A novel base editor SpRY-ABE8e(F148A) mediates efficient A-to-G base editing with a reduced off-target effect

Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant. By combining the protospacer adjacent motif (PAM)less SpRY nuclease with F148A-mutated TadA∗8e deaminas...

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Detalles Bibliográficos
Autores principales: Li, Guo, Cheng, Yaxian, Li, Yeqiu, Ma, Hongru, Pu, Zhongji, Li, Sa, Zhao, Yiqiang, Huang, Xingxu, Yao, Yuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2022
Materias:
Original Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804010/
https://www.ncbi.nlm.nih.gov/pubmed/36618266
http://dx.doi.org/10.1016/j.omtn.2022.12.001

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804010/
https://www.ncbi.nlm.nih.gov/pubmed/36618266
http://dx.doi.org/10.1016/j.omtn.2022.12.001

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