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Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner

OBJECTIVES: Sweet bee venom (sBV) is purified from Apis mellifera, containing a high level of melittin—its main component. It has been used as a therapeutic agent for pain relief and anti-inflammation, as well as for treating neuronal abnormalities. Recently, there have been studies on the therapeut...

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Autores principales: Hwang, Yu-Na, Kwon, In-Seo, Na, Han-Heom, Park, Jin-Sung, Kim, Keun-Cheol
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Pharmacopuncture Institute (KPI) 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806155/
https://www.ncbi.nlm.nih.gov/pubmed/36628342
http://dx.doi.org/10.3831/KPI.2022.25.4.390
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author Hwang, Yu-Na
Kwon, In-Seo
Na, Han-Heom
Park, Jin-Sung
Kim, Keun-Cheol
author_facet Hwang, Yu-Na
Kwon, In-Seo
Na, Han-Heom
Park, Jin-Sung
Kim, Keun-Cheol
author_sort Hwang, Yu-Na
collection PubMed
description OBJECTIVES: Sweet bee venom (sBV) is purified from Apis mellifera, containing a high level of melittin—its main component. It has been used as a therapeutic agent for pain relief and anti-inflammation, as well as for treating neuronal abnormalities. Recently, there have been studies on the therapeutic application of sBV for anticancer treatment. In the present study, we investigated the pharmacological effect of sBV treatment in A549 human lung cancer cells. METHODS: We used microscopic analysis to observe the morphological changes in A549 cells after sBV treatment. The MTT assay was used to examine the cytotoxic effect after dose-dependent sBV treatment. Molecular changes in sBV were evaluated by the expression of apoptosis marker proteins using western blot analysis. RESULTS: Microscopic analysis suggested that the growth inhibitory effect occurred in a dose-dependent manner; however, cell lysis occurred at a concentration over 20 μg/mL of sBV. The MTT assay indicated that sBV treatment exhibited a growth inhibitory effect at a concentration over 5 μg/mL. On fluorescence activated cell sorting analysis, G0 dead cells were observed after G1 arrest at treatment concentrations up to 10 μg/mL. However, rapid cell rupture was observed at a concentration of 20 μg/mL. Western blot analysis demonstrated that sBV treatment modulated the expression of multiple cell death-related proteins, including cleaved-PARP, cleaved-caspase 9, p53, Bcl2, and Bax. CONCLUSION: sBV induced cell death in A549 human lung cancer cells at a pharmacological concentration, albeit causing hemolytic cell death at a high concentration.
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spelling pubmed-98061552023-01-09 Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner Hwang, Yu-Na Kwon, In-Seo Na, Han-Heom Park, Jin-Sung Kim, Keun-Cheol J Pharmacopuncture Original Article OBJECTIVES: Sweet bee venom (sBV) is purified from Apis mellifera, containing a high level of melittin—its main component. It has been used as a therapeutic agent for pain relief and anti-inflammation, as well as for treating neuronal abnormalities. Recently, there have been studies on the therapeutic application of sBV for anticancer treatment. In the present study, we investigated the pharmacological effect of sBV treatment in A549 human lung cancer cells. METHODS: We used microscopic analysis to observe the morphological changes in A549 cells after sBV treatment. The MTT assay was used to examine the cytotoxic effect after dose-dependent sBV treatment. Molecular changes in sBV were evaluated by the expression of apoptosis marker proteins using western blot analysis. RESULTS: Microscopic analysis suggested that the growth inhibitory effect occurred in a dose-dependent manner; however, cell lysis occurred at a concentration over 20 μg/mL of sBV. The MTT assay indicated that sBV treatment exhibited a growth inhibitory effect at a concentration over 5 μg/mL. On fluorescence activated cell sorting analysis, G0 dead cells were observed after G1 arrest at treatment concentrations up to 10 μg/mL. However, rapid cell rupture was observed at a concentration of 20 μg/mL. Western blot analysis demonstrated that sBV treatment modulated the expression of multiple cell death-related proteins, including cleaved-PARP, cleaved-caspase 9, p53, Bcl2, and Bax. CONCLUSION: sBV induced cell death in A549 human lung cancer cells at a pharmacological concentration, albeit causing hemolytic cell death at a high concentration. The Korean Pharmacopuncture Institute (KPI) 2022-12-31 2022-12-31 /pmc/articles/PMC9806155/ /pubmed/36628342 http://dx.doi.org/10.3831/KPI.2022.25.4.390 Text en © 2022 Korean Pharmacopuncture Institute https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0 (https://creativecommons.org/licenses/by-nc/4.0/) ) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Hwang, Yu-Na
Kwon, In-Seo
Na, Han-Heom
Park, Jin-Sung
Kim, Keun-Cheol
Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner
title Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner
title_full Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner
title_fullStr Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner
title_full_unstemmed Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner
title_short Dual Cytotoxic Responses Induced by Treatment of A549 Human Lung Cancer Cells with Sweet Bee Venom in a Dose-Dependent Manner
title_sort dual cytotoxic responses induced by treatment of a549 human lung cancer cells with sweet bee venom in a dose-dependent manner
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806155/
https://www.ncbi.nlm.nih.gov/pubmed/36628342
http://dx.doi.org/10.3831/KPI.2022.25.4.390
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