A Novel CRISPR Interference Effector Enabling Functional Gene Characterization with Synthetic Guide RNAs

While CRISPR interference (CRISPRi) systems have been widely implemented in pooled lentiviral screening, there has been limited use with synthetic guide RNAs for the complex phenotypic readouts enabled by experiments in arrayed format. Here we describe a novel deactivated Cas9 fusion protein, dCas9-...

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Detalles Bibliográficos
Autores principales: Mills, Clarence, Riching, Andrew, Keller, Ashleigh, Stombaugh, Jesse, Haupt, Amanda, Maksimova, Elena, Dickerson, Sarah M., Anderson, Emily, Hemphill, Kevin, Ebmeier, Chris, Schiel, John A., Levenga, Josien, Perkett, Matthew, Smith, Anja van Brabant, Strezoska, Zaklina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Mary Ann Liebert, Inc., publishers 2022
Materias:
Research Articles
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9805873/
https://www.ncbi.nlm.nih.gov/pubmed/36257604
http://dx.doi.org/10.1089/crispr.2022.0056

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