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Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus
Polyploidy and the microbiome are crucial factors in how a host organism responds to disease. However, little is known about how triploidization and microbiome affect the immune response and disease resistance in the fish host. Therefore, this study aims to identify the relationship between intestin...
Autores principales: | , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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BioMed Central
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806896/ https://www.ncbi.nlm.nih.gov/pubmed/36593453 http://dx.doi.org/10.1186/s12866-022-02709-5 |
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author | Cai, Yidan Wei, Ke |
author_facet | Cai, Yidan Wei, Ke |
author_sort | Cai, Yidan |
collection | PubMed |
description | Polyploidy and the microbiome are crucial factors in how a host organism responds to disease. However, little is known about how triploidization and microbiome affect the immune response and disease resistance in the fish host. Therefore, this study aims to identify the relationship between intestinal microbiota composition, transcriptome changes, and disease resistance in triploid Carassius auratus (3nCC). In China’s central Dongting lake water system, diploid (2nCC) and triploid Carassius auratus were collected, then 16S rRNA and mRNA sequencing were used to examine the microbes and gene expression in the intestines. 16S rRNA sequencing demonstrated that triploidization altered intestinal richness, as well as the diversity of commensal bacteria in 3nCC. In addition, the abundance of the genus Vibrio in 3nCC was increased compared to 2nCC (P < 0.05). Furthermore, differential expression analysis of 3nCC revealed profound up-regulation of 293 transcripts, while 324 were down-regulated. Several differentially expressed transcripts were related to the immune response pathway in 3nCC, including NLRP3, LY9, PNMA1, MR1, PELI1, NOTCH2, NFIL3, and NLRC4. Taken together, triploidization can alter bacteria composition and abundance, which can in turn result in changes in expression of genes. This study offers an opportunity for deciphering the molecular mechanism underlying disease resistance after triploidization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02709-5. |
format | Online Article Text |
id | pubmed-9806896 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-98068962023-01-03 Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus Cai, Yidan Wei, Ke BMC Microbiol Research Polyploidy and the microbiome are crucial factors in how a host organism responds to disease. However, little is known about how triploidization and microbiome affect the immune response and disease resistance in the fish host. Therefore, this study aims to identify the relationship between intestinal microbiota composition, transcriptome changes, and disease resistance in triploid Carassius auratus (3nCC). In China’s central Dongting lake water system, diploid (2nCC) and triploid Carassius auratus were collected, then 16S rRNA and mRNA sequencing were used to examine the microbes and gene expression in the intestines. 16S rRNA sequencing demonstrated that triploidization altered intestinal richness, as well as the diversity of commensal bacteria in 3nCC. In addition, the abundance of the genus Vibrio in 3nCC was increased compared to 2nCC (P < 0.05). Furthermore, differential expression analysis of 3nCC revealed profound up-regulation of 293 transcripts, while 324 were down-regulated. Several differentially expressed transcripts were related to the immune response pathway in 3nCC, including NLRP3, LY9, PNMA1, MR1, PELI1, NOTCH2, NFIL3, and NLRC4. Taken together, triploidization can alter bacteria composition and abundance, which can in turn result in changes in expression of genes. This study offers an opportunity for deciphering the molecular mechanism underlying disease resistance after triploidization. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-022-02709-5. BioMed Central 2023-01-02 /pmc/articles/PMC9806896/ /pubmed/36593453 http://dx.doi.org/10.1186/s12866-022-02709-5 Text en © The Author(s) 2022 https://creativecommons.org/licenses/by/4.0/Open AccessThis article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data. |
spellingShingle | Research Cai, Yidan Wei, Ke Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus |
title | Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus |
title_full | Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus |
title_fullStr | Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus |
title_full_unstemmed | Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus |
title_short | Comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid Carassius auratus |
title_sort | comparative analysis of intestinal microbiota composition and transcriptome in diploid and triploid carassius auratus |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9806896/ https://www.ncbi.nlm.nih.gov/pubmed/36593453 http://dx.doi.org/10.1186/s12866-022-02709-5 |
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