Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex

This article details the development of a single multiplex system amplifying 26 rapidly mutating Y‐STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y‐STRs commercial kits in diffe...

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Detalles Bibliográficos
Autores principales: Albastaki, Abdulla, Naji, Mohammed, Lootah, Reem, Almheiri, Reem, Almulla, Hanan, Alreyami, Afra, Almarri, Iman, Yu, Zailiang, Zhang, Yueke, Shu, Chang, Alghafri, Rashed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Regular Part
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804972/
https://www.ncbi.nlm.nih.gov/pubmed/35899438
http://dx.doi.org/10.1002/elps.202200099
Descripción
Sumario:This article details the development of a single multiplex system amplifying 26 rapidly mutating Y‐STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y‐STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.

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