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Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7

INTRODUCTION: To investigate the protective effect of sevoflurane preconditioning on renal ischemia‐reperfusion injury (renalischemiareperfusionmodel, RIRI) and its related mechanism. METHODS: Eighty healthy adult male SD rats were randomly divided into control group (Sham group), model group (RIRI...

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Autores principales: Xu, Xudong, Deng, Rongrong, Zou, Lu, Pan, Xiaoyan, Sheng, Zhifeng, Xu, Da, Gan, Tingting
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803933/
https://www.ncbi.nlm.nih.gov/pubmed/36705408
http://dx.doi.org/10.1002/iid3.753
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author Xu, Xudong
Deng, Rongrong
Zou, Lu
Pan, Xiaoyan
Sheng, Zhifeng
Xu, Da
Gan, Tingting
author_facet Xu, Xudong
Deng, Rongrong
Zou, Lu
Pan, Xiaoyan
Sheng, Zhifeng
Xu, Da
Gan, Tingting
author_sort Xu, Xudong
collection PubMed
description INTRODUCTION: To investigate the protective effect of sevoflurane preconditioning on renal ischemia‐reperfusion injury (renalischemiareperfusionmodel, RIRI) and its related mechanism. METHODS: Eighty healthy adult male SD rats were randomly divided into control group (Sham group), model group (RIRI group), sevoflurane pretreatment group (Sev group) and TRPM7 inhibitor combined with sevoflurane pretreatment group (T + Sev group), 20 animals in each group. Hematoxylin‐eosin (HE) staining was used to observe the pathological changes of renal tissue, and the levels of creatinine and urea nitrogen in each group were detected. Deoxyribonucleic acid terminal transferase‐mediated dUTP nick end labeling (TUNEL) assay was used to detect renal cell apoptosis, and Western blottingwas used to detect the expression of apoptotic proteins cleaved‐caspase‐3, bax, Bcl‐2, and TRPM7 in renal tissue; Detection of oxidative stress‐related index levels in renal tissue and levels of inflammatory factors in renal tissue and serum. RESULTS: Compared with the Sham group, the renal tissue pathological damage was aggravated, the levels of creatinine and blood urea nitrogen were increased, and the apoptosis was increased in the RIR group and the Sev group. Death, malondialdehyde (MDA) levels and inflammatory factors were increased, and superoxide dismutase (SOD) levels were decreased (all p < .05); The scores, apoptosis rate, MDA level, and relative expression of inflammatory factor levels were decreased, and SOD levels were increased (all p < .05). Compared with the Sev group, the renal tissue pathological damage in the T + Sev group was aggravated, creatinine, blood urea nitrogen levels increased, apoptosis increased, apoptosis‐related proteins cleaved‐caspase‐3, bax, Bcl‐2 showed increased apoptosis, malondialdehyde (MDA) levels, inflammatory factor levels increased, ultrahigh The levels of oxide dismutase (SOD) were decreased (all p < .05). CONCLUSIONS: Therefore, we believe that sevoflurane is involved in the protection of rat renal ischemia‐reperfusion injury by downregulating the expression of TRPM7.
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spelling pubmed-98039332023-01-04 Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7 Xu, Xudong Deng, Rongrong Zou, Lu Pan, Xiaoyan Sheng, Zhifeng Xu, Da Gan, Tingting Immun Inflamm Dis Original Articles INTRODUCTION: To investigate the protective effect of sevoflurane preconditioning on renal ischemia‐reperfusion injury (renalischemiareperfusionmodel, RIRI) and its related mechanism. METHODS: Eighty healthy adult male SD rats were randomly divided into control group (Sham group), model group (RIRI group), sevoflurane pretreatment group (Sev group) and TRPM7 inhibitor combined with sevoflurane pretreatment group (T + Sev group), 20 animals in each group. Hematoxylin‐eosin (HE) staining was used to observe the pathological changes of renal tissue, and the levels of creatinine and urea nitrogen in each group were detected. Deoxyribonucleic acid terminal transferase‐mediated dUTP nick end labeling (TUNEL) assay was used to detect renal cell apoptosis, and Western blottingwas used to detect the expression of apoptotic proteins cleaved‐caspase‐3, bax, Bcl‐2, and TRPM7 in renal tissue; Detection of oxidative stress‐related index levels in renal tissue and levels of inflammatory factors in renal tissue and serum. RESULTS: Compared with the Sham group, the renal tissue pathological damage was aggravated, the levels of creatinine and blood urea nitrogen were increased, and the apoptosis was increased in the RIR group and the Sev group. Death, malondialdehyde (MDA) levels and inflammatory factors were increased, and superoxide dismutase (SOD) levels were decreased (all p < .05); The scores, apoptosis rate, MDA level, and relative expression of inflammatory factor levels were decreased, and SOD levels were increased (all p < .05). Compared with the Sev group, the renal tissue pathological damage in the T + Sev group was aggravated, creatinine, blood urea nitrogen levels increased, apoptosis increased, apoptosis‐related proteins cleaved‐caspase‐3, bax, Bcl‐2 showed increased apoptosis, malondialdehyde (MDA) levels, inflammatory factor levels increased, ultrahigh The levels of oxide dismutase (SOD) were decreased (all p < .05). CONCLUSIONS: Therefore, we believe that sevoflurane is involved in the protection of rat renal ischemia‐reperfusion injury by downregulating the expression of TRPM7. John Wiley and Sons Inc. 2022-12-31 /pmc/articles/PMC9803933/ /pubmed/36705408 http://dx.doi.org/10.1002/iid3.753 Text en © 2022 The Authors. Immunity, Inflammation and Disease published by John Wiley & Sons Ltd. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Articles
Xu, Xudong
Deng, Rongrong
Zou, Lu
Pan, Xiaoyan
Sheng, Zhifeng
Xu, Da
Gan, Tingting
Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7
title Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7
title_full Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7
title_fullStr Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7
title_full_unstemmed Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7
title_short Sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of TRPM7
title_sort sevoflurane participates in the protection of rat renal ischemia‐reperfusion injury by down‐regulating the expression of trpm7
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9803933/
https://www.ncbi.nlm.nih.gov/pubmed/36705408
http://dx.doi.org/10.1002/iid3.753
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