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In vivo labeling of endogenous genomic loci in C. elegans using CRISPR/dCas9

Visualization of genomic loci with open chromatin state has been reported in mammalian tissue culture cells using a CRISPR/Cas9-based system that utilizes an EGFP-tagged endonuclease-deficient Cas9 protein (dCas9::EGFP) (Chen et al. 2013). Here, we adapted this approach for use in Caenorhabditis ele...

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Detalles Bibliográficos
Autores principales:	Memar, Nadin, Sethi, Aditya, Luehr, Sebastian, Lambie, Eric J, Conradt, Barbara
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Caltech Library 2022
Materias:	New Methods
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807462/ https://www.ncbi.nlm.nih.gov/pubmed/36606081 http://dx.doi.org/10.17912/micropub.biology.000701

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