Cargando…

Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study

BACKGROUND: Past studies have shown that culturing slow-growing embryos from day 5 to day 6 may increase vitri- fication yield. This study aims to evaluate if the proportion of embryos eligible for vitrification increases by growing embryos not vitrified by day 5 to day 6. MATERIALS AND METHODS: In...

Descripción completa

Detalles Bibliográficos
Autores principales: Wu, Clara Q., Campbell, Molly, Shmorgun, Doron, Torrance, Samantha, Gale, Jenna, Léveillé, Marie-Claude
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royan Institute 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807896/
https://www.ncbi.nlm.nih.gov/pubmed/36617201
http://dx.doi.org/10.22074/IJFS.2022.535422.1195
_version_ 1784862813563387904
author Wu, Clara Q.
Campbell, Molly
Shmorgun, Doron
Torrance, Samantha
Gale, Jenna
Léveillé, Marie-Claude
author_facet Wu, Clara Q.
Campbell, Molly
Shmorgun, Doron
Torrance, Samantha
Gale, Jenna
Léveillé, Marie-Claude
author_sort Wu, Clara Q.
collection PubMed
description BACKGROUND: Past studies have shown that culturing slow-growing embryos from day 5 to day 6 may increase vitri- fication yield. This study aims to evaluate if the proportion of embryos eligible for vitrification increases by growing embryos not vitrified by day 5 to day 6. MATERIALS AND METHODS: In this retrospective cohort study, a Canadian tertiary-care clinic-based cohort was identi- fied between August 2019 and December 2020. In vitro fertilization (IVF) cycles involving autologous oocytes with at least one viable day 5 embryo were selected for inclusion. We compared embryo developmental outcomes of IVF cycles performed before and after an embryo cryopreservation policy change. Prior to March 2020, good-quality day 5 blastocysts of any stage were eligible for vitrification, and after that date, good-quality expanded blastocysts on either day 5 or day 6 were eligible. The primary outcome is the comparative proportion of embryos eligible for vitrification. The secondary outcome is to identify embryo, maternal and cycle factors that are predictive of day 6 vitrification. RESULTS: A total of 3,438 viable embryos across 679 consecutive IVF cycles were included in this study. After the policy change, we found similar mean proportions of blastocysts eligible for cryopreservation (46.9% per IVF cycle in group 2 vs. 44.4% in group 1, mean difference 0.025, 95% confidence interval -0.021 to 0.071, P=0.28). The mean number of cryopreserved embryos were significantly higher in group 2 (mean 2.2 vs. 1.7 embryos, P=0.007). Factors that predicated an embryo’s progression to day 6 included: younger age of egg provider, presence of an early blasto- cyst on day 5, and cycles involving surgically-retrieved sperm. CONCLUSION: A cryopreservation policy change to include good-quality full and expanded day 6 blastocysts while avoiding to vitrify early blastocysts on day 5 yielded comparable proportions of embryos eligible for vitrification per IVF cycle.
format Online
Article
Text
id pubmed-9807896
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Royan Institute
record_format MEDLINE/PubMed
spelling pubmed-98078962023-01-06 Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study Wu, Clara Q. Campbell, Molly Shmorgun, Doron Torrance, Samantha Gale, Jenna Léveillé, Marie-Claude Int J Fertil Steril Original Article BACKGROUND: Past studies have shown that culturing slow-growing embryos from day 5 to day 6 may increase vitri- fication yield. This study aims to evaluate if the proportion of embryos eligible for vitrification increases by growing embryos not vitrified by day 5 to day 6. MATERIALS AND METHODS: In this retrospective cohort study, a Canadian tertiary-care clinic-based cohort was identi- fied between August 2019 and December 2020. In vitro fertilization (IVF) cycles involving autologous oocytes with at least one viable day 5 embryo were selected for inclusion. We compared embryo developmental outcomes of IVF cycles performed before and after an embryo cryopreservation policy change. Prior to March 2020, good-quality day 5 blastocysts of any stage were eligible for vitrification, and after that date, good-quality expanded blastocysts on either day 5 or day 6 were eligible. The primary outcome is the comparative proportion of embryos eligible for vitrification. The secondary outcome is to identify embryo, maternal and cycle factors that are predictive of day 6 vitrification. RESULTS: A total of 3,438 viable embryos across 679 consecutive IVF cycles were included in this study. After the policy change, we found similar mean proportions of blastocysts eligible for cryopreservation (46.9% per IVF cycle in group 2 vs. 44.4% in group 1, mean difference 0.025, 95% confidence interval -0.021 to 0.071, P=0.28). The mean number of cryopreserved embryos were significantly higher in group 2 (mean 2.2 vs. 1.7 embryos, P=0.007). Factors that predicated an embryo’s progression to day 6 included: younger age of egg provider, presence of an early blasto- cyst on day 5, and cycles involving surgically-retrieved sperm. CONCLUSION: A cryopreservation policy change to include good-quality full and expanded day 6 blastocysts while avoiding to vitrify early blastocysts on day 5 yielded comparable proportions of embryos eligible for vitrification per IVF cycle. Royan Institute 2023 2022-12-25 /pmc/articles/PMC9807896/ /pubmed/36617201 http://dx.doi.org/10.22074/IJFS.2022.535422.1195 Text en Any use, distribution, reproduction or abstract of this publication in any medium, with the exception of commercial purposes, is permitted provided the original work is properly cited. https://creativecommons.org/licenses/by-nc/3.0/This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial 3.0 (CC BY-NC 3.0) License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Original Article
Wu, Clara Q.
Campbell, Molly
Shmorgun, Doron
Torrance, Samantha
Gale, Jenna
Léveillé, Marie-Claude
Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study
title Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study
title_full Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study
title_fullStr Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study
title_full_unstemmed Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study
title_short Comparative Embryo Development Outcomes following Extending Embryo Culture to Day 6: A Retrospective Cohort Study
title_sort comparative embryo development outcomes following extending embryo culture to day 6: a retrospective cohort study
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807896/
https://www.ncbi.nlm.nih.gov/pubmed/36617201
http://dx.doi.org/10.22074/IJFS.2022.535422.1195
work_keys_str_mv AT wuclaraq comparativeembryodevelopmentoutcomesfollowingextendingembryoculturetoday6aretrospectivecohortstudy
AT campbellmolly comparativeembryodevelopmentoutcomesfollowingextendingembryoculturetoday6aretrospectivecohortstudy
AT shmorgundoron comparativeembryodevelopmentoutcomesfollowingextendingembryoculturetoday6aretrospectivecohortstudy
AT torrancesamantha comparativeembryodevelopmentoutcomesfollowingextendingembryoculturetoday6aretrospectivecohortstudy
AT galejenna comparativeembryodevelopmentoutcomesfollowingextendingembryoculturetoday6aretrospectivecohortstudy
AT leveillemarieclaude comparativeembryodevelopmentoutcomesfollowingextendingembryoculturetoday6aretrospectivecohortstudy