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A novel base editor SpRY-ABE8e(F148A) mediates efficient A-to-G base editing with a reduced off-target effect
Adenine base editors (ABEs) can mediate two transition mutations, A-to-G and T-to-C, which are suitable for repairing G·C-to-T·A pathogenic variants, the most significant human pathogenic variant. By combining the protospacer adjacent motif (PAM)less SpRY nuclease with F148A-mutated TadA∗8e deaminas...
| Autores principales: | , , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
American Society of Gene & Cell Therapy
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804010/ https://www.ncbi.nlm.nih.gov/pubmed/36618266 http://dx.doi.org/10.1016/j.omtn.2022.12.001 |