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Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis

The high potential of exhaled breath for disease diagnosis has been highlighted in numerous studies. However, exhaled breath analysis is suffering from a lack of standardized sampling and analysis procedures, impacting the robustness of inter‐laboratory results, and thus hampering proper external va...

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Autores principales: Zanella, Delphine, Henin, Adèle, Mascrez, Steven, Stefanuto, Pierre‐Hugues, Franchina, Flavio Antonio, Focant, Jean‐François, Purcaro, Giorgia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804543/
https://www.ncbi.nlm.nih.gov/pubmed/35853166
http://dx.doi.org/10.1002/jssc.202200164
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author Zanella, Delphine
Henin, Adèle
Mascrez, Steven
Stefanuto, Pierre‐Hugues
Franchina, Flavio Antonio
Focant, Jean‐François
Purcaro, Giorgia
author_facet Zanella, Delphine
Henin, Adèle
Mascrez, Steven
Stefanuto, Pierre‐Hugues
Franchina, Flavio Antonio
Focant, Jean‐François
Purcaro, Giorgia
author_sort Zanella, Delphine
collection PubMed
description The high potential of exhaled breath for disease diagnosis has been highlighted in numerous studies. However, exhaled breath analysis is suffering from a lack of standardized sampling and analysis procedures, impacting the robustness of inter‐laboratory results, and thus hampering proper external validation. The aim of this work was to verify compliance and validate the performance of two different comprehensive two‐dimensional gas chromatography coupled to mass spectrometry platforms in different laboratories by monitoring probe metabolites in exhaled breath following the Peppermint Initiative guidelines. An initial assessment of the exhaled breath sampling conditions was performed, selecting the most suitable sampling bag material and volume. Then, a single sampling was performed using Tedlar bags, followed by the trapping of the volatile organic compounds into thermal desorption tubes for the subsequent analysis using two different analytical platforms. The thermal desorption tubes were first analyzed by a (cryogenically modulated) comprehensive two‐dimensional gas chromatography system coupled to high‐resolution time‐of‐flight mass spectrometry. The desorption was performed in split mode and the split part was recollected in the same tube and further analyzed by a different (flow modulated) comprehensive two‐dimensional gas chromatography system with a parallel detection, specifically using a quadrupole mass spectrometer and a vacuum ultraviolet detector. Both the comprehensive two‐dimensional gas chromatography platforms enabled the longitudinal tracking of the peppermint oil metabolites in exhaled breath. The increased sensitivity of comprehensive two‐dimensional gas chromatography enabled to successfully monitor over a 6.5 h period a total of 10 target compounds, namely α‐pinene, camphene, β‐pinene, limonene, cymene, eucalyptol, menthofuran, menthone, isomenthone, and neomenthol.
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spelling pubmed-98045432023-01-03 Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis Zanella, Delphine Henin, Adèle Mascrez, Steven Stefanuto, Pierre‐Hugues Franchina, Flavio Antonio Focant, Jean‐François Purcaro, Giorgia J Sep Sci Gas Chromatography The high potential of exhaled breath for disease diagnosis has been highlighted in numerous studies. However, exhaled breath analysis is suffering from a lack of standardized sampling and analysis procedures, impacting the robustness of inter‐laboratory results, and thus hampering proper external validation. The aim of this work was to verify compliance and validate the performance of two different comprehensive two‐dimensional gas chromatography coupled to mass spectrometry platforms in different laboratories by monitoring probe metabolites in exhaled breath following the Peppermint Initiative guidelines. An initial assessment of the exhaled breath sampling conditions was performed, selecting the most suitable sampling bag material and volume. Then, a single sampling was performed using Tedlar bags, followed by the trapping of the volatile organic compounds into thermal desorption tubes for the subsequent analysis using two different analytical platforms. The thermal desorption tubes were first analyzed by a (cryogenically modulated) comprehensive two‐dimensional gas chromatography system coupled to high‐resolution time‐of‐flight mass spectrometry. The desorption was performed in split mode and the split part was recollected in the same tube and further analyzed by a different (flow modulated) comprehensive two‐dimensional gas chromatography system with a parallel detection, specifically using a quadrupole mass spectrometer and a vacuum ultraviolet detector. Both the comprehensive two‐dimensional gas chromatography platforms enabled the longitudinal tracking of the peppermint oil metabolites in exhaled breath. The increased sensitivity of comprehensive two‐dimensional gas chromatography enabled to successfully monitor over a 6.5 h period a total of 10 target compounds, namely α‐pinene, camphene, β‐pinene, limonene, cymene, eucalyptol, menthofuran, menthone, isomenthone, and neomenthol. John Wiley and Sons Inc. 2022-08-02 2022-09 /pmc/articles/PMC9804543/ /pubmed/35853166 http://dx.doi.org/10.1002/jssc.202200164 Text en © 2022 The Authors. Journal of Separation Science published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
spellingShingle Gas Chromatography
Zanella, Delphine
Henin, Adèle
Mascrez, Steven
Stefanuto, Pierre‐Hugues
Franchina, Flavio Antonio
Focant, Jean‐François
Purcaro, Giorgia
Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
title Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
title_full Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
title_fullStr Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
title_full_unstemmed Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
title_short Comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
title_sort comprehensive two‐dimensional gas chromatographic platforms comparison for exhaled breath metabolites analysis
topic Gas Chromatography
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804543/
https://www.ncbi.nlm.nih.gov/pubmed/35853166
http://dx.doi.org/10.1002/jssc.202200164
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