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Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species

Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such pa...

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Autores principales: Kiemel, K., Gurke, M., Paraskevopoulou, S., Havenstein, K., Weithoff, G., Tiedemann, R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9805463/
https://www.ncbi.nlm.nih.gov/pubmed/36587065
http://dx.doi.org/10.1038/s41598-022-27137-3
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author Kiemel, K.
Gurke, M.
Paraskevopoulou, S.
Havenstein, K.
Weithoff, G.
Tiedemann, R.
author_facet Kiemel, K.
Gurke, M.
Paraskevopoulou, S.
Havenstein, K.
Weithoff, G.
Tiedemann, R.
author_sort Kiemel, K.
collection PubMed
description Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species.
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spelling pubmed-98054632023-01-02 Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species Kiemel, K. Gurke, M. Paraskevopoulou, S. Havenstein, K. Weithoff, G. Tiedemann, R. Sci Rep Article Genetic divergence and the frequency of hybridization are central for defining species delimitations, especially among cryptic species where morphological differences are merely absent. Rotifers are known for their high cryptic diversity and therefore are ideal model organisms to investigate such patterns. Here, we used the recently resolved Brachionus calyciflorus species complex to investigate whether previously observed between species differences in thermotolerance and gene expression are also reflected in their genomic footprint. We identified a Heat Shock Protein gene (HSP 40 kDa) which exhibits cross species pronounced sequence variation. This gene exhibits species-specific fixed sites, alleles, and sites putatively under positive selection. These sites are located in protein binding regions involved in chaperoning and may therefore reflect adaptive diversification. By comparing three genetic markers (ITS, COI, HSP 40 kDa), we revealed hybridization events between the cryptic species. The low frequency of introgressive haplotypes/alleles suggest a tight, but not fully impermeable boundary between the cryptic species. Nature Publishing Group UK 2022-12-31 /pmc/articles/PMC9805463/ /pubmed/36587065 http://dx.doi.org/10.1038/s41598-022-27137-3 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Kiemel, K.
Gurke, M.
Paraskevopoulou, S.
Havenstein, K.
Weithoff, G.
Tiedemann, R.
Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species
title Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species
title_full Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species
title_fullStr Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species
title_full_unstemmed Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species
title_short Variation in heat shock protein 40 kDa relates to divergence in thermotolerance among cryptic rotifer species
title_sort variation in heat shock protein 40 kda relates to divergence in thermotolerance among cryptic rotifer species
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9805463/
https://www.ncbi.nlm.nih.gov/pubmed/36587065
http://dx.doi.org/10.1038/s41598-022-27137-3
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