Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex

This article details the development of a single multiplex system amplifying 26 rapidly mutating Y‐STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y‐STRs commercial kits in diffe...

Descripción completa

Detalles Bibliográficos
Autores principales: Albastaki, Abdulla, Naji, Mohammed, Lootah, Reem, Almheiri, Reem, Almulla, Hanan, Alreyami, Afra, Almarri, Iman, Yu, Zailiang, Zhang, Yueke, Shu, Chang, Alghafri, Rashed
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2022
Materias:
Regular Part
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804972/
https://www.ncbi.nlm.nih.gov/pubmed/35899438
http://dx.doi.org/10.1002/elps.202200099
_version_ 1784862235889238016
author Albastaki, Abdulla
Naji, Mohammed
Lootah, Reem
Almheiri, Reem
Almulla, Hanan
Alreyami, Afra
Almarri, Iman
Yu, Zailiang
Zhang, Yueke
Shu, Chang
Alghafri, Rashed
author_facet Albastaki, Abdulla
Naji, Mohammed
Lootah, Reem
Almheiri, Reem
Almulla, Hanan
Alreyami, Afra
Almarri, Iman
Yu, Zailiang
Zhang, Yueke
Shu, Chang
Alghafri, Rashed
author_sort Albastaki, Abdulla
collection PubMed
description This article details the development of a single multiplex system amplifying 26 rapidly mutating Y‐STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y‐STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework.
format Online
Article
Text
id pubmed-9804972
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher John Wiley and Sons Inc.
record_format MEDLINE/PubMed
spelling pubmed-98049722023-01-06 Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex Albastaki, Abdulla Naji, Mohammed Lootah, Reem Almheiri, Reem Almulla, Hanan Alreyami, Afra Almarri, Iman Yu, Zailiang Zhang, Yueke Shu, Chang Alghafri, Rashed Electrophoresis Regular Part This article details the development of a single multiplex system amplifying 26 rapidly mutating Y‐STR markers. A sequenced allelic ladder, constructed for calling alleles of all loci, is introduced. The multiplex system shows the ability to address the limitations of Y‐STRs commercial kits in differentiating closely related males. The multiplex performed well in the prevalidation tests and showed great potential to be used in forensic casework. John Wiley and Sons Inc. 2022-08-08 2022-10 /pmc/articles/PMC9804972/ /pubmed/35899438 http://dx.doi.org/10.1002/elps.202200099 Text en © 2022 The Authors. Electrophoresis published by Wiley‐VCH GmbH. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nc-nd/4.0/ (https://creativecommons.org/licenses/by-nc-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non‐commercial and no modifications or adaptations are made.
spellingShingle Regular Part
Albastaki, Abdulla
Naji, Mohammed
Lootah, Reem
Almheiri, Reem
Almulla, Hanan
Alreyami, Afra
Almarri, Iman
Yu, Zailiang
Zhang, Yueke
Shu, Chang
Alghafri, Rashed
Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex
title Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex
title_full Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex
title_fullStr Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex
title_full_unstemmed Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex
title_short Design and development of novel single multiplex system incorporating 26 rapidly mutating Y‐STRs; 26 RM Yplex
title_sort design and development of novel single multiplex system incorporating 26 rapidly mutating y‐strs; 26 rm yplex
topic Regular Part
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9804972/
https://www.ncbi.nlm.nih.gov/pubmed/35899438
http://dx.doi.org/10.1002/elps.202200099
work_keys_str_mv AT albastakiabdulla designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT najimohammed designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT lootahreem designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT almheirireem designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT almullahanan designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT alreyamiafra designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT almarriiman designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT yuzailiang designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT zhangyueke designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT shuchang designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex
AT alghafrirashed designanddevelopmentofnovelsinglemultiplexsystemincorporating26rapidlymutatingystrs26rmyplex

Ejemplares similares