Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy

A bright and photostable fluorescent dye with a disulfide (S−S) linker and maleimide group (Rho594‐S2‐mal), as cleavable and reactive sites, was synthesized and conjugated with anti‐GFP nanobodies (NB). The binding of EGFP (FRET donor) with anti‐GFP NB labeled with one or two Rho594‐S2‐mal residues...

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Detalles Bibliográficos
Autores principales: Aktalay, Ayse, Ponsot, Flavien, Bossi, Mariano L., Belov, Vladimir N., Hell, Stefan W.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804610/
https://www.ncbi.nlm.nih.gov/pubmed/35838445
http://dx.doi.org/10.1002/cbic.202200395

Internet

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804610/
https://www.ncbi.nlm.nih.gov/pubmed/35838445
http://dx.doi.org/10.1002/cbic.202200395

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