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Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway
The effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sh...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi
2022
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807299/ https://www.ncbi.nlm.nih.gov/pubmed/36600931 http://dx.doi.org/10.1155/2022/4588999 |
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author | Deng, Haixia Tang, Zhanhong Tuo, Peng Wu, Ruihua Jia, Si Zhao, Xuan Huang, Deqing Gao, Yuguang Lan, Zhou |
author_facet | Deng, Haixia Tang, Zhanhong Tuo, Peng Wu, Ruihua Jia, Si Zhao, Xuan Huang, Deqing Gao, Yuguang Lan, Zhou |
author_sort | Deng, Haixia |
collection | PubMed |
description | The effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sham group, model group, conventional western medicine (CWM) group, Shenfu group, and antagonist group (n = 32 per group). After 3 hours, 24 hours, 3 days, and 7 days of drug administration, the modified Neurological Severity Score tests were performed. The ultrastructure of the brain and hippocampus was observed by electron microscopy. Real-time quantitative polymerase chain reaction (PCR), western blotting, and immunohistochemistry were used to detect Nogo receptor (NgR) expression in the hippocampus and cerebral cortex, and Nogo–NgR expression in CA/CPR model. Neurological deficits in the model group were severe at 3 hours, 24 hours, 3 days, and 7 days after the recovery of natural circulation, whereas the neurological deficits in CWM, antagonist, and Shenfu group were relatively mild. The ultrastructure of neuronal cells in Shenfu group had relatively complete cell membranes and more vesicles than those in the model group. The results of PCR and western blotting showed lower messenger ribonucleic acid and protein expression of NgR in Shenfu group than the model group and CWM group. Immunohistochemical examination indicated a reduction of Nogo–NgR expression in Shenfu group and antagonist group. Our results suggested that Shenfu injection reduced brain injury by attenuating Nogo–NgR signaling pathway and promoting axonal regeneration. |
format | Online Article Text |
id | pubmed-9807299 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2022 |
publisher | Hindawi |
record_format | MEDLINE/PubMed |
spelling | pubmed-98072992023-01-03 Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway Deng, Haixia Tang, Zhanhong Tuo, Peng Wu, Ruihua Jia, Si Zhao, Xuan Huang, Deqing Gao, Yuguang Lan, Zhou Anal Cell Pathol (Amst) Research Article The effect of Shenfu injection on brain injury after cardiac arrest (CA) and cardiopulmonary resuscitation (CPR) along with the underlying mechanism of axonal regeneration was explored. CA/CPR model in rats was established for subsequent experiments. A total of 160 rats were randomly divided into sham group, model group, conventional western medicine (CWM) group, Shenfu group, and antagonist group (n = 32 per group). After 3 hours, 24 hours, 3 days, and 7 days of drug administration, the modified Neurological Severity Score tests were performed. The ultrastructure of the brain and hippocampus was observed by electron microscopy. Real-time quantitative polymerase chain reaction (PCR), western blotting, and immunohistochemistry were used to detect Nogo receptor (NgR) expression in the hippocampus and cerebral cortex, and Nogo–NgR expression in CA/CPR model. Neurological deficits in the model group were severe at 3 hours, 24 hours, 3 days, and 7 days after the recovery of natural circulation, whereas the neurological deficits in CWM, antagonist, and Shenfu group were relatively mild. The ultrastructure of neuronal cells in Shenfu group had relatively complete cell membranes and more vesicles than those in the model group. The results of PCR and western blotting showed lower messenger ribonucleic acid and protein expression of NgR in Shenfu group than the model group and CWM group. Immunohistochemical examination indicated a reduction of Nogo–NgR expression in Shenfu group and antagonist group. Our results suggested that Shenfu injection reduced brain injury by attenuating Nogo–NgR signaling pathway and promoting axonal regeneration. Hindawi 2022-12-26 /pmc/articles/PMC9807299/ /pubmed/36600931 http://dx.doi.org/10.1155/2022/4588999 Text en Copyright © 2022 Haixia Deng et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Deng, Haixia Tang, Zhanhong Tuo, Peng Wu, Ruihua Jia, Si Zhao, Xuan Huang, Deqing Gao, Yuguang Lan, Zhou Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
title | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
title_full | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
title_fullStr | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
title_full_unstemmed | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
title_short | Shenfu Injection Protects Brain Injury in Rats with Cardiac Arrest through Nogo/NgR Pathway |
title_sort | shenfu injection protects brain injury in rats with cardiac arrest through nogo/ngr pathway |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807299/ https://www.ncbi.nlm.nih.gov/pubmed/36600931 http://dx.doi.org/10.1155/2022/4588999 |
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