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The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19
Patients infected by the SARS-CoV-2 virus are commonly diagnosed with threatening liver conditions associated with drug-induced therapies and systemic viral action. RNA-Seq data from cells in bronchoalveolar lavage fluid from COVID-19 patients have pointed out dysregulation of kallikrein-kinin and r...
Autores principales: | , , , , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2022
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806358/ https://www.ncbi.nlm.nih.gov/pubmed/36601349 http://dx.doi.org/10.3389/fphys.2022.1080837 |
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author | Mendes, Gabriel Moreira de M Do Nascimento, Israel Júnior Borges Marazzi-Diniz, Paulo HS. Da Silveira, Izabela B. Itaborahy, Matheus F. Viana, Luiz E. Silva, Filipe A. Santana, Monique F Pinto, Rebecca AA. Dutra, Bruna G. Lacerda, Marcus Vinicius G. Araujo, Stanley A. Wanderley, David Vidigal, Paula VT. Diniz, Paulo HC Verano-Braga, Thiago Santos, Robson AS. Leite, M Fatima |
author_facet | Mendes, Gabriel Moreira de M Do Nascimento, Israel Júnior Borges Marazzi-Diniz, Paulo HS. Da Silveira, Izabela B. Itaborahy, Matheus F. Viana, Luiz E. Silva, Filipe A. Santana, Monique F Pinto, Rebecca AA. Dutra, Bruna G. Lacerda, Marcus Vinicius G. Araujo, Stanley A. Wanderley, David Vidigal, Paula VT. Diniz, Paulo HC Verano-Braga, Thiago Santos, Robson AS. Leite, M Fatima |
author_sort | Mendes, Gabriel Moreira de M |
collection | PubMed |
description | Patients infected by the SARS-CoV-2 virus are commonly diagnosed with threatening liver conditions associated with drug-induced therapies and systemic viral action. RNA-Seq data from cells in bronchoalveolar lavage fluid from COVID-19 patients have pointed out dysregulation of kallikrein-kinin and renin-angiotensin systems as a possible mechanism that triggers multi-organ damage away from the leading site of virus infection. Therefore, we measured the plasma concentration of biologically active peptides from the kallikrein-kinin system, bradykinin and des-Arg(9)-bradykinin, and liver expression of its proinflammatory axis, bradykinin 1 receptor (B1R). We measured the plasma concentration of bradykinin and des-Arg(9)-bradykinin of 20 virologically confirmed COVID-19 patients using a liquid chromatography-tandem mass spectrometry-based methodology. The expression of B1R was evaluated by immunohistochemistry from post-mortem liver specimens of 27 COVID-19 individuals. We found a significantly higher blood level of des-Arg(9)-bradykinin and a lower bradykinin concentration in patients with COVID-19 compared to a healthy, uninfected control group. We also observed increased B1R expression levels in hepatic tissues of patients with COVID-19 under all hepatic injuries analyzed (liver congestion, portal vein dilation, steatosis, and ischemic necrosis). Our data indicate that des-Arg(9)-bradykinin/B1R is associated with the acute hepatic dysfunction induced by the SARS-CoV-2 virus infection in the pathogenesis of COVID-19. |
format | Online Article Text |
id | pubmed-9806358 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-98063582023-01-03 The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 Mendes, Gabriel Moreira de M Do Nascimento, Israel Júnior Borges Marazzi-Diniz, Paulo HS. Da Silveira, Izabela B. Itaborahy, Matheus F. Viana, Luiz E. Silva, Filipe A. Santana, Monique F Pinto, Rebecca AA. Dutra, Bruna G. Lacerda, Marcus Vinicius G. Araujo, Stanley A. Wanderley, David Vidigal, Paula VT. Diniz, Paulo HC Verano-Braga, Thiago Santos, Robson AS. Leite, M Fatima Front Physiol Physiology Patients infected by the SARS-CoV-2 virus are commonly diagnosed with threatening liver conditions associated with drug-induced therapies and systemic viral action. RNA-Seq data from cells in bronchoalveolar lavage fluid from COVID-19 patients have pointed out dysregulation of kallikrein-kinin and renin-angiotensin systems as a possible mechanism that triggers multi-organ damage away from the leading site of virus infection. Therefore, we measured the plasma concentration of biologically active peptides from the kallikrein-kinin system, bradykinin and des-Arg(9)-bradykinin, and liver expression of its proinflammatory axis, bradykinin 1 receptor (B1R). We measured the plasma concentration of bradykinin and des-Arg(9)-bradykinin of 20 virologically confirmed COVID-19 patients using a liquid chromatography-tandem mass spectrometry-based methodology. The expression of B1R was evaluated by immunohistochemistry from post-mortem liver specimens of 27 COVID-19 individuals. We found a significantly higher blood level of des-Arg(9)-bradykinin and a lower bradykinin concentration in patients with COVID-19 compared to a healthy, uninfected control group. We also observed increased B1R expression levels in hepatic tissues of patients with COVID-19 under all hepatic injuries analyzed (liver congestion, portal vein dilation, steatosis, and ischemic necrosis). Our data indicate that des-Arg(9)-bradykinin/B1R is associated with the acute hepatic dysfunction induced by the SARS-CoV-2 virus infection in the pathogenesis of COVID-19. Frontiers Media S.A. 2022-12-19 /pmc/articles/PMC9806358/ /pubmed/36601349 http://dx.doi.org/10.3389/fphys.2022.1080837 Text en Copyright © 2022 Mendes, Do Nascimento, Marazzi-Diniz, Da Silveira, Itaborahy, Viana, Silva, Santana, Pinto, Dutra, Lacerda, Araujo, Wanderley, Vidigal, Diniz, Verano-Braga, Santos and Leite. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Physiology Mendes, Gabriel Moreira de M Do Nascimento, Israel Júnior Borges Marazzi-Diniz, Paulo HS. Da Silveira, Izabela B. Itaborahy, Matheus F. Viana, Luiz E. Silva, Filipe A. Santana, Monique F Pinto, Rebecca AA. Dutra, Bruna G. Lacerda, Marcus Vinicius G. Araujo, Stanley A. Wanderley, David Vidigal, Paula VT. Diniz, Paulo HC Verano-Braga, Thiago Santos, Robson AS. Leite, M Fatima The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 |
title | The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 |
title_full | The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 |
title_fullStr | The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 |
title_full_unstemmed | The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 |
title_short | The des-Arg(9)-bradykinin/B1R axis: Hepatic damage in COVID-19 |
title_sort | des-arg(9)-bradykinin/b1r axis: hepatic damage in covid-19 |
topic | Physiology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806358/ https://www.ncbi.nlm.nih.gov/pubmed/36601349 http://dx.doi.org/10.3389/fphys.2022.1080837 |
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