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Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter

To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield of cell number and viability. Knowledge of cell volumet...

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Autores principales: Drobek, Christoph, Meyer, Juliane, Mau, Robert, Wolff, Anne, Peters, Kirsten, Seitz, Hermann
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803822/
https://www.ncbi.nlm.nih.gov/pubmed/36594013
http://dx.doi.org/10.1016/j.isci.2022.105796
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author Drobek, Christoph
Meyer, Juliane
Mau, Robert
Wolff, Anne
Peters, Kirsten
Seitz, Hermann
author_facet Drobek, Christoph
Meyer, Juliane
Mau, Robert
Wolff, Anne
Peters, Kirsten
Seitz, Hermann
author_sort Drobek, Christoph
collection PubMed
description To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield of cell number and viability. Knowledge of cell volumetric mass density is important for experimental and numerical optimization of these procedures. Although methods for measuring cell volumetric mass density already exist, they either consume much time and cell material or require a special setup. Therefore, we developed a user-friendly method that is based on the use of readily available instrumentation. The newly developed method is predicated on the linear relationship between the volumetric mass density of the cell suspension and the volumetric mass density, number, and diameter of the cells in the suspension. We used this method to determine the volumetric mass density of mesenchymal stem cells (MSCs) and compared it to results from the established density centrifugation.
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spelling pubmed-98038222023-01-01 Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter Drobek, Christoph Meyer, Juliane Mau, Robert Wolff, Anne Peters, Kirsten Seitz, Hermann iScience Article To use regeneratively active cells for cell therapeutic applications, the cells must be isolated from their resident tissues. Different isolation procedures subject these cells to varying degrees of mechanical strain, which can affect the yield of cell number and viability. Knowledge of cell volumetric mass density is important for experimental and numerical optimization of these procedures. Although methods for measuring cell volumetric mass density already exist, they either consume much time and cell material or require a special setup. Therefore, we developed a user-friendly method that is based on the use of readily available instrumentation. The newly developed method is predicated on the linear relationship between the volumetric mass density of the cell suspension and the volumetric mass density, number, and diameter of the cells in the suspension. We used this method to determine the volumetric mass density of mesenchymal stem cells (MSCs) and compared it to results from the established density centrifugation. Elsevier 2022-12-10 /pmc/articles/PMC9803822/ /pubmed/36594013 http://dx.doi.org/10.1016/j.isci.2022.105796 Text en © 2022 The Author(s) https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Drobek, Christoph
Meyer, Juliane
Mau, Robert
Wolff, Anne
Peters, Kirsten
Seitz, Hermann
Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
title Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
title_full Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
title_fullStr Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
title_full_unstemmed Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
title_short Volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
title_sort volumetric mass density measurements of mesenchymal stem cells in suspension using a density meter
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803822/
https://www.ncbi.nlm.nih.gov/pubmed/36594013
http://dx.doi.org/10.1016/j.isci.2022.105796
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