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Dataset from de novo transcriptome assembly of Myristica fatua leaves using MinION nanopore sequencer

Myristica fatua is a tropical fruit tree species originating from Indonesia. Very few genomic resources are available for the species. We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). The assemb...

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Detalles Bibliográficos
Autores principales: Matra, Deden Derajat, Adrian, M, Karmanah, Kusuma, Jakty, Duminil, Jérôme, Sobir, Poerwanto, Roedhy
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803912/
https://www.ncbi.nlm.nih.gov/pubmed/36593766
http://dx.doi.org/10.1016/j.dib.2022.108838
Descripción
Sumario:Myristica fatua is a tropical fruit tree species originating from Indonesia. Very few genomic resources are available for the species. We developed a full-length transcriptome assembly using long-read sequencing (MinION Nanopore technology) and produced 4.3 million reads (3.5 G of bases). The assembled full-length transcript was constructed using the RATTLE program and assembled 21,098 transcripts. The transcript ranged from 201 – 14,174 bp, and N50 was 2,017 bp. The transcripts were annotated with the UNIPROT database using BlastX. The functional annotation was performed using Blast2go software. The 8,445 microsatellite motif-containing contigs were identified. The raw reads are deposited in the ENA (European Nucleotide Archive) with ENA experiment accession number ERX6798613.