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Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure

Carbonate apatite (CO(3)Ap) granules are useful as a bone substitute because they can be remodeled to new natural bone in a manner that conforms to the bone remodeling process. However, reconstructing large bone defects using CO(3)Ap granules is difficult because of their granular shape. Therefore,...

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Autores principales: Kudoh, Keiko, Fukuda, Naoyuki, Akita, Kazuya, Kudoh, Takaharu, Takamaru, Natsumi, Kurio, Naito, Hayashi, Koichiro, Ishikawa, Kunio, Miyamoto, Youji
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9805415/
https://www.ncbi.nlm.nih.gov/pubmed/36586041
http://dx.doi.org/10.1007/s10856-022-06710-2
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author Kudoh, Keiko
Fukuda, Naoyuki
Akita, Kazuya
Kudoh, Takaharu
Takamaru, Natsumi
Kurio, Naito
Hayashi, Koichiro
Ishikawa, Kunio
Miyamoto, Youji
author_facet Kudoh, Keiko
Fukuda, Naoyuki
Akita, Kazuya
Kudoh, Takaharu
Takamaru, Natsumi
Kurio, Naito
Hayashi, Koichiro
Ishikawa, Kunio
Miyamoto, Youji
author_sort Kudoh, Keiko
collection PubMed
description Carbonate apatite (CO(3)Ap) granules are useful as a bone substitute because they can be remodeled to new natural bone in a manner that conforms to the bone remodeling process. However, reconstructing large bone defects using CO(3)Ap granules is difficult because of their granular shape. Therefore, we fabricated CO(3)Ap honeycomb blocks (HCBs) with continuous unidirectional pores. We aimed to elucidate the tissue response and availability of CO(3)Ap HCBs in the reconstruction of rabbit mandibular bone defects after marginal mandibulectomy. The percentages of the remaining CO(3)Ap area and calcified bone area (newly formed bone) were estimated from the histological images. CO(3)Ap area was 49.1 ± 4.9%, 30.3 ± 3.5%, and 25.5 ± 8.8%, whereas newly formed bone area was 3.0 ± 0.6%, 24.3 ± 3.3%, and 34.7 ± 4.8% at 4, 8, and 12 weeks, respectively, after implantation. Thus, CO(3)Ap HCBs were gradually resorbed and replaced by new bone. The newly formed bone penetrated most of the pores in the CO(3)Ap HCBs at 12 weeks after implantation. By contrast, the granulation tissue scarcely invaded the CO(3)Ap HCBs. Some osteoclasts invaded the wall of CO(3)Ap HCBs, making resorption pits. Furthermore, many osteoblasts were found on the newly formed bone, indicating ongoing bone remodeling. Blood vessels were also formed inside most of the pores in the CO(3)Ap HCBs. These findings suggest that CO(3)Ap HCBs have good osteoconductivity and can be used for the reconstruction of large mandibular bone defects. GRAPHICAL ABSTRACT: [Figure: see text]
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spelling pubmed-98054152023-01-02 Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure Kudoh, Keiko Fukuda, Naoyuki Akita, Kazuya Kudoh, Takaharu Takamaru, Natsumi Kurio, Naito Hayashi, Koichiro Ishikawa, Kunio Miyamoto, Youji J Mater Sci Mater Med Biocompatibility Studies Carbonate apatite (CO(3)Ap) granules are useful as a bone substitute because they can be remodeled to new natural bone in a manner that conforms to the bone remodeling process. However, reconstructing large bone defects using CO(3)Ap granules is difficult because of their granular shape. Therefore, we fabricated CO(3)Ap honeycomb blocks (HCBs) with continuous unidirectional pores. We aimed to elucidate the tissue response and availability of CO(3)Ap HCBs in the reconstruction of rabbit mandibular bone defects after marginal mandibulectomy. The percentages of the remaining CO(3)Ap area and calcified bone area (newly formed bone) were estimated from the histological images. CO(3)Ap area was 49.1 ± 4.9%, 30.3 ± 3.5%, and 25.5 ± 8.8%, whereas newly formed bone area was 3.0 ± 0.6%, 24.3 ± 3.3%, and 34.7 ± 4.8% at 4, 8, and 12 weeks, respectively, after implantation. Thus, CO(3)Ap HCBs were gradually resorbed and replaced by new bone. The newly formed bone penetrated most of the pores in the CO(3)Ap HCBs at 12 weeks after implantation. By contrast, the granulation tissue scarcely invaded the CO(3)Ap HCBs. Some osteoclasts invaded the wall of CO(3)Ap HCBs, making resorption pits. Furthermore, many osteoblasts were found on the newly formed bone, indicating ongoing bone remodeling. Blood vessels were also formed inside most of the pores in the CO(3)Ap HCBs. These findings suggest that CO(3)Ap HCBs have good osteoconductivity and can be used for the reconstruction of large mandibular bone defects. GRAPHICAL ABSTRACT: [Figure: see text] Springer US 2022-12-31 2023 /pmc/articles/PMC9805415/ /pubmed/36586041 http://dx.doi.org/10.1007/s10856-022-06710-2 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Biocompatibility Studies
Kudoh, Keiko
Fukuda, Naoyuki
Akita, Kazuya
Kudoh, Takaharu
Takamaru, Natsumi
Kurio, Naito
Hayashi, Koichiro
Ishikawa, Kunio
Miyamoto, Youji
Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
title Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
title_full Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
title_fullStr Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
title_full_unstemmed Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
title_short Reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
title_sort reconstruction of rabbit mandibular bone defects using carbonate apatite honeycomb blocks with an interconnected porous structure
topic Biocompatibility Studies
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9805415/
https://www.ncbi.nlm.nih.gov/pubmed/36586041
http://dx.doi.org/10.1007/s10856-022-06710-2
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