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Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques

Heparan sulfate glycosaminoglycans provides extracellular matrix defense against heavy metals cytotoxicity. Identifying the precise glycan sequences that bind a particular heavy metal ion is a key for understanding those interactions. Here, electrochemical and surface characterization techniques wer...

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Autores principales: Shitrit, Ariel, Mardhekar, Sandhya, Alshanski, Israel, Jain, Prashant, Raigawali, Rakesh, Shanthamurthy, Chethan D., Kikkeri, Raghavendra, Yitzchaik, Shlomo, Hurevich, Mattan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804848/
https://www.ncbi.nlm.nih.gov/pubmed/35904207
http://dx.doi.org/10.1002/chem.202202193
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author Shitrit, Ariel
Mardhekar, Sandhya
Alshanski, Israel
Jain, Prashant
Raigawali, Rakesh
Shanthamurthy, Chethan D.
Kikkeri, Raghavendra
Yitzchaik, Shlomo
Hurevich, Mattan
author_facet Shitrit, Ariel
Mardhekar, Sandhya
Alshanski, Israel
Jain, Prashant
Raigawali, Rakesh
Shanthamurthy, Chethan D.
Kikkeri, Raghavendra
Yitzchaik, Shlomo
Hurevich, Mattan
author_sort Shitrit, Ariel
collection PubMed
description Heparan sulfate glycosaminoglycans provides extracellular matrix defense against heavy metals cytotoxicity. Identifying the precise glycan sequences that bind a particular heavy metal ion is a key for understanding those interactions. Here, electrochemical and surface characterization techniques were used to elucidate the relation between the glycans structural motifs, uronic acid stereochemistry, and sulfation regiochemistry to heavy metal ions binding. A divergent strategy was employed to access a small library of structurally well‐defined tetrasaccharides analogs with different sulfation patterns and uronic acid compositions. These tetrasaccharides were electrochemically grafted onto glassy carbon electrodes and their response to heavy metal ions was monitored by electrochemical impedance spectroscopy. Key differences in the binding of Hg(II), Cd(II), and Pb(II) were associated with a combination of the uronic acid type and the sulfation pattern.
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spelling pubmed-98048482023-01-06 Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques Shitrit, Ariel Mardhekar, Sandhya Alshanski, Israel Jain, Prashant Raigawali, Rakesh Shanthamurthy, Chethan D. Kikkeri, Raghavendra Yitzchaik, Shlomo Hurevich, Mattan Chemistry Research Articles Heparan sulfate glycosaminoglycans provides extracellular matrix defense against heavy metals cytotoxicity. Identifying the precise glycan sequences that bind a particular heavy metal ion is a key for understanding those interactions. Here, electrochemical and surface characterization techniques were used to elucidate the relation between the glycans structural motifs, uronic acid stereochemistry, and sulfation regiochemistry to heavy metal ions binding. A divergent strategy was employed to access a small library of structurally well‐defined tetrasaccharides analogs with different sulfation patterns and uronic acid compositions. These tetrasaccharides were electrochemically grafted onto glassy carbon electrodes and their response to heavy metal ions was monitored by electrochemical impedance spectroscopy. Key differences in the binding of Hg(II), Cd(II), and Pb(II) were associated with a combination of the uronic acid type and the sulfation pattern. John Wiley and Sons Inc. 2022-08-18 2022-10-04 /pmc/articles/PMC9804848/ /pubmed/35904207 http://dx.doi.org/10.1002/chem.202202193 Text en © 2022 The Authors. Chemistry - A European Journal published by Wiley-VCH GmbH https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Research Articles
Shitrit, Ariel
Mardhekar, Sandhya
Alshanski, Israel
Jain, Prashant
Raigawali, Rakesh
Shanthamurthy, Chethan D.
Kikkeri, Raghavendra
Yitzchaik, Shlomo
Hurevich, Mattan
Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques
title Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques
title_full Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques
title_fullStr Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques
title_full_unstemmed Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques
title_short Profiling Heparan Sulfate‐Heavy Metal Ions Interaction Using Electrochemical Techniques
title_sort profiling heparan sulfate‐heavy metal ions interaction using electrochemical techniques
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804848/
https://www.ncbi.nlm.nih.gov/pubmed/35904207
http://dx.doi.org/10.1002/chem.202202193
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