An optimized disulfide cross-linking protocol to determine interactions of proteins produced in Escherichia coli

Protein-protein interactions play important roles in regulating cellular functions. We present an optimized disulfide cross-linking protocol for testing predicted interactions of soluble or membrane proteins. Coexpression in E. coli of proteins with a single cysteine residue results in disulfide bon...

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Detalles Bibliográficos
Autores principales: Olenic, Sandra, Kroos, Lee
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803820/
https://www.ncbi.nlm.nih.gov/pubmed/36566383
http://dx.doi.org/10.1016/j.xpro.2022.101962

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803820/
https://www.ncbi.nlm.nih.gov/pubmed/36566383
http://dx.doi.org/10.1016/j.xpro.2022.101962

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