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Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss

Osteoporosis is a common metabolic bone disease with a rapidly increasing prevalence, characterized by massive bone loss because of excessive osteoclast formation. Gallic acid (GA), a phenolic acid isolated from Cornus officinalis, has anti-inflammatory and anti-oxidant effects, but its effect on os...

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Autores principales: Zhang, Peng, Ye, Jiekai, Dai, Jiale, Wang, Ying, Chen, Genjun, Hu, Jinping, Hu, Qimiao, Fei, Jun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807166/
https://www.ncbi.nlm.nih.gov/pubmed/36601012
http://dx.doi.org/10.3389/fendo.2022.963237
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author Zhang, Peng
Ye, Jiekai
Dai, Jiale
Wang, Ying
Chen, Genjun
Hu, Jinping
Hu, Qimiao
Fei, Jun
author_facet Zhang, Peng
Ye, Jiekai
Dai, Jiale
Wang, Ying
Chen, Genjun
Hu, Jinping
Hu, Qimiao
Fei, Jun
author_sort Zhang, Peng
collection PubMed
description Osteoporosis is a common metabolic bone disease with a rapidly increasing prevalence, characterized by massive bone loss because of excessive osteoclast formation. Gallic acid (GA), a phenolic acid isolated from Cornus officinalis, has anti-inflammatory and anti-oxidant effects, but its effect on osteoclast formation has not been confirmed. In our study, we demonstrated that GA significantly inhibited RANKL‐induced osteoclast formation and function of osteoclast in bone marrow monocytes (BMMs) and RAW264.7 cells in a dose-dependent manner without cytotoxicity. For molecular mechanisms, GA repressed osteoclastogenesis by blocking Akt, ERK, and JNK pathways, and suppressed osteoclastogenesis-related marker expression, including nuclear factor of the activated T-cell cytoplasmic 1 (NFATc1), c‐Fos, and cathepsin K (CTSK). In addition, we further assessed the effect of GA in an ovariectomized mouse model, which indicated that GA has a notable effect on preventing bone loss. In conclusion, GA exerts notable effects in inhibiting osteoclastogenesis and preventing ovariectomy-induced bone loss, suggesting that GA is a potential agent in osteoporosis treatment.
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spelling pubmed-98071662023-01-03 Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss Zhang, Peng Ye, Jiekai Dai, Jiale Wang, Ying Chen, Genjun Hu, Jinping Hu, Qimiao Fei, Jun Front Endocrinol (Lausanne) Endocrinology Osteoporosis is a common metabolic bone disease with a rapidly increasing prevalence, characterized by massive bone loss because of excessive osteoclast formation. Gallic acid (GA), a phenolic acid isolated from Cornus officinalis, has anti-inflammatory and anti-oxidant effects, but its effect on osteoclast formation has not been confirmed. In our study, we demonstrated that GA significantly inhibited RANKL‐induced osteoclast formation and function of osteoclast in bone marrow monocytes (BMMs) and RAW264.7 cells in a dose-dependent manner without cytotoxicity. For molecular mechanisms, GA repressed osteoclastogenesis by blocking Akt, ERK, and JNK pathways, and suppressed osteoclastogenesis-related marker expression, including nuclear factor of the activated T-cell cytoplasmic 1 (NFATc1), c‐Fos, and cathepsin K (CTSK). In addition, we further assessed the effect of GA in an ovariectomized mouse model, which indicated that GA has a notable effect on preventing bone loss. In conclusion, GA exerts notable effects in inhibiting osteoclastogenesis and preventing ovariectomy-induced bone loss, suggesting that GA is a potential agent in osteoporosis treatment. Frontiers Media S.A. 2022-12-19 /pmc/articles/PMC9807166/ /pubmed/36601012 http://dx.doi.org/10.3389/fendo.2022.963237 Text en Copyright © 2022 Zhang, Ye, Dai, Wang, Chen, Hu, Hu and Fei https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Endocrinology
Zhang, Peng
Ye, Jiekai
Dai, Jiale
Wang, Ying
Chen, Genjun
Hu, Jinping
Hu, Qimiao
Fei, Jun
Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
title Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
title_full Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
title_fullStr Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
title_full_unstemmed Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
title_short Gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
title_sort gallic acid inhibits osteoclastogenesis and prevents ovariectomy-induced bone loss
topic Endocrinology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807166/
https://www.ncbi.nlm.nih.gov/pubmed/36601012
http://dx.doi.org/10.3389/fendo.2022.963237
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