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Cleavable Linker Incorporation into a Synthetic Dye‐Nanobody‐Fluorescent Protein Assembly: FRET, FLIM and STED Microscopy
A bright and photostable fluorescent dye with a disulfide (S−S) linker and maleimide group (Rho594‐S2‐mal), as cleavable and reactive sites, was synthesized and conjugated with anti‐GFP nanobodies (NB). The binding of EGFP (FRET donor) with anti‐GFP NB labeled with one or two Rho594‐S2‐mal residues...
| Autores principales: | , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
John Wiley and Sons Inc.
2022
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804610/ https://www.ncbi.nlm.nih.gov/pubmed/35838445 http://dx.doi.org/10.1002/cbic.202200395 |