Cargando…

WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour

Salt stress is one of the major environmental stress factors that affect and limit wheat production worldwide. Therefore, properly evaluating wheat genotypes during the germination stage could be one of the effective ways to improve yield. Currently, phenotypic identification platforms are widely us...

Descripción completa

Detalles Bibliográficos
Autores principales: Fu, Xiuqing, Han, Bing, Liu, Shouyang, Zhou, Jiayi, Zhang, Hongwen, Wang, Hongbiao, Zhang, Hui, Ouyang, Zhiqian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2022
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807913/
https://www.ncbi.nlm.nih.gov/pubmed/36605955
http://dx.doi.org/10.3389/fpls.2022.1074360
_version_ 1784862817725186048
author Fu, Xiuqing
Han, Bing
Liu, Shouyang
Zhou, Jiayi
Zhang, Hongwen
Wang, Hongbiao
Zhang, Hui
Ouyang, Zhiqian
author_facet Fu, Xiuqing
Han, Bing
Liu, Shouyang
Zhou, Jiayi
Zhang, Hongwen
Wang, Hongbiao
Zhang, Hui
Ouyang, Zhiqian
author_sort Fu, Xiuqing
collection PubMed
description Salt stress is one of the major environmental stress factors that affect and limit wheat production worldwide. Therefore, properly evaluating wheat genotypes during the germination stage could be one of the effective ways to improve yield. Currently, phenotypic identification platforms are widely used in the seed breeding process, which can improve the speed of detection compared with traditional methods. We developed the Wheat Seed Vigour Assessment System (WSVAS), which enables rapid and accurate detection of wheat seed germination using the lightweight convolutional neural network YOLOv4. The WSVAS system can automatically acquire, process and analyse image data of wheat varieties to evaluate the response of wheat seeds to salt stress under controlled environments. The WSVAS image acquisition system was set up to continuously acquire images of seeds of four wheat varieties under three types of salt stress. In this paper, we verified the accuracy of WSVAS by comparing manual scoring. The cumulative germination curves of wheat seeds of four genotypes under three salt stresses were also investigated. In this study, we compared three models, VGG16 + Faster R-CNN, ResNet50 + Faster R-CNN and YOLOv4. We found that YOLOv4 was the best model for wheat seed germination target detection, and the results showed that the model achieved an average detection accuracy (mAP) of 97.59%, a recall rate (Recall) of 97.35% and the detection speed was up to 6.82 FPS. This proved that the model could effectively detect the number of germinating seeds in wheat. In addition, the germination rate and germination index of the two indicators were highly correlated with germination vigour, indicating significant differences in salt tolerance amongst wheat varieties. WSVAS can quantify plant stress caused by salt stress and provides a powerful tool for salt-tolerant wheat breeding.
format Online
Article
Text
id pubmed-9807913
institution National Center for Biotechnology Information
language English
publishDate 2022
publisher Frontiers Media S.A.
record_format MEDLINE/PubMed
spelling pubmed-98079132023-01-04 WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour Fu, Xiuqing Han, Bing Liu, Shouyang Zhou, Jiayi Zhang, Hongwen Wang, Hongbiao Zhang, Hui Ouyang, Zhiqian Front Plant Sci Plant Science Salt stress is one of the major environmental stress factors that affect and limit wheat production worldwide. Therefore, properly evaluating wheat genotypes during the germination stage could be one of the effective ways to improve yield. Currently, phenotypic identification platforms are widely used in the seed breeding process, which can improve the speed of detection compared with traditional methods. We developed the Wheat Seed Vigour Assessment System (WSVAS), which enables rapid and accurate detection of wheat seed germination using the lightweight convolutional neural network YOLOv4. The WSVAS system can automatically acquire, process and analyse image data of wheat varieties to evaluate the response of wheat seeds to salt stress under controlled environments. The WSVAS image acquisition system was set up to continuously acquire images of seeds of four wheat varieties under three types of salt stress. In this paper, we verified the accuracy of WSVAS by comparing manual scoring. The cumulative germination curves of wheat seeds of four genotypes under three salt stresses were also investigated. In this study, we compared three models, VGG16 + Faster R-CNN, ResNet50 + Faster R-CNN and YOLOv4. We found that YOLOv4 was the best model for wheat seed germination target detection, and the results showed that the model achieved an average detection accuracy (mAP) of 97.59%, a recall rate (Recall) of 97.35% and the detection speed was up to 6.82 FPS. This proved that the model could effectively detect the number of germinating seeds in wheat. In addition, the germination rate and germination index of the two indicators were highly correlated with germination vigour, indicating significant differences in salt tolerance amongst wheat varieties. WSVAS can quantify plant stress caused by salt stress and provides a powerful tool for salt-tolerant wheat breeding. Frontiers Media S.A. 2022-12-20 /pmc/articles/PMC9807913/ /pubmed/36605955 http://dx.doi.org/10.3389/fpls.2022.1074360 Text en Copyright © 2022 Fu, Han, Liu, Zhou, Zhang, Wang, Zhang and Ouyang https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Fu, Xiuqing
Han, Bing
Liu, Shouyang
Zhou, Jiayi
Zhang, Hongwen
Wang, Hongbiao
Zhang, Hui
Ouyang, Zhiqian
WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
title WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
title_full WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
title_fullStr WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
title_full_unstemmed WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
title_short WSVAS: A YOLOv4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
title_sort wsvas: a yolov4 -based phenotyping platform for automatically detecting the salt tolerance of wheat based on seed germination vigour
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9807913/
https://www.ncbi.nlm.nih.gov/pubmed/36605955
http://dx.doi.org/10.3389/fpls.2022.1074360
work_keys_str_mv AT fuxiuqing wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT hanbing wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT liushouyang wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT zhoujiayi wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT zhanghongwen wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT wanghongbiao wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT zhanghui wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour
AT ouyangzhiqian wsvasayolov4basedphenotypingplatformforautomaticallydetectingthesalttoleranceofwheatbasedonseedgerminationvigour