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An optimized disulfide cross-linking protocol to determine interactions of proteins produced in Escherichia coli
Protein-protein interactions play important roles in regulating cellular functions. We present an optimized disulfide cross-linking protocol for testing predicted interactions of soluble or membrane proteins. Coexpression in E. coli of proteins with a single cysteine residue results in disulfide bon...
| Autores principales: | , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803820/ https://www.ncbi.nlm.nih.gov/pubmed/36566383 http://dx.doi.org/10.1016/j.xpro.2022.101962 |