Cargando…

Total Chemical Synthesis of a Functionalized GFP Nanobody

Chemical protein synthesis has proven to be a powerful tool to access homogenously modified proteins. The chemical synthesis of nanobodies (Nb) would create possibilities to design tailored Nbs with a range of chemical modifications such as tags, linkers, reporter groups, and subsequently, Nb‐drug c...

Descripción completa

Detalles Bibliográficos
Autores principales:	Huppelschoten, Yara, Elhebieshy, Angela F., Hameed, Dharjath S., Sapmaz, Aysegul, Buchardt, Jens, Nielsen, Thomas E., Ovaa, Huib, van der Heden van Noort, Gerbrand J.
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	John Wiley and Sons Inc. 2022
Materias:	Research Articles
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804225/ https://www.ncbi.nlm.nih.gov/pubmed/35920208 http://dx.doi.org/10.1002/cbic.202200304

Ejemplares similares

Total Chemical Synthesis of LC3A and LC3B Activity-Based Probes
por: Huppelschoten, Yara, et al.
Publicado: (2023)

Inhibiting UCH-L5: Rational Design of a Cyclic Ubiquitin-Based Peptide Inhibitor
por: Hameed, Dharjath S., et al.
Publicado: (2022)

In‐Plate Chemical Synthesis of Isopeptide‐Linked SUMOylated Peptide Fluorescence Polarization Reagents for High‐Throughput Screening of SENP Preferences
por: Huppelschoten, Yara, et al.
Publicado: (2022)

How to Target Viral and Bacterial Effector Proteins Interfering with Ubiquitin Signaling
por: van der Heden van Noort, Gerbrand J., et al.
Publicado: (2018)

Synthesis of Poly-Ubiquitin Chains Using a Bifunctional Ubiquitin Monomer
por: van der Heden van Noort, Gerbrand J., et al.
Publicado: (2017)

Synthetic and semi-synthetic strategies to study ubiquitin signaling
por: van Tilburg, Gabriëlle BA, et al.
Publicado: (2016)

A General Chemical Ligation Approach Towards Isopeptide-Linked Ubiquitin and Ubiquitin-Like Assay Reagents
por: Geurink, Paul P, et al.
Publicado: (2012)

Chemical Tools to Study Protein ADP-Ribosylation
por: van der Heden van Noort, Gerbrand J.
Publicado: (2020)

Recognition of Lys48-Linked Di-ubiquitin and Deubiquitinating Activities of the SARS Coronavirus Papain-like Protease
por: Békés, Miklós, et al.
Publicado: (2016)

Chemical Toolkit for PARK7: Potent, Selective, and High-Throughput
por: Jia, Yuqing, et al.
Publicado: (2022)

Highlighting the Proteasome: Using Fluorescence to Visualize Proteasome Activity and Distribution
por: Gan, Jin, et al.
Publicado: (2019)

Profiling DUBs and Ubl-specific proteases with activity-based probes
por: Geurink, Paul P., et al.
Publicado: (2019)

Total Chemical Synthesis of SUMO and SUMO‐Based Probes for Profiling the Activity of SUMO‐Specific Proteases
por: Mulder, Monique P. C., et al.
Publicado: (2018)

Non-hydrolyzable Diubiquitin Probes Reveal Linkage-Specific Reactivity of Deubiquitylating Enzymes Mediated by S2 Pockets
por: Flierman, Dennis, et al.
Publicado: (2016)

Generation of the UFM1 Toolkit for Profiling UFM1‐Specific Proteases and Ligases
por: Witting, Katharina F., et al.
Publicado: (2018)

Extreme thermal stability of the antiGFP nanobody – GFP complex
por: Kakasi, Balázs, et al.
Publicado: (2023)

Chemical Synthesis of Ubiquitin, Ubiquitin-Based Probes, and Diubiquitin**
por: El Oualid, Farid, et al.
Publicado: (2010)

Bacterial OTU deubiquitinases regulate substrate ubiquitination upon Legionella infection
por: Shin, Donghyuk, et al.
Publicado: (2020)

Development of Tyrphostin Analogues to Study Inhibition of the Mycobacterium tuberculosis Pup Proteasome System
por: Janssen, Guido V., et al.
Publicado: (2021)

Development of ADPribosyl Ubiquitin Analogues to Study Enzymes Involved in Legionella Infection
por: Kim, Robbert Q., et al.
Publicado: (2020)

Neutron-encoded diubiquitins to profile linkage selectivity of deubiquitinating enzymes
por: van Tol, Bianca D. M., et al.
Publicado: (2023)

Nedd8 hydrolysis by UCH proteases in Plasmodium parasites
por: Karpiyevich, Maryia, et al.
Publicado: (2019)

Synthesis of Stable NAD(+) Mimics as Inhibitors for the Legionella pneumophila Phosphoribosyl Ubiquitylating Enzyme SdeC
por: Madern, Jerre M., et al.
Publicado: (2020)

Development of a DUB-selective fluorogenic substrate
por: Gjonaj, Lorina, et al.
Publicado: (2019)

GFP nanobodies reveal recently-exocytosed pHluorin molecules
por: Seitz, Katharina J., et al.
Publicado: (2019)

Diubiquitin-Based NMR Analysis: Interactions Between Lys6-Linked diUb and UBA Domain of UBXN1
por: Shahul Hameed, Dharjath, et al.
Publicado: (2020)

Unintended perturbation of protein function using GFP nanobodies in human cells
por: Küey, Cansu, et al.
Publicado: (2019)

Influence of nanobody binding on fluorescence emission, mobility, and organization of GFP-tagged proteins
por: Schneider, Falk, et al.
Publicado: (2020)

Linkage-specific ubiquitin chain formation depends on a lysine hydrocarbon ruler
por: Liwocha, Joanna, et al.
Publicado: (2020)

Chemical Evolution of Autotaxin Inhibitors
por: Albers, Harald M. H. G., et al.
Publicado: (2012)

Small-Molecule Activity-Based Probe for Monitoring Ubiquitin C-Terminal Hydrolase L1 (UCHL1) Activity in Live Cells and Zebrafish Embryos
por: Kooij, Raymond, et al.
Publicado: (2020)

Structure-based engineering of anti-GFP nanobody tandems as ultra-high-affinity reagents for purification
por: Zhang, Ziyue, et al.
Publicado: (2020)

Cellular Validation of a Chemically Improved Inhibitor Identifies Monoubiquitination on OTUB2
por: Gan, Jin, et al.
Publicado: (2023)

Arginine ADP-Ribosylation: Chemical Synthesis of Post-Translationally Modified Ubiquitin Proteins
por: Voorneveld, Jim, et al.
Publicado: (2022)

A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity
por: Wendel, Sofie, et al.
Publicado: (2016)

Structural insights into ubiquitin chain cleavage by Legionella ovarian tumor deubiquitinases
por: Kang, Sangwoo, et al.
Publicado: (2023)

Tripartite split-GFP assay to identify selective intracellular nanobody that suppresses GTPase RHOA subfamily downstream signaling
por: Keller, Laura, et al.
Publicado: (2022)

Chemical Tools and Biochemical Assays for SUMO Specific Proteases (SENPs)
por: Jia, Yuqing, et al.
Publicado: (2019)

Play the plug: How bacteria modify recognition by host receptors?
por: Tiruvayipati, Suma, et al.
Publicado: (2022)

Papain-like protease regulates SARS-CoV-2 viral spread and innate immunity
por: Shin, Donghyuk, et al.
Publicado: (2020)

Cannot write session to /tmp/vufind_sessions/sess_o5a1obclepu9d7pqhjb531qqcj