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PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection

T cell-B cell interaction is the key immune response to protect the host from severe viral infection. However, how T cells support B cells to exert protective humoral immunity in humans is not well understood. Here, we use COVID-19 as a model of acute viral infections and analyze CD4(+) T cell subse...

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Autores principales: Asashima, Hiromitsu, Mohanty, Subhasis, Comi, Michela, Ruff, William E., Hoehn, Kenneth B., Wong, Patrick, Klein, Jon, Lucas, Carolina, Cohen, Inessa, Coffey, Sarah, Lele, Nikhil, Greta, Leissa, Raddassi, Khadir, Chaudhary, Omkar, Unterman, Avraham, Emu, Brinda, Kleinstein, Steven H., Montgomery, Ruth R., Iwasaki, Akiko, Dela Cruz, Charles S., Kaminski, Naftali, Shaw, Albert C., Hafler, David A., Sumida, Tomokazu S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Authors. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806868/
https://www.ncbi.nlm.nih.gov/pubmed/36596303
http://dx.doi.org/10.1016/j.celrep.2022.111895
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author Asashima, Hiromitsu
Mohanty, Subhasis
Comi, Michela
Ruff, William E.
Hoehn, Kenneth B.
Wong, Patrick
Klein, Jon
Lucas, Carolina
Cohen, Inessa
Coffey, Sarah
Lele, Nikhil
Greta, Leissa
Raddassi, Khadir
Chaudhary, Omkar
Unterman, Avraham
Emu, Brinda
Kleinstein, Steven H.
Montgomery, Ruth R.
Iwasaki, Akiko
Dela Cruz, Charles S.
Kaminski, Naftali
Shaw, Albert C.
Hafler, David A.
Sumida, Tomokazu S.
author_facet Asashima, Hiromitsu
Mohanty, Subhasis
Comi, Michela
Ruff, William E.
Hoehn, Kenneth B.
Wong, Patrick
Klein, Jon
Lucas, Carolina
Cohen, Inessa
Coffey, Sarah
Lele, Nikhil
Greta, Leissa
Raddassi, Khadir
Chaudhary, Omkar
Unterman, Avraham
Emu, Brinda
Kleinstein, Steven H.
Montgomery, Ruth R.
Iwasaki, Akiko
Dela Cruz, Charles S.
Kaminski, Naftali
Shaw, Albert C.
Hafler, David A.
Sumida, Tomokazu S.
author_sort Asashima, Hiromitsu
collection PubMed
description T cell-B cell interaction is the key immune response to protect the host from severe viral infection. However, how T cells support B cells to exert protective humoral immunity in humans is not well understood. Here, we use COVID-19 as a model of acute viral infections and analyze CD4(+) T cell subsets associated with plasmablast expansion and clinical outcome. Peripheral helper T cells (Tph cells; denoted as PD-1(high)CXCR5(–)CD4(+) T cells) are significantly increased, as are plasmablasts. Tph cells exhibit “B cell help” signatures and induce plasmablast differentiation in vitro. Interestingly, expanded plasmablasts show increased CXCR3 expression, which is positively correlated with higher frequency of activated Tph cells and better clinical outcome. Mechanistically, Tph cells help B cell differentiation and produce more interferon γ (IFNγ), which induces CXCR3 expression on plasmablasts. These results elucidate a role for Tph cells in regulating protective B cell response during acute viral infection.
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spelling pubmed-98068682023-01-04 PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection Asashima, Hiromitsu Mohanty, Subhasis Comi, Michela Ruff, William E. Hoehn, Kenneth B. Wong, Patrick Klein, Jon Lucas, Carolina Cohen, Inessa Coffey, Sarah Lele, Nikhil Greta, Leissa Raddassi, Khadir Chaudhary, Omkar Unterman, Avraham Emu, Brinda Kleinstein, Steven H. Montgomery, Ruth R. Iwasaki, Akiko Dela Cruz, Charles S. Kaminski, Naftali Shaw, Albert C. Hafler, David A. Sumida, Tomokazu S. Cell Rep Report T cell-B cell interaction is the key immune response to protect the host from severe viral infection. However, how T cells support B cells to exert protective humoral immunity in humans is not well understood. Here, we use COVID-19 as a model of acute viral infections and analyze CD4(+) T cell subsets associated with plasmablast expansion and clinical outcome. Peripheral helper T cells (Tph cells; denoted as PD-1(high)CXCR5(–)CD4(+) T cells) are significantly increased, as are plasmablasts. Tph cells exhibit “B cell help” signatures and induce plasmablast differentiation in vitro. Interestingly, expanded plasmablasts show increased CXCR3 expression, which is positively correlated with higher frequency of activated Tph cells and better clinical outcome. Mechanistically, Tph cells help B cell differentiation and produce more interferon γ (IFNγ), which induces CXCR3 expression on plasmablasts. These results elucidate a role for Tph cells in regulating protective B cell response during acute viral infection. The Authors. 2023-01-31 2023-01-02 /pmc/articles/PMC9806868/ /pubmed/36596303 http://dx.doi.org/10.1016/j.celrep.2022.111895 Text en © 2022 The Authors Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.
spellingShingle Report
Asashima, Hiromitsu
Mohanty, Subhasis
Comi, Michela
Ruff, William E.
Hoehn, Kenneth B.
Wong, Patrick
Klein, Jon
Lucas, Carolina
Cohen, Inessa
Coffey, Sarah
Lele, Nikhil
Greta, Leissa
Raddassi, Khadir
Chaudhary, Omkar
Unterman, Avraham
Emu, Brinda
Kleinstein, Steven H.
Montgomery, Ruth R.
Iwasaki, Akiko
Dela Cruz, Charles S.
Kaminski, Naftali
Shaw, Albert C.
Hafler, David A.
Sumida, Tomokazu S.
PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
title PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
title_full PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
title_fullStr PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
title_full_unstemmed PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
title_short PD-1(high)CXCR5(–)CD4(+) peripheral helper T cells promote CXCR3(+) plasmablasts in human acute viral infection
title_sort pd-1(high)cxcr5(–)cd4(+) peripheral helper t cells promote cxcr3(+) plasmablasts in human acute viral infection
topic Report
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806868/
https://www.ncbi.nlm.nih.gov/pubmed/36596303
http://dx.doi.org/10.1016/j.celrep.2022.111895
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