF has a significant therapeutic effect on RA in vitro by regulating NF-κB signaling pathway and Wnt/β-catenin signaling pathway. Our research will help to clarify the potential pharmacological mechanism of XF on RA and provide experimental basis for the application of XF in clinical treatment. |
format | Online Article Text |
id | pubmed-9803504 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Lippincott Williams & Wilkins |
record_format | MEDLINE/PubMed |
spelling | pubmed-98035042023-01-03 Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism Jiang, Hai Yu, Huan Zheng, Senwang Wang, Xuejiao Hou, Ajiao Kuang, Haixue Yang, Liu Medicine (Baltimore) 6900 Xanthii fructus (XF) is the dried and mature fruit of Xanthium sibiricum Patr. It has the effects of anti-inflammatory, antioxidant and anti-arthritic. Rheumatoid arthritis (RA) is the most common inflammatory disorder and often leads to disability. However, there are few studies on the treatment of RA by XF and the specific mechanism of treatment has not been clarified. This study was designed to explore the effects of proliferation and apoptosis by XF on human fibroblast-like synovial-RA (HFLS-RA) cells and investigate its mechanism. The cell proliferation ability was detected by MTS assay. Hoechst 33,342 staining was used to detect apoptosis, and the apoptosis rate was detected by flow cytometry. The expression levels of NF-κB p65 and β-catenin were detected by Western Blotting. MTS, Hoechst 33,342, flow cytometry analysis showed that the alcohol extract of XF inhibited human fibroblast-like synovial-RA cells proliferation and promoted apoptosis in a dose-dependent manner. Western Blotting experiment showed that the extract of XF could reduce the expression levels of NF-κB p65 and β-catenin. The extract of XF has a significant therapeutic effect on RA in vitro by regulating NF-κB signaling pathway and Wnt/β-catenin signaling pathway. Our research will help to clarify the potential pharmacological mechanism of XF on RA and provide experimental basis for the application of XF in clinical treatment. Lippincott Williams & Wilkins 2022-12-30 /pmc/articles/PMC9803504/ /pubmed/36596012 http://dx.doi.org/10.1097/MD.0000000000032541 Text en Copyright © 2022 the Author(s). Published by Wolters Kluwer Health, Inc. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License 4.0 (CCBY) (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | 6900 Jiang, Hai Yu, Huan Zheng, Senwang Wang, Xuejiao Hou, Ajiao Kuang, Haixue Yang, Liu Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism |
title | Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism |
title_full | Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism |
title_fullStr | Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism |
title_full_unstemmed | Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism |
title_short | Effect of Xanthii Fructus alcohol extract on proliferation and apoptosis of HFLS-RA and its mechanism |
title_sort | effect of xanthii fructus alcohol extract on proliferation and apoptosis of hfls-ra and its mechanism |
topic | 6900 |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803504/ https://www.ncbi.nlm.nih.gov/pubmed/36596012 http://dx.doi.org/10.1097/MD.0000000000032541 |
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